##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546313_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2250372
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19725005465763	31.0	31.0	33.0	30.0	34.0
2	31.537479581153693	31.0	31.0	34.0	30.0	34.0
3	31.606688138672183	31.0	31.0	34.0	30.0	34.0
4	35.4355084403823	37.0	35.0	37.0	33.0	37.0
5	35.35054870928007	37.0	35.0	37.0	33.0	37.0
6	35.38937784508517	37.0	35.0	37.0	33.0	37.0
7	35.8713150536889	37.0	35.0	37.0	35.0	37.0
8	35.812003970899035	37.0	35.0	37.0	35.0	37.0
9	37.559554598084226	39.0	37.0	39.0	35.0	39.0
10	37.013319575607945	39.0	37.0	39.0	33.0	39.0
11	36.667023940930655	39.0	35.0	39.0	32.0	39.0
12	36.106251766374626	37.0	35.0	39.0	32.0	39.0
13	35.85421165922789	38.0	35.0	39.0	31.0	39.0
14	36.82022172334174	39.0	35.0	41.0	31.0	41.0
15	37.077694710030165	39.0	35.0	41.0	32.0	41.0
16	37.14268174328511	39.0	35.0	41.0	32.0	41.0
17	37.08262100666023	38.0	35.0	41.0	32.0	41.0
18	37.00342698896005	38.0	35.0	40.0	32.0	41.0
19	36.93323281661876	38.0	35.0	40.0	32.0	41.0
20	36.80272905990654	38.0	35.0	40.0	32.0	41.0
21	36.677816823174126	38.0	35.0	40.0	31.0	41.0
22	36.58751042049936	38.0	35.0	40.0	31.0	41.0
23	36.51823120799583	38.0	35.0	40.0	31.0	41.0
24	36.500602122671275	38.0	35.0	40.0	31.0	41.0
25	36.4570288823359	38.0	35.0	40.0	31.0	41.0
26	36.35073179012181	38.0	35.0	40.0	31.0	41.0
27	36.24893484277266	38.0	35.0	40.0	30.0	41.0
28	36.2586141313525	38.0	35.0	40.0	30.0	41.0
29	36.23491538287892	38.0	35.0	40.0	30.0	41.0
30	36.10636952468303	38.0	35.0	40.0	30.0	41.0
31	35.92790525299817	38.0	35.0	40.0	30.0	41.0
32	35.73960527415023	38.0	34.0	40.0	29.0	41.0
33	35.53213379832312	38.0	34.0	40.0	29.0	41.0
34	35.34881210750934	38.0	34.0	40.0	27.0	41.0
35	35.1501054047953	38.0	34.0	40.0	27.0	41.0
36	34.952750922958515	37.0	34.0	40.0	25.0	41.0
37	34.78943392470223	37.0	34.0	40.0	24.0	41.0
38	34.756691338143206	37.0	34.0	40.0	24.0	41.0
39	34.72372656609663	37.0	34.0	40.0	24.0	41.0
40	34.568489565280764	37.0	33.0	40.0	23.0	41.0
41	34.50464412106087	37.0	33.0	40.0	23.0	41.0
42	34.40656211506364	37.0	33.0	40.0	23.0	41.0
43	34.37990696649265	37.0	33.0	40.0	23.0	41.0
44	34.22818405134795	37.0	33.0	40.0	23.0	41.0
45	34.17936989973213	37.0	33.0	40.0	23.0	41.0
46	34.09913072149849	36.0	33.0	40.0	23.0	41.0
47	34.0629024890107	36.0	33.0	40.0	23.0	41.0
48	33.99669343557421	36.0	33.0	40.0	23.0	41.0
49	34.02350811332526	36.0	33.0	40.0	23.0	41.0
50	33.88845177597304	36.0	33.0	40.0	23.0	41.0
51	33.69309651915328	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	2.0
13	8.0
14	27.0
15	75.0
16	195.0
17	503.0
18	1081.0
19	2210.0
20	4012.0
21	6365.0
22	9626.0
23	14408.0
24	20947.0
25	29704.0
26	38914.0
27	43854.0
28	47082.0
29	51818.0
30	60755.0
31	74458.0
32	91403.0
33	118362.0
34	194058.0
35	269922.0
36	161335.0
37	203352.0
38	310280.0
39	495554.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.813495724262474	24.023761404781077	29.504544137591477	14.658198733364971
2	30.196696368422643	26.354576043427485	31.195153512397063	12.253574075752809
3	27.536958333999888	25.09958353552213	34.09005266684797	13.273405463630015
4	24.10245950447304	28.42272299868644	32.83203843631186	14.64277906052866
5	22.646877938403073	32.12428878425433	31.823316322812406	13.405516954530183
6	20.428622467751996	40.92927747056931	28.076513571978325	10.565586489700369
7	87.99700671711166	3.762355734962931	6.757549418496142	1.4830881294292677
8	89.67850648692749	2.607524444847341	6.087749047713	1.6262200205121642
9	84.49651879778098	5.202473191099072	7.953218401224331	2.3477896098956084
10	44.30547482816174	34.47589998453589	12.505043610567498	8.71358157673487
