##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546311_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1126728
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.093885125780137	31.0	31.0	33.0	30.0	34.0
2	31.430679809146483	31.0	31.0	34.0	30.0	34.0
3	31.405752763754872	31.0	31.0	34.0	30.0	34.0
4	35.327545778573004	37.0	35.0	37.0	33.0	37.0
5	35.28006315632522	37.0	35.0	37.0	33.0	37.0
6	35.32508111984436	37.0	35.0	37.0	33.0	37.0
7	35.74591560696104	37.0	35.0	37.0	35.0	37.0
8	35.64390873396241	37.0	35.0	37.0	35.0	37.0
9	37.354792816012385	39.0	37.0	39.0	35.0	39.0
10	36.87418170135117	39.0	37.0	39.0	32.0	39.0
11	36.634663379271664	39.0	35.0	39.0	32.0	39.0
12	36.30523427127044	38.0	35.0	39.0	32.0	39.0
13	36.17975145731712	38.0	35.0	39.0	32.0	39.0
14	37.21895879040904	39.0	35.0	41.0	32.0	41.0
15	37.361910771721305	39.0	35.0	41.0	32.0	41.0
16	37.354618860985084	39.0	35.0	41.0	32.0	41.0
17	37.267142557919925	39.0	35.0	41.0	32.0	41.0
18	37.09890319580236	39.0	35.0	40.0	32.0	41.0
19	36.95692660517889	38.0	35.0	40.0	31.0	41.0
20	36.7418099133065	38.0	35.0	40.0	31.0	41.0
21	36.61830539402589	38.0	35.0	40.0	31.0	41.0
22	36.508356053989964	38.0	35.0	40.0	31.0	41.0
23	36.40797601550685	38.0	35.0	40.0	31.0	41.0
24	36.36231725846877	38.0	35.0	40.0	30.0	41.0
25	36.29327131304095	38.0	35.0	40.0	30.0	41.0
26	36.171556045469714	38.0	35.0	40.0	30.0	41.0
27	36.06819569585561	38.0	35.0	40.0	30.0	41.0
28	35.976600386251164	38.0	35.0	40.0	30.0	41.0
29	35.83789344012042	38.0	35.0	40.0	30.0	41.0
30	35.63148781249778	38.0	34.0	40.0	29.0	41.0
31	35.38845488884629	38.0	34.0	40.0	27.0	41.0
32	35.12313530861042	38.0	34.0	40.0	25.0	41.0
33	34.783294637215015	38.0	33.0	40.0	24.0	41.0
34	34.42153385732848	37.0	33.0	40.0	23.0	41.0
35	34.12300750491689	37.0	33.0	40.0	21.0	41.0
36	33.88334451615652	37.0	33.0	40.0	20.0	41.0
37	33.70927943567569	37.0	33.0	40.0	18.0	41.0
38	33.61785098089335	37.0	33.0	40.0	18.0	41.0
39	33.519972877216155	37.0	32.0	40.0	18.0	41.0
40	33.3434182872885	37.0	32.0	40.0	18.0	41.0
41	33.183779048714506	36.0	32.0	40.0	17.0	41.0
42	33.0225094255224	36.0	31.0	40.0	16.0	41.0
43	32.988588195198844	36.0	31.0	40.0	16.0	41.0
44	32.78895172570487	36.0	31.0	40.0	15.0	41.0
45	32.750110053180535	35.0	31.0	40.0	15.0	41.0
46	32.63312263474415	35.0	31.0	40.0	15.0	41.0
47	32.56022837810013	35.0	31.0	40.0	15.0	41.0
48	32.45625741083917	35.0	31.0	40.0	15.0	41.0
49	32.41845769342734	35.0	31.0	39.0	15.0	41.0
50	32.30279091315739	35.0	31.0	39.0	15.0	41.0
51	32.10793909443983	35.0	30.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	0.0
13	5.0
14	20.0
15	72.0
16	168.0
17	457.0
18	959.0
19	1858.0
20	3161.0
21	4972.0
22	7209.0
23	10347.0
24	14676.0
25	21510.0
26	27838.0
27	30487.0
28	30151.0
29	31231.0
30	34440.0
31	39036.0
32	46817.0
33	58294.0
34	85198.0
35	108590.0
36	91557.0
37	113985.0
38	156292.0
39	207371.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.22152995221562	22.848105310243465	26.42119482253037	18.509169915010542
2	35.15249465709559	23.66596019624967	28.110511143772055	13.071034002882683
3	27.876026867176463	23.952364723340505	34.95732776677246	13.214280642710573
4	24.26264369040265	26.820048849411748	34.40999069873119	14.507316761454407
5	21.7873346539715	30.642089306380953	33.26756768270603	14.303008356941515
6	20.113550031595913	38.818951867708975	30.889531457459118	10.177966643235989
7	82.09985018567036	4.093800810843433	11.734509127313778	2.0718398761724215
8	82.54787313353356	3.6284711128151605	11.654188056034819	2.169467697616461
9	77.05062801314958	5.670046364339929	13.57079969611122	3.708525926399273
10	38.153396383155474	29.87624342343494	19.444355691879494	12.526004501530094
11	30.454910146903245	23.847725449265482	28.88168218061502	16.815682223216253
