FastQCFastQC Report
Thu 26 May 2016
SRR1546312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546312_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2900176
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114650.39532083570100573No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC100880.3478409586176839No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG406.8212103E-945.01
CGTTACA207.0350664E-445.036
GTCGTAA207.0350664E-445.018
TATTACG502.1827873E-1145.01
ACCGTTA253.8922823E-545.019
GTAACGA253.8922823E-545.042
TACGTCC207.0350664E-445.030
TACGATA302.166651E-644.99999613
CGTTTTT102800.042.461091
ATAGGGT11800.041.7584724
CTAAACG650.041.538461
GCGATAT3750.041.3999989
AATACGG3050.041.3114742
CGACGGT4800.041.24999628
TCACGAC5000.040.94999725
TAGGGCG16150.040.681115
CGTAAGG3900.040.3846172
ATAGCGG5300.040.330192
GCGATAA2800.040.178579
ACGGATC451.9301297E-840.030
Produced by FastQC (version 0.11.5)