##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546312_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2900176
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248002879825226	31.0	31.0	33.0	30.0	34.0
2	31.592198887239945	31.0	31.0	34.0	30.0	34.0
3	31.69369583087371	31.0	31.0	34.0	30.0	34.0
4	35.528937209327985	37.0	35.0	37.0	33.0	37.0
5	35.44753525303292	37.0	35.0	37.0	33.0	37.0
6	35.48448128665295	37.0	35.0	37.0	33.0	37.0
7	35.872984949878905	37.0	35.0	37.0	35.0	37.0
8	35.791444036499854	37.0	35.0	37.0	35.0	37.0
9	37.567609000281365	39.0	37.0	39.0	35.0	39.0
10	37.0164159002764	39.0	37.0	39.0	33.0	39.0
11	36.77017739613044	39.0	35.0	39.0	32.0	39.0
12	35.91076817406944	37.0	35.0	39.0	32.0	39.0
13	35.50466178604333	37.0	35.0	39.0	31.0	39.0
14	36.3526813545109	38.0	35.0	40.0	31.0	41.0
15	36.69416580235131	38.0	35.0	41.0	31.0	41.0
16	36.822797995707845	38.0	35.0	41.0	32.0	41.0
17	36.78265870760947	38.0	35.0	40.0	32.0	41.0
18	36.72933021995907	38.0	35.0	40.0	32.0	41.0
19	36.626120966451694	38.0	35.0	40.0	31.0	41.0
20	36.47475808364734	38.0	35.0	40.0	31.0	41.0
21	36.30569627498469	37.0	35.0	40.0	31.0	41.0
22	36.21199437551376	37.0	35.0	40.0	31.0	41.0
23	36.17404840257971	37.0	35.0	40.0	31.0	41.0
24	36.156758417420185	37.0	35.0	40.0	31.0	41.0
25	36.09027521088375	37.0	35.0	40.0	31.0	41.0
26	35.98552743005942	37.0	35.0	40.0	31.0	41.0
27	35.86796835778242	37.0	34.0	40.0	30.0	41.0
28	35.90134771131132	37.0	35.0	40.0	30.0	41.0
29	35.866649127501226	37.0	35.0	40.0	30.0	41.0
30	35.73852655838818	36.0	35.0	40.0	30.0	41.0
31	35.51948433474382	36.0	34.0	40.0	30.0	41.0
32	35.35088491181225	36.0	34.0	40.0	29.0	41.0
33	35.17276710103111	36.0	34.0	40.0	28.0	41.0
34	34.98384270471861	36.0	34.0	40.0	27.0	41.0
35	34.80133826360883	36.0	34.0	40.0	26.0	41.0
36	34.582392585829275	36.0	33.0	40.0	25.0	41.0
37	34.38079861360138	36.0	33.0	40.0	24.0	41.0
38	34.31931889650835	35.0	33.0	40.0	24.0	41.0
39	34.2860409161375	35.0	33.0	40.0	24.0	41.0
40	34.07609331295756	35.0	33.0	40.0	23.0	41.0
41	33.981970749361416	35.0	33.0	40.0	23.0	41.0
42	33.87695057127567	35.0	33.0	40.0	23.0	41.0
43	33.80473805727652	35.0	33.0	40.0	23.0	41.0
44	33.63274504719713	35.0	33.0	40.0	23.0	41.0
45	33.61033571755645	35.0	33.0	39.0	23.0	41.0
46	33.54711472683037	35.0	33.0	39.0	23.0	41.0
47	33.47688519593294	35.0	33.0	39.0	23.0	41.0
48	33.39911819144769	35.0	33.0	39.0	23.0	41.0
49	33.41162053613298	35.0	33.0	39.0	23.0	41.0
50	33.2572374917936	35.0	32.0	39.0	23.0	41.0
51	33.033813465113845	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	2.0
11	4.0
12	5.0
13	10.0
14	37.0
15	88.0
16	254.0
17	659.0
18	1488.0
19	2992.0
20	5182.0
21	8504.0
22	12762.0
23	18511.0
24	26710.0
25	38217.0
26	49891.0
27	58088.0
28	62398.0
29	70489.0
30	83639.0
31	103231.0
32	130511.0
33	172575.0
34	300740.0
35	422918.0
36	210681.0
37	259046.0
38	365817.0
39	494651.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.8447652832104	22.903265181147628	29.993110762933007	14.258858772708965
2	30.32288385256619	25.40528574817528	31.228035815757387	13.043794583501139
3	28.490167493283163	25.028929278774804	32.841489619940305	13.639413608001721
4	26.052212003685295	27.91499550372115	30.958465968961885	15.07432652363167
5	22.578767633412593	32.10563772681382	31.178625021378014	14.136969618395574
6	20.72232857592091	40.06408576582938	27.91702986301521	11.296555795234497
7	86.98520365660568	4.623167697408709	6.60884029107199	1.782788354913633
8	88.92156889788758	3.071089478707499	6.194796453732463	1.8125451696724613
9	84.75320118503153	3.96227677216831	8.499139362576615	2.785382680223545
10	50.88822195618473	17.795437242429422	18.467499903454137	12.848840897931712
