FastQCFastQC Report
Thu 26 May 2016
SRR1546309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546309_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2208838
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102570.46436180471360955No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30010.135863291015457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.0346997E-445.0000041
ACGTCGA207.0346997E-445.00000414
ATACGTC207.0346997E-445.00000414
CGTTTTT84550.041.8598481
CGTAAGG2950.041.186442
GCGTAAG1600.039.3750041
ATGTCGC356.2509043E-638.5714311
CTAAGCG659.094947E-1238.0769271
CGGTCTA3050.037.6229531
TTCGAAT552.752131E-936.81818416
ATAGGGC11550.036.623384
AGGGCGA26200.036.4980936
GTACGAG2850.036.315791
ACGGGTA3550.036.1267625
TAACGCG250.002107527836.0000041
CACTACG250.002107527836.00000417
CGTCGAA250.002107527836.00000415
GCCGATT2250.036.09
GGGCGAT48900.035.935587
GTAGGGT10850.035.66824
Produced by FastQC (version 0.11.5)