##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546310_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1637136
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21859698888791	31.0	31.0	33.0	30.0	34.0
2	31.562326526324018	31.0	31.0	34.0	30.0	34.0
3	31.630975068656483	31.0	31.0	34.0	30.0	34.0
4	35.49441646876008	37.0	35.0	37.0	33.0	37.0
5	35.42173710675228	37.0	35.0	37.0	33.0	37.0
6	35.45811099383313	37.0	35.0	37.0	33.0	37.0
7	35.83919967553093	37.0	35.0	37.0	35.0	37.0
8	35.74481350358187	37.0	35.0	37.0	35.0	37.0
9	37.483613456670675	39.0	37.0	39.0	35.0	39.0
10	37.021667717281886	39.0	37.0	39.0	33.0	39.0
11	36.74007901603776	39.0	35.0	39.0	32.0	39.0
12	36.25588344523607	38.0	35.0	39.0	32.0	39.0
13	36.062159771698866	38.0	35.0	39.0	32.0	39.0
14	37.06085322172379	39.0	35.0	41.0	32.0	41.0
15	37.255897494160536	39.0	35.0	41.0	32.0	41.0
16	37.30972381036151	39.0	35.0	41.0	32.0	41.0
17	37.232352107583	39.0	35.0	41.0	32.0	41.0
18	37.147294421477504	39.0	35.0	41.0	32.0	41.0
19	37.0359188240928	38.0	35.0	41.0	32.0	41.0
20	36.89690654899776	38.0	35.0	41.0	32.0	41.0
21	36.765866733124184	38.0	35.0	40.0	31.0	41.0
22	36.65279793493027	38.0	35.0	40.0	31.0	41.0
23	36.56738780406759	38.0	35.0	40.0	31.0	41.0
24	36.5428510520812	38.0	35.0	40.0	31.0	41.0
25	36.486327953206086	38.0	35.0	40.0	31.0	41.0
26	36.39233392949639	38.0	35.0	40.0	31.0	41.0
27	36.27846800754488	38.0	35.0	40.0	30.0	41.0
28	36.2321535901721	38.0	35.0	40.0	30.0	41.0
29	36.15191163104348	38.0	35.0	40.0	30.0	41.0
30	36.015995005912764	38.0	35.0	40.0	30.0	41.0
31	35.85151019829752	38.0	35.0	40.0	30.0	41.0
32	35.66923212243821	38.0	34.0	40.0	29.0	41.0
33	35.46496870143959	38.0	34.0	40.0	27.0	41.0
34	35.23525779165567	38.0	34.0	40.0	27.0	41.0
35	35.0276940950538	38.0	34.0	40.0	25.0	41.0
36	34.83117957213084	37.0	34.0	40.0	24.0	41.0
37	34.66948500307855	37.0	33.0	40.0	24.0	41.0
38	34.56922393741265	37.0	33.0	40.0	24.0	41.0
39	34.52152844968286	37.0	33.0	40.0	24.0	41.0
40	34.30056268996589	37.0	33.0	40.0	23.0	41.0
41	34.20758874033678	37.0	33.0	40.0	23.0	41.0
42	34.09727292051485	36.0	33.0	40.0	23.0	41.0
43	34.081877131771584	36.0	33.0	40.0	23.0	41.0
44	33.88193711457081	36.0	33.0	40.0	23.0	41.0
45	33.839296796356564	36.0	33.0	40.0	23.0	41.0
46	33.73661809403739	36.0	33.0	40.0	23.0	41.0
47	33.67871575727368	35.0	33.0	40.0	22.0	41.0
48	33.5817042689184	35.0	33.0	40.0	22.0	41.0
49	33.55835739975176	35.0	33.0	40.0	22.0	41.0
50	33.43114866449702	35.0	32.0	39.0	22.0	41.0
51	33.24110153340956	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	10.0
13	10.0
14	23.0
15	64.0
16	161.0
17	388.0
18	891.0
19	1735.0
20	3079.0
21	4829.0
22	7330.0
23	10651.0
24	15283.0
25	21488.0
26	28755.0
27	33242.0
28	35461.0
29	39122.0
30	45315.0
31	54154.0
32	67774.0
33	86479.0
34	140305.0
35	184917.0
36	129517.0
37	164972.0
38	235329.0
39	325789.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53527135192189	23.01830758104397	28.423845056244563	15.022576010789573
2	32.03741167502272	26.144071109547408	28.938463267559932	12.880053947869937
3	28.876281506240165	24.972818385277705	32.794404374468584	13.356495734013546
4	25.461965285718474	27.739723517166563	31.563474262370384	15.234836934744578
5	22.65321879184136	32.399507432491866	30.732266592390612	14.21500718327616
6	21.231162224763246	40.10051700042025	27.98203692301678	10.686283851799729
7	85.79537680437056	4.359198014092904	7.912720751360913	1.9327044301756235
8	86.75180314891371	3.723881216954486	7.474821884070719	2.049493750061082
9	81.45395373383762	5.921927072643935	9.4224303906334	3.201688802885038
10	43.47751194769402	28.85569677778755	15.134417665972771	12.532373608545655
11	36.22576255118695	23.211694080394054	24.81284389323795	15.749699475181048
12	33.65853539351648	22.09779761730241	27.95479422601421	16.288872763166896