11	47.632419549710086	20.079333116335008	18.396262847496153	13.891984486458753
12	40.638188854745366	21.65844417718097	22.81564980883919	14.887717159234473
13	23.790349275354323	37.43083178400207	23.9332371552623	14.8455817853813
14	18.328198012810258	40.642395495997484	26.907746288501112	14.121660202691148
15	16.531789794826246	21.78843628800459	46.15816419417305	15.521609722996121
16	18.121314016804497	16.75063858193434	44.30417326396743	20.82387413729374
17	18.82399550923806	17.590587605717722	28.35283100060134	35.23258588444288
18	23.816382178185048	22.13113962738813	33.35232068674453	20.70015750768229
19	30.238164856201834	23.43999122811857	25.11526886644121	21.206575049238392
20	31.898857172806068	22.904747849785668	22.957503268767137	22.23889170864113
21	24.314351956570913	26.883368457638433	27.77910719901137	21.023172386779283
22	23.636220698330032	23.051876851611762	25.99462929146369	27.317273158594514
23	21.275295016578305	29.773917169164903	24.349625677889893	24.6011621363669
24	21.83595064575391	24.688329260017323	35.66259427014084	17.813125824087916
25	19.508057442031106	23.845656263619862	34.19871759507009	22.447568699278943
26	18.017768576803615	31.855377052978856	27.194349584301094	22.932504785916443
27	19.06073976200065	30.4383251223374	29.351253165325137	21.149681950336806
28	16.671574414794136	26.724584990704013	37.40324725120131	19.200593343300547
29	17.451492599069848	24.20911696393598	36.56302238208992	21.776368054904253
30	20.684779130645865	27.2594835623769	31.72876404742333	20.326973259553903
31	29.21860604321945	24.90948825174748	25.270190498783524	20.60171520624955
32	29.91777050772091	25.313049966622714	25.52665769249866	19.24252183315771
33	28.517924429413938	24.903523096529316	25.94925273500643	20.629299739050317
34	20.571579104164712	25.929012584063866	29.579446212919493	23.919962098851933
35	21.62606683180607	24.25570034370328	30.78375243433502	23.334480390155633
36	30.284920639299134	23.04273947512151	26.148585465157975	20.52375442042138
37	22.30778407931105	29.148127561913483	28.299385968299855	20.244702390475613
38	22.53656329822742	30.997980812198982	23.312205879919013	23.153250009654585
39	21.518176827889064	29.474314662282563	25.35428884315986	23.653219666668505
40	24.462101610385027	23.912410832997722	25.054100164955507	26.571387391661748
41	19.86320830184099	22.293302199590645	26.64731381819586	31.1961756803725
42	23.0065002951545	26.028937554134647	24.063780956741937	26.90078119396892
43	22.93491843253651	25.923081909511698	25.798330859920227	25.343668798031565
44	21.804814604355048	28.302178902245934	27.632805733169295	22.260200760229722
45	19.567708994212765	32.530749857939654	23.948856896960734	23.952684250886843
46	22.79275464661455	29.53624193842029	26.98891377626737	20.68208963869779
47	23.405372639453606	26.27899134397361	26.95895007751254	23.356685939060252
48	24.05971223815382	23.539640352861344	29.270292561554882	23.13035484742995
49	21.80809026762514	21.91560098421613	31.717247504979007	24.559061243179727
50	20.899386795835838	29.916942971736887	26.04924666640921	23.13442356601806
51	20.530822956951578	31.66476793132555	24.278526544595913	23.52588256712696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1152.0
1	2012.5
2	2873.0
3	11739.5
4	20606.0
5	15290.0
6	9974.0
7	10545.0
8	11116.0
9	11973.5
10	12831.0
11	13025.0
12	13219.0
13	12941.0
14	12663.0
15	12270.5
16	11878.0
17	10771.0
18	9664.0
19	9192.5
20	8721.0
21	9193.5
22	9666.0
23	9712.5
24	9759.0
25	11340.5
26	13620.0
27	14318.0
28	16820.5
29	19323.0
30	21647.0
31	23971.0
32	31504.0
33	39037.0
34	44832.5
35	50628.0
36	53464.5
37	56301.0
38	77452.0
39	98603.0
40	134305.5
41	170008.0
