FastQCFastQC Report
Thu 26 May 2016
SRR1546311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546311_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1126728
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126011.1183710709239496No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17770.15771330791459873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA900.045.0000045
CGTTTTT90900.043.8613851
CGTTCGA603.6379788E-1241.24999614
ACGTAGG1650.040.909092
TCGTTAG501.0822987E-940.51
CGGTCTA1000.040.531
ATACTAT21650.039.69976845
AGGGCGA11850.039.6835446
GTATGCG750.039.01
TATGGGA11500.038.9347844
CGTAAGG1250.037.8000032
GTTTTTT109050.037.716652
TATACTA22900.037.53275344
TCGACTA301.1399076E-437.49999623
TAGGGAT12850.037.4708145
TAGGGAC13900.037.3920865
ATGGGAC8800.037.3295445
ATTATAC23550.037.26114742
GTAGGGA13850.037.0397154
TTACGGG3950.037.0253143