12	28.204855120312978	21.94886432217891	33.3213517370652	16.52492882044291
13	22.578652523057915	28.31029316747254	31.80173032000625	17.309323989463294
14	18.946453802514892	30.308379662172236	32.254989669201436	18.49017686611143
15	17.444849156140613	23.577385136430443	40.78357864542285	18.194187062006094
16	19.75534467946124	21.262096974602564	37.50239631925363	21.48016202668257
17	20.268245752302242	21.652342002683877	32.18221256594316	25.897199679070727
18	22.73281572837455	22.666073799532807	34.42623241811688	20.17487805397576
19	25.218863825164544	25.589760794086946	28.978422476409566	20.212952904338934
20	28.110244886077208	22.71861531798269	29.197641311833912	19.97349848410619
21	24.187381515325793	24.713950483168965	30.036441803168113	21.06222619833713
22	23.503187992132972	23.329765480222378	29.254886716226096	23.91215981141855
23	21.207247889464007	25.756083100801614	29.431681825604763	23.604987184129623
24	21.29324912489971	23.15403540162311	35.72228612406899	19.8304293494082
25	21.004448278555248	26.215998892367992	31.797736454583536	20.981816374493224
26	21.137665878543892	26.86593392549045	29.943872877926168	22.05252731803949
27	20.20035004011616	25.976899482395044	31.37039285435349	22.452357623135306
28	19.64413771557998	25.10570430485441	33.787568960742966	21.462589018822644
29	19.916075574584106	23.065637846933775	33.7993730518812	23.218913526600918
30	21.580186167380237	25.272736632088666	32.938118161614874	20.208959038916223
31	24.129958605803708	26.147570664792212	29.46594031567512	20.256530413728957
32	24.458875611505174	25.339389808365464	28.422742667263083	21.77899191286628
33	23.81843710283227	27.33561249920123	27.979512357907144	20.866438040059357
34	20.91587321873602	25.7929154152555	30.24066145511605	23.050549910892425
35	21.407207418294387	26.81179486087148	30.099101114022197	21.681896606811936
36	24.03712342286692	28.137136913256793	26.886702025688543	20.939037638187745
37	21.380137885984904	29.28834643321192	28.4569124047685	20.87460327603468
38	21.471464275317555	28.242397455286454	28.72512265604476	21.561015613351227
39	21.230412308915728	29.584158732187362	27.11213354065933	22.07329541823759
40	23.23710780241549	26.241115868248592	28.260147968276282	22.261628361059635
41	20.751059705625494	26.270492967246756	28.396560660603093	24.581886666524664
42	22.064331409177726	27.562907818035942	26.32738336137914	24.04537741140719
43	21.88505122798049	26.44045412912433	28.385910352809198	23.288584290085986
44	21.715622581492607	27.055243146526937	28.689976640324904	22.539157631655556
45	20.697808166656017	29.60324053365142	26.78605661703623	22.91289468265633
46	21.771181687150758	28.425760254471356	28.32884245354691	21.474215604830977
47	21.36735751663223	28.286063717241426	28.503507501366787	21.84307126475955
48	21.704883521133763	25.364329279116166	29.264471993240605	23.666315206509466
49	21.117519046300437	25.669371844846317	30.14250111828232	23.070607990570927
50	20.44335456294687	27.96229436030701	29.49540616723823	22.098944909507885
51	20.173103002676775	27.6432288893149	29.139064618967485	23.04460348904083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	535.0
1	850.5
2	1166.0
3	11219.5
4	21273.0
5	15279.0
6	9285.0
7	9268.0
8	9251.0
9	9387.0
10	9523.0
11	9571.0
12	9619.0
13	9339.0
14	9059.0
15	8738.0
16	8417.0
17	8027.0
18	7637.0
19	7374.5
20	7112.0
21	7074.5
22	7037.0
23	7112.0
24	7187.0
25	7597.0
26	9096.0
27	10185.0
28	11873.0
29	13561.0
30	15279.5
31	16998.0
32	20513.0
33	24028.0
34	28991.5
35	33955.0
36	37834.0
37	41713.0
38	44224.5
39	46736.0
40	51680.0
41	56624.0
42	62312.0
43	68000.0
44	69474.0
45	70948.0
46	74044.5
47	77141.0
48	77618.0
49	78095.0
50	77075.0
51	76055.0
52	72250.5
53	68446.0