11	35.10304074170848	22.34119514462498	28.720229366522513	13.835534747144026
12	33.429450775249606	23.15790455977945	28.957301281743643	14.455343383227307
13	20.699422139983966	37.04058706738264	28.52701686654473	13.732973926088665
14	16.67133256190532	38.986709752876415	28.89757782268887	15.444379862529395
15	15.050000622119363	25.757830260952414	44.84654092745555	14.345628189472674
16	17.02434086453262	22.65181045622679	42.44271613759859	17.881132541642003
17	17.32811286311774	22.016448836014668	30.916710659393203	29.738727641474387
18	20.723107112957326	24.598644135280743	35.261947802407775	19.416300949354152
19	25.806888816604545	25.7658733756019	29.035332824972937	19.39190498282062
20	28.483290762593917	25.083586180418173	27.734436795338723	18.698686261649186
21	21.389441390134607	28.434276644039297	30.15297026447183	20.023311701354267
22	22.333285341268024	24.51985716139376	28.33029383586358	24.816563661474635
23	17.915749040603064	30.868407534398756	28.442053136103716	22.773790288894457
24	19.04845065615818	25.418819644041076	38.11107674642237	17.421652953378373
25	16.983369860627487	28.247374211908078	34.90369592227419	19.865560005190254
26	17.633173537530684	33.64159347876707	28.851674300960017	19.87355868274223
27	17.318114516177772	32.4306381344951	30.583521302255807	19.66772604707133
28	15.432159660713873	28.200048703058872	38.29024712358668	18.077544512640575
29	15.898526999091706	24.14209739545284	38.19826233173893	21.76111327371652
30	18.23587389107223	29.397672918077543	33.019340802320684	19.347112388529542
31	24.918502363164848	27.263536873014772	28.592028340203306	19.225932423617074
32	26.035339934908542	28.059316415241568	27.965820762078447	17.939522887771446
33	24.53381041001221	28.032120911564846	28.248707324833404	19.18536135358954
34	18.792537411592395	27.481456399208664	30.939640201708873	22.78636598749007
35	19.581118144022412	26.733491173903694	32.2005872806807	21.484803401393194
36	25.558752064103178	27.136980019303476	28.65859511227477	18.645672804318576
37	19.653506175867815	30.695813847666077	31.30597963358947	18.344700342876642
38	19.831832248179413	31.339263019625204	28.490445135293186	20.3384595969022
39	19.52037263172489	31.648145284424086	29.46886114829015	19.362620935560876
40	22.283204732373136	27.843263247143142	27.91534021930596	21.95819180117776
41	17.753509197590443	26.755309788781588	29.078881180533706	26.41229983309426
42	20.758479042576074	28.16627650895052	27.075523513445777	23.99972093502763
43	21.361357144507664	28.062604760457383	28.025055413060596	22.550982681974357
44	20.102409734923825	30.411060926815654	29.062306143162107	20.42422319509841
45	17.89544128704054	34.33498994832854	26.282810130947237	21.486758633683674
46	21.06820561222767	32.194854895101784	28.173430881649793	18.563508611020758
47	20.791940177001848	27.922316843615192	29.381097880705948	21.90464509867702
48	21.517242482576215	25.074787635110994	31.71520086456817	21.692769017744624
49	19.698965326621554	25.15535209289842	32.98370225011687	22.161980330363157
50	18.443572884838595	32.05470028955213	29.000894074401923	20.50083275120736
51	18.122337106931656	32.118556398675416	27.385516705682438	22.373589788710486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	677.0
1	1146.5
2	1616.0
3	8921.0
4	16226.0
5	12659.0
6	9092.0
7	9399.5
8	9707.0
9	10105.0
10	10503.0
11	10682.0
12	10861.0
13	10709.0
14	10557.0
15	10046.5
16	9536.0
17	9438.5
18	9341.0
19	9274.0
20	9207.0
21	9958.5
22	10710.0
23	11668.5
24	12627.0
25	15225.5
26	22152.5
27	26481.0
28	31703.0
29	36925.0
30	43748.0
31	50571.0
32	59969.5
33	69368.0
34	81336.0
35	93304.0
36	100458.5
37	107613.0
38	117437.5
39	127262.0
40	147844.0
41	168426.0
42	192410.5
43	216395.0
44	219473.5