13	23.72942748800344	31.424756403866265	27.52508038428084	17.320735723849452
14	18.709319201336967	34.75306877376101	28.612222808612113	17.92538921628991
15	17.621627036483225	23.879995308880876	40.35681824845339	18.141559406182502
16	19.515116642722415	20.651063808993268	39.36869020044761	20.46512934783671
17	19.37572687913527	21.17154591921502	30.189428367588278	29.263298834061434
18	22.78417920075058	23.602315262751535	32.11113798731443	21.50236754918345
19	27.30347387144379	24.92108169388493	26.668584650267295	21.106859784403984
20	29.643780357893295	23.46249792320247	26.108460140147184	20.78526157875705
21	24.86671846444034	26.12067659620215	27.83366806422924	21.178936875128272
22	24.24239647775139	24.18956030531367	26.689780201522662	24.87826301541228
23	22.639780690180903	28.316645654362254	26.14236080569971	22.901212849757137
24	22.266567957701746	24.267806706345716	33.72621456006099	19.739410775891557
25	20.695348462192513	26.062770594501615	31.16839407355284	22.07348686975303
26	20.382485022624877	29.987123855318064	27.40419855161697	22.226192570440084
27	20.50581014649974	28.834562308812462	28.991177275437103	21.66845026925069
28	19.06341318009011	27.209529324381116	33.73959157944117	19.987465916087608
29	19.707342578747276	24.88699778149158	33.31256535803989	22.09309428172125
30	21.66362476910898	26.352850343526747	31.201012011219593	20.782512876144683
31	26.389988369933835	26.54813039356535	26.041880454647632	21.020000781853188
32	26.970758690786838	25.737263122917092	27.15822020895026	20.133757977345805
33	25.725718571945155	26.77621162811153	26.013843688001486	21.48422611194183
34	21.25186911777641	26.460538403651256	29.266352948075173	23.021239530497162
35	22.078984274977767	25.72913917964054	29.021535168733692	23.170341376648
36	26.002971042112566	27.164756012939673	25.98843345940716	20.843839485540602
37	22.88704176073338	29.32444219661653	27.499914484807615	20.288601557842476
38	22.36552125174695	29.935142834804196	25.961007515563768	21.738328397885088
39	22.932975635500043	28.898332209419376	26.4191856999052	21.74950645517538
40	23.68660880953079	26.312047380303166	27.351545626020073	22.649798184145972
41	20.355608819303956	25.625665796854996	27.1207767711418	26.897948612699253
42	22.44920397572346	26.84621192130648	25.177077530516705	25.52750657245336
43	22.894615963487457	26.899964327948318	26.250720770907243	23.954698937656982
44	22.16217834071207	28.514552242452673	27.22547179953284	22.09779761730241
45	20.66694520186472	30.3938707596681	25.361912510628315	23.57727152783886
46	22.02633134938087	29.740840101249987	26.624971902151074	21.607856647218068
47	22.729144066222965	27.554399878812756	27.747847460443115	21.968608594521164
48	23.08238289305226	25.22154543055678	28.824300485726294	22.87177119066467
49	22.080450249704363	25.087591989914092	29.57946071676391	23.252497043617634
50	20.680627632646278	28.92740737483019	27.815160133305966	22.57680485921756
51	20.955131400201328	29.138629900020526	26.196296459182378	23.709942240595772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	586.0
1	1298.0
2	2010.0
3	10240.0
4	18470.0
5	13576.5
6	8683.0
7	8582.0
8	8481.0
9	8687.0
10	8893.0
11	8642.5
12	8392.0
13	8137.5
14	7883.0
15	7619.5
16	7356.0
17	7007.0
18	6658.0
19	6385.0
20	6112.0
21	6070.5
22	6029.0
23	6596.5
24	7164.0
25	8407.0
26	11292.0
27	12934.0
28	15153.0
29	17372.0
30	21756.5
31	26141.0
32	30448.0
33	34755.0
34	40440.5
35	46126.0
36	49454.0
37	52782.0
38	58679.0
39	64576.0
40	79467.5
41	94359.0
42	105814.5
43	117270.0
44	119048.5
45	120827.0
46	123640.0
47	126453.0
48	123707.5
49	120962.0
50	117819.5
51	114677.0
52	108699.5
53	102722.0
54	97465.0
55	92208.0
56	87099.0
57	81990.0
58	79153.0
59	76316.0
60	69848.0
61	63380.0
62	56580.0
63	49780.0
64	43650.0