54	63001.5
55	57557.0
56	56032.5
57	54508.0
58	51769.0
59	49030.0
60	46593.5
61	44157.0
62	40443.0
63	36729.0
64	32548.5
65	28368.0
66	24381.5
67	20395.0
68	17044.0
69	13693.0
70	11538.0
71	9383.0
72	7893.5
73	6404.0
74	5166.5
75	3124.0
76	2319.0
77	1853.0
78	1387.0
79	1030.5
80	674.0
81	496.5
82	319.0
83	234.0
84	149.0
85	103.5
86	58.0
87	43.0
88	28.0
89	22.5
90	17.0
91	14.5
92	12.0
93	10.5
94	9.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1126728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.463066743981972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5497895134891	23.770280699566968
2	6.462280164329734	4.0664630425723445
3	2.9914618524844596	2.8236169178038355
4	1.9745977534803898	2.4850760364108146
5	1.4177749174424048	2.230377342771696
6	1.1317618994954577	2.136522010927284
7	0.9212188193195369	2.0289058438644
8	0.8306211825593615	2.090711176466434
9	0.6794630972306365	1.9240193500416207
>10	7.854723412833129	50.6246114453501
>50	0.16592170479935406	3.1737463454039494
>100	0.01897001013863966	1.1616395855222097
>500	5.662689591342828E-4	0.10306878835562112
>1k	5.662689591342828E-4	0.258429174606445
>5k	0.0	0.0
>10k+	2.831344795671414E-4	1.1225322403363713
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12601	1.1183710709239496	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1777	0.15771330791459873	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.049435178676663756	0.0
2	0.0	0.0	0.0	0.1602871322981234	0.0
3	0.0	0.0	0.0	0.27859430137530977	0.0
4	0.0	0.0	0.0	0.372405762526537	0.0
5	0.0	0.0	0.0	0.6178066046108732	0.0
6	0.0	0.0	0.0	1.0278434546758402	0.0
7	0.0	0.0	0.0	1.2880659751066805	0.0
8	0.0	0.0	0.0	1.8110848403518862	0.0
9	0.0	0.0	0.0	2.116837426601629	0.0
10	0.0	0.0	0.0	2.50202355848084	0.0
11	0.0	0.0	0.0	2.771032582841644	0.0
12	0.0	0.0	0.0	3.0194510121342506	0.0
13	0.0	0.0	0.0	3.148142231310485	0.0
14	0.0	0.0	0.0	3.222250623043006	0.0
15	0.0	0.0	0.0	3.293696437827053	0.0
16	0.0	0.0	0.0	3.415642462067154	0.0
17	0.0	0.0	0.0	3.5593328647197904	0.0
18	0.0	0.0	0.0	3.7780191847544393	0.0
19	0.0	0.0	0.0	3.8798183767510883	0.0
20	0.0	0.0	0.0	3.9927116393663775	0.0
21	0.0	0.0	0.0	4.106847437890955	0.0
22	0.0	0.0	0.0	4.240775058399188	0.0
23	0.0	0.0	0.0	4.397867098359143	0.0
24	0.0	0.0	0.0	4.518304328995108	0.0
25	0.0	0.0	0.0	4.630487571090804	0.0
26	8.875256494912702E-5	0.0	0.0	4.724121527112134	0.0
27	8.875256494912702E-5	0.0	0.0	4.848286365475962	0.0
28	8.875256494912702E-5	0.0	0.0	4.955854474194304	0.0
29	8.875256494912702E-5	0.0	0.0	5.084811951065386	0.0
30	8.875256494912702E-5	0.0	0.0	5.267287224600791	0.0
31	8.875256494912702E-5	0.0	0.0	5.39961729893994	0.0
32	8.875256494912702E-5	0.0	0.0	5.539935104124509	0.0
33	8.875256494912702E-5	0.0	0.0	5.671200147684268	0.0
34	8.875256494912702E-5	0.0	0.0	5.805926541277043	0.0
35	8.875256494912702E-5	0.0	0.0	5.969586271043233	0.0
36	8.875256494912702E-5	0.0	0.0	6.100851314602992	0.0
37	8.875256494912702E-5	0.0	0.0	6.238684047968986	0.0
38	8.875256494912702E-5	0.0	0.0	6.3853032852649445	0.0
39	8.875256494912702E-5	0.0	0.0	6.547809231686796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	90	0.0	45.000004	5
CGTTTTT	9090	0.0	43.861385	1
CGTTCGA	60	3.6379788E-12	41.249996	14
ACGTAGG	165	0.0	40.90909	2
TCGTTAG	50	1.0822987E-9	40.5	1
CGGTCTA	100	0.0	40.5	31
ATACTAT	2165	0.0	39.699768	45
AGGGCGA	1185	0.0	39.683544	6
GTATGCG	75	0.0	39.0	1
TATGGGA	1150	0.0	38.934784	4
CGTAAGG	125	0.0	37.800003	2
GTTTTTT	10905	0.0	37.71665	2
TATACTA	2290	0.0	37.532753	44
TCGACTA	30	1.1399076E-4	37.499996	23
TAGGGAT	1285	0.0	37.470814	5
TAGGGAC	1390	0.0	37.392086	5
ATGGGAC	880	0.0	37.329544	5
ATTATAC	2355	0.0	37.261147	42
GTAGGGA	1385	0.0	37.039715	4
TTACGGG	395	0.0	37.025314	3
>>END_MODULE