FastQCFastQC Report
Thu 26 May 2016
SRR1546310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546310_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1637136
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98490.6015993784267158No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32700.19973905649866597No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT26890.16425025165899473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG406.8175723E-945.01
TGTATCG207.0341665E-445.01
CGCAATA207.0341665E-445.014
CGTTTTT73350.042.361961
TAACGCC850.042.3529412
ATAACGC850.042.3529411
CGACGGT1550.042.09677528
CGTAAGG1900.040.2631572
TCAGACG7550.039.6357622
CACAACG2900.039.5689712
CTAACGG800.039.3752
ATGGGCG5850.038.8461575
TCTAGCG356.249913E-638.571431
TGATTCG2700.038.33333615
GGCGATA7750.038.3225828
ACCCGCT7300.038.21917734
CGTTCGG5300.038.20754645
CCGCTGA7550.038.14569536
TATAGCG1600.037.968751
GCGTAAG950.037.8947371