65	37520.0
66	32012.0
67	26504.0
68	21951.0
69	17398.0
70	15009.5
71	12621.0
72	10900.5
73	9180.0
74	7335.0
75	4252.5
76	3015.0
77	2338.5
78	1662.0
79	1250.0
80	838.0
81	636.0
82	434.0
83	347.0
84	260.0
85	177.5
86	95.0
87	76.5
88	58.0
89	49.5
90	41.0
91	29.5
92	18.0
93	10.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1637136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.01248010461408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37392692811875	17.252045072256912
2	5.871140865775512	2.5847674299854027
3	2.1324788447547904	1.408234444310257
4	1.3280889061088696	1.1693812249152067
5	0.9204907903825652	1.013114260488835
6	0.7746046773016948	1.023058202882674
7	0.5977514196250304	0.9210593862400562
8	0.5152524628013211	0.9073587669013989
9	0.47500630774613806	0.941046020894525
>10	6.9593084073233795	37.76618864927171
>50	1.8404033573535559	27.46729512193969
>100	0.2079236975025015	6.1957089519048205
>500	0.002508462834848214	0.31676432423364237
>1k	8.36154278282738E-4	0.42971476407755776
>5k	2.787180927609127E-4	0.6042633796972968
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9849	0.6015993784267158	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3270	0.19973905649866597	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2689	0.16425025165899473	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029991399614937304	0.0
2	0.0	0.0	0.0	0.11556767427996208	0.0
3	0.0	0.0	0.0	0.17084713792867545	0.0
4	0.0	0.0	0.0	0.255812589790952	0.0
5	0.0	0.0	0.0	0.4276981264842994	0.0
6	0.0	0.0	0.0	0.6663465955180266	0.0
7	0.0	0.0	0.0	0.8126997390564986	0.0
8	0.0	0.0	0.0	1.2026490163309584	0.0
9	0.0	0.0	0.0	1.4197965227079485	0.0
10	0.0	0.0	0.0	1.768454178516629	0.0
11	0.0	0.0	0.0	2.0319631356222088	0.0
12	0.0	0.0	0.0	2.2625487436596594	0.0
13	0.0	0.0	0.0	2.357776018608106	0.0
14	0.0	0.0	0.0	2.4022439186481757	0.0
15	0.0	0.0	0.0	2.4628375406807987	0.0
16	0.0	0.0	0.0	2.5843301961474183	0.0
17	0.0	0.0	0.0	2.727018402869401	0.0
18	0.0	0.0	0.0	2.923459016233227	6.10822802748214E-5
19	0.0	0.0	0.0	3.009890442822099	6.10822802748214E-5
20	0.0	0.0	0.0	3.1120810977218754	6.10822802748214E-5
21	0.0	0.0	0.0	3.2348564810742664	6.10822802748214E-5
22	0.0	0.0	0.0	3.3675882761114533	6.10822802748214E-5
23	0.0	0.0	0.0	3.5220042806462017	6.10822802748214E-5
24	0.0	0.0	0.0	3.6365946384417667	6.10822802748214E-5
25	0.0	0.0	0.0	3.731882995670488	6.10822802748214E-5
26	0.0	0.0	0.0	3.825949707293713	6.10822802748214E-5
27	0.0	0.0	0.0	3.927834950792115	6.10822802748214E-5
28	0.0	0.0	0.0	4.031064004456563	6.10822802748214E-5
29	0.0	0.0	0.0	4.140340203868218	6.10822802748214E-5
30	0.0	0.0	0.0	4.2909080247456535	6.10822802748214E-5
31	0.0	0.0	0.0	4.413683408098044	6.10822802748214E-5
32	0.0	0.0	0.0	4.534137664799992	6.10822802748214E-5
33	0.0	0.0	0.0	4.6512934783671	6.10822802748214E-5
34	0.0	0.0	0.0	4.771136912266299	6.10822802748214E-5
35	0.0	0.0	0.0	4.926163739603796	6.10822802748214E-5
36	0.0	0.0	0.0	5.060789085329502	6.10822802748214E-5
37	0.0	0.0	0.0	5.19315438668504	6.10822802748214E-5
38	0.0	0.0	0.0	5.322160162625463	6.10822802748214E-5
39	0.0	0.0	0.0	5.45489195766265	6.10822802748214E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	40	6.8175723E-9	45.0	1
TGTATCG	20	7.0341665E-4	45.0	1
CGCAATA	20	7.0341665E-4	45.0	14
CGTTTTT	7335	0.0	42.36196	1
TAACGCC	85	0.0	42.35294	12
ATAACGC	85	0.0	42.35294	11
CGACGGT	155	0.0	42.096775	28
CGTAAGG	190	0.0	40.263157	2
TCAGACG	755	0.0	39.63576	22
CACAACG	290	0.0	39.56897	12
CTAACGG	80	0.0	39.375	2
ATGGGCG	585	0.0	38.846157	5
TCTAGCG	35	6.249913E-6	38.57143	1
TGATTCG	270	0.0	38.333336	15
GGCGATA	775	0.0	38.322582	8
ACCCGCT	730	0.0	38.219177	34
CGTTCGG	530	0.0	38.207546	45
CCGCTGA	755	0.0	38.145695	36
TATAGCG	160	0.0	37.96875	1
GCGTAAG	95	0.0	37.894737	1
>>END_MODULE