45	222552.0
46	214635.5
47	206719.0
48	197346.5
49	187974.0
50	177318.5
51	166663.0
52	148829.5
53	130996.0
54	113340.5
55	95685.0
56	81204.5
57	66724.0
58	57187.0
59	47650.0
60	41224.0
61	34798.0
62	29119.5
63	23441.0
64	18753.5
65	14066.0
66	11258.5
67	8451.0
68	6279.0
69	4107.0
70	3078.5
71	2050.0
72	1876.0
73	1702.0
74	1273.0
75	662.5
76	481.0
77	357.0
78	233.0
79	178.5
80	124.0
81	139.0
82	154.0
83	102.5
84	51.0
85	52.5
86	54.0
87	32.0
88	10.0
89	7.0
90	4.0
91	3.5
92	3.0
93	5.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2250372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.221516310441817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72305135208862	15.516194481928036
2	6.911805287769073	2.6571075614690245
3	2.1963556250877727	1.2665185641346577
4	1.1083489842140142	0.8521659231092519
5	0.683137232129822	0.6565466724826726
6	0.49817441463509987	0.5745400581812027
7	0.4097753472731532	0.5513552464859511
8	0.32898040502719866	0.5058801776836843
9	0.28112084673347176	0.48632120466233786
>10	3.836015545175671	18.22936902582066
>50	1.7689571698691855	24.793007307809233
>100	1.2524175337512762	32.93196860112222
>500	0.001162660153532126	0.14700574328618082
>1k	2.3253203070642522E-4	0.15692829573663145
>5k	4.6506406141285045E-4	0.6750911360883172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8333	0.37029433355907376	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6771	0.30088358724690856	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3511	0.15601864936108342	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04923630404217614	0.0
2	0.0	0.0	0.0	0.17663746260618243	0.0
3	0.0	0.0	0.0	0.26049026560941924	0.0
4	0.0	0.0	0.0	0.3840253966899695	0.0
5	0.0	0.0	0.0	0.6422493703263282	0.0
6	0.0	0.0	0.0	0.9376671945793851	0.0
7	0.0	0.0	0.0	1.1278579719264192	0.0
8	0.0	0.0	0.0	1.6706126809256425	0.0
9	0.0	0.0	0.0	1.950833017829941	0.0
10	0.0	0.0	0.0	2.383561473391955	0.0
11	0.0	0.0	0.0	2.7162175853592205	0.0
12	0.0	0.0	0.0	3.0277660760087666	0.0
13	0.0	0.0	0.0	3.1483683586535913	0.0
14	0.0	0.0	0.0	3.1946273771625315	0.0
15	0.0	0.0	0.0	3.2612830234290153	0.0
16	0.0	0.0	0.0	3.402637430611472	0.0
17	0.0	0.0	0.0	3.590872975668023	0.0
18	0.0	0.0	0.0	3.8585176139767112	0.0
19	0.0	0.0	0.0	3.9837413547626794	0.0
20	0.0	0.0	0.0	4.117763640855823	0.0
21	0.0	0.0	0.0	4.277914940285428	0.0
22	0.0	0.0	0.0	4.452197236723529	0.0
23	0.0	0.0	0.0	4.659674045002338	0.0
24	0.0	0.0	0.0	4.80085070379475	0.0
25	0.0	0.0	0.0	4.9157650379581685	0.0
26	0.0	0.0	0.0	5.025213609127736	0.0
27	0.0	0.0	0.0	5.143905096579588	0.0
28	0.0	0.0	0.0	5.268640029292935	0.0
29	0.0	0.0	0.0	5.408483575160018	0.0
30	0.0	0.0	0.0	5.600762896090068	0.0
31	0.0	0.0	0.0	5.750782537287169	0.0
32	0.0	0.0	0.0	5.884449326600224	0.0
33	0.0	0.0	0.0	6.0256704224901485	0.0
34	0.0	0.0	0.0	6.173201586226633	0.0
35	0.0	0.0	0.0	6.360903886113052	0.0
36	0.0	0.0	0.0	6.508123990166959	0.0
37	0.0	0.0	0.0	6.661032042702273	0.0
38	0.0	0.0	0.0	6.811318306484439	0.0
39	0.0	0.0	0.0	6.9594271524885665	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTACG	20	7.034729E-4	45.0	10
CGCACAT	20	7.034729E-4	45.0	35
TGCGTAT	20	7.034729E-4	45.0	13
CGTTTTT	8805	0.0	43.466778	1
AATCGTG	65	0.0	41.53846	1
CGTTCCG	50	1.0822987E-9	40.5	30
TCGCGAG	50	1.0822987E-9	40.5	1
ATCGCAC	40	3.4601908E-7	39.375	37
TCGAACA	40	3.4601908E-7	39.375	34
TACGCGG	115	0.0	39.130432	2
CTAGACG	75	0.0	39.0	1
AGGGCGA	2400	0.0	39.0	6
CATACGA	440	0.0	38.863636	18
AACGGGA	1085	0.0	38.7788	4
TAGGGCG	950	0.0	38.605263	5
AGCGTAA	35	6.250957E-6	38.57143	1
TCGGCGA	35	6.250957E-6	38.57143	38
ACTAACG	35	6.250957E-6	38.57143	1
CCCGAAC	35	6.250957E-6	38.57143	41
CGAGGGA	1695	0.0	38.495575	4
>>END_MODULE