FastQCFastQC Report
Thu 26 May 2016
SRR1546313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546313_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2250372
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83330.37029433355907376No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67710.30088358724690856No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT35110.15601864936108342No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG207.034729E-445.010
CGCACAT207.034729E-445.035
TGCGTAT207.034729E-445.013
CGTTTTT88050.043.4667781
AATCGTG650.041.538461
CGTTCCG501.0822987E-940.530
TCGCGAG501.0822987E-940.51
ATCGCAC403.4601908E-739.37537
TCGAACA403.4601908E-739.37534
TACGCGG1150.039.1304322
CTAGACG750.039.01
AGGGCGA24000.039.06
CATACGA4400.038.86363618
AACGGGA10850.038.77884
TAGGGCG9500.038.6052635
AGCGTAA356.250957E-638.571431
TCGGCGA356.250957E-638.5714338
ACTAACG356.250957E-638.571431
CCCGAAC356.250957E-638.5714341
CGAGGGA16950.038.4955754
Produced by FastQC (version 0.11.5)