42	210880.0
43	251752.0
44	266675.0
45	281598.0
46	272648.5
47	263699.0
48	256581.5
49	249464.0
50	246941.5
51	244419.0
52	222758.5
53	201098.0
54	181441.0
55	161784.0
56	150887.5
57	139991.0
58	129483.5
59	118976.0
60	111680.5
61	104385.0
62	93623.5
63	82862.0
64	74814.5
65	66767.0
66	55216.5
67	43666.0
68	35204.5
69	26743.0
70	21408.0
71	16073.0
72	14616.0
73	13159.0
74	10392.0
75	5392.0
76	3159.0
77	2329.5
78	1500.0
79	1397.5
80	1295.0
81	911.0
82	527.0
83	367.0
84	207.0
85	147.0
86	87.0
87	69.0
88	51.0
89	43.0
90	35.0
91	23.5
92	12.0
93	9.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2900176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.423681376275674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.10128819157492	13.319817164639355
2	7.77612632819987	2.554252423120463
3	2.554063698007857	1.2584138517238028
4	1.2093782536751	0.7944977240702616
5	0.7173156304427702	0.5890481680307186
6	0.49835116816759845	0.4910856479687648
7	0.35305372062672613	0.4058909271397399
8	0.2793709568633814	0.36706396650475465
9	0.22669986303548434	0.3350921686686118
>10	2.328249660176447	8.920749246886576
>50	1.0198745684291255	12.321031632144162
>100	1.9195308746342343	55.77303937291875
>500	0.012892686786820418	1.2993182481878829
>1k	0.003381688337526667	0.8225440199048936
>5k	0.0	0.0
>10k+	4.227110421908334E-4	0.7481554380912463
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11465	0.39532083570100573	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10088	0.3478409586176839	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04220433518517497	0.0
2	0.0	0.0	0.0	0.18426467910912994	0.0
3	0.0	0.0	0.0	0.2638115755733445	0.0
4	0.0	0.0	0.0	0.39604492968702587	0.0
5	0.0	0.0	0.0	0.6501329574480997	0.0
6	0.0	0.0	0.0	0.9309435013599174	0.0
7	0.0	0.0	0.0	1.107794837278841	0.0
8	0.0	0.0	0.0	1.55711239593735	0.0
9	0.0	0.0	0.0	1.76796166853322	0.0
10	0.0	0.0	0.0	2.1448008672577115	0.0
11	0.0	0.0	0.0	2.4467480594281175	0.0
12	0.0	0.0	0.0	2.718938436839695	0.0
13	0.0	0.0	0.0	2.8252768107866557	0.0
14	0.0	0.0	0.0	2.867239781309824	0.0
15	0.0	0.0	0.0	2.929408422109555	0.0
16	0.0	0.0	0.0	3.0871229883979456	0.0
17	0.0	0.0	0.0	3.2618020423588088	0.0
18	0.0	0.0	0.0	3.5475088408427626	0.0
19	0.0	0.0	0.0	3.6491923248795937	0.0
20	0.0	0.0	0.0	3.766564511946861	0.0
21	0.0	0.0	0.0	3.913727994438958	0.0
22	0.0	0.0	0.0	4.058995040301002	0.0
23	0.0	0.0	0.0	4.2270882870556825	0.0
24	0.0	0.0	0.0	4.349011922035077	0.0
25	0.0	0.0	0.0	4.444661289521739	0.0
26	0.0	0.0	0.0	4.551034144134701	0.0
27	0.0	0.0	0.0	4.659096551381709	0.0
28	0.0	0.0	0.0	4.7610558807465475	0.0
29	0.0	0.0	0.0	4.875842017863744	0.0
30	0.0	0.0	0.0	5.019178146429734	0.0
31	0.0	0.0	0.0	5.148687527929339	0.0
32	0.0	0.0	0.0	5.2621978804045	0.0
33	0.0	0.0	0.0	5.385293858027927	0.0
34	0.0	0.0	0.0	5.50970010095939	0.0
35	0.0	0.0	0.0	5.673000535139936	0.0
36	0.0	0.0	0.0	5.8063027898996475	0.0
37	0.0	0.0	0.0	5.9440530505734825	0.0
38	0.0	0.0	0.0	6.086009952499435	0.0
39	0.0	0.0	0.0	6.285066837322976	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	40	6.8212103E-9	45.0	1
CGTTACA	20	7.0350664E-4	45.0	36
GTCGTAA	20	7.0350664E-4	45.0	18
TATTACG	50	2.1827873E-11	45.0	1
ACCGTTA	25	3.8922823E-5	45.0	19
GTAACGA	25	3.8922823E-5	45.0	42
TACGTCC	20	7.0350664E-4	45.0	30
TACGATA	30	2.166651E-6	44.999996	13
CGTTTTT	10280	0.0	42.46109	1
ATAGGGT	1180	0.0	41.758472	4
CTAAACG	65	0.0	41.53846	1
GCGATAT	375	0.0	41.399998	9
AATACGG	305	0.0	41.311474	2
CGACGGT	480	0.0	41.249996	28
TCACGAC	500	0.0	40.949997	25
TAGGGCG	1615	0.0	40.68111	5
CGTAAGG	390	0.0	40.384617	2
ATAGCGG	530	0.0	40.33019	2
GCGATAA	280	0.0	40.17857	9
ACGGATC	45	1.9301297E-8	40.0	30
>>END_MODULE