##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546309_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2208838
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214372443791714	31.0	31.0	33.0	30.0	34.0
2	31.556278912260655	31.0	31.0	34.0	30.0	34.0
3	31.651707368308585	31.0	31.0	34.0	30.0	34.0
4	35.514344646370624	37.0	35.0	37.0	33.0	37.0
5	35.442167782336234	37.0	35.0	37.0	33.0	37.0
6	35.47761311603658	37.0	35.0	37.0	33.0	37.0
7	35.81575968903107	37.0	35.0	37.0	35.0	37.0
8	35.6991685220917	37.0	35.0	37.0	35.0	37.0
9	37.402929956837035	39.0	37.0	39.0	35.0	39.0
10	36.98169354203432	39.0	37.0	39.0	33.0	39.0
11	36.76744605081948	39.0	35.0	39.0	32.0	39.0
12	36.341829052198484	38.0	35.0	39.0	32.0	39.0
13	36.17085408708108	38.0	35.0	39.0	32.0	39.0
14	37.16847229176608	39.0	35.0	41.0	32.0	41.0
15	37.33816106024978	39.0	35.0	41.0	32.0	41.0
16	37.37038297964812	39.0	35.0	41.0	32.0	41.0
17	37.28750908848906	39.0	35.0	41.0	32.0	41.0
18	37.211452356397345	39.0	35.0	41.0	32.0	41.0
19	37.11009091658148	39.0	35.0	41.0	32.0	41.0
20	36.98873389537847	39.0	35.0	41.0	32.0	41.0
21	36.84601632170399	38.0	35.0	41.0	31.0	41.0
22	36.74943250704669	38.0	35.0	40.0	31.0	41.0
23	36.64282305900206	38.0	35.0	40.0	31.0	41.0
24	36.634478852681816	38.0	35.0	40.0	31.0	41.0
25	36.56333511104028	38.0	35.0	40.0	31.0	41.0
26	36.47291879259593	38.0	35.0	40.0	31.0	41.0
27	36.3682678403758	38.0	35.0	40.0	30.0	41.0
28	36.29093260800475	38.0	35.0	40.0	30.0	41.0
29	36.2056216888699	38.0	35.0	40.0	30.0	41.0
30	36.06673961603341	38.0	35.0	40.0	30.0	41.0
31	35.898563407547314	38.0	35.0	40.0	30.0	41.0
32	35.73508333340879	38.0	34.0	40.0	29.0	41.0
33	35.55708612401634	38.0	34.0	40.0	28.0	41.0
34	35.33853048525967	38.0	34.0	40.0	27.0	41.0
35	35.150162211986576	38.0	34.0	40.0	26.0	41.0
36	34.96926709880942	38.0	34.0	40.0	25.0	41.0
37	34.81552517658606	38.0	34.0	40.0	24.0	41.0
38	34.69512657786583	37.0	33.0	40.0	24.0	41.0
39	34.62627589710065	37.0	33.0	40.0	24.0	41.0
40	34.44676839134423	37.0	33.0	40.0	23.0	41.0
41	34.332804397606346	37.0	33.0	40.0	23.0	41.0
42	34.20923671179145	37.0	33.0	40.0	23.0	41.0
43	34.183515495477714	37.0	33.0	40.0	23.0	41.0
44	34.00009371443266	36.0	33.0	40.0	23.0	41.0
45	33.95572740056084	36.0	33.0	40.0	23.0	41.0
46	33.85678352147147	36.0	33.0	40.0	23.0	41.0
47	33.774943205431995	36.0	33.0	40.0	23.0	41.0
48	33.6913100915504	35.0	33.0	40.0	23.0	41.0
49	33.67617181522593	36.0	33.0	40.0	23.0	41.0
50	33.548259763730975	35.0	32.0	40.0	22.0	41.0
51	33.34274446564211	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	9.0
12	17.0
13	20.0
14	33.0
15	100.0
16	255.0
17	620.0
18	1309.0
19	2423.0
20	4283.0
21	6704.0
22	9933.0
23	14053.0
24	19970.0
25	28111.0
26	36007.0
27	41716.0
28	45213.0
29	51329.0
30	60094.0
31	73008.0
32	90800.0
33	116061.0
34	183518.0
35	238165.0
36	182795.0
37	234838.0
38	329642.0
39	437741.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.511748711313366	22.869626473285955	27.41174318804729	14.206881627353388
2	33.382891819137484	24.314277461724217	29.07927154458589	13.223559174552413
3	29.6890944469445	24.79448470191114	31.492259731134652	14.024161120009706
4	27.47322347768374	26.86285730325175	30.028730038146755	15.635189180917749
5	23.25113928681053	31.49040355155063	29.61548108100277	15.642976080636062
6	21.450916726351142	39.97495515741761	27.820012151185374	10.754115965045875
7	85.04702472521751	4.767302989173492	7.791834439646547	2.393837845962447
8	85.35003472414002	4.353058033228331	7.738457958437875	2.5584492841937707
9	79.67442610096349	6.044173452285772	10.078104415081594	4.20329603166914
10	43.017142950275215	24.89793275921548	17.487973314475756	14.59695097603355
11	35.406444474425015	24.801818874901645	23.511864609355687	16.279872041317653
12	31.199888810315652	23.060360243711852	28.685942563465495	17.053808382507
13	24.181673803148986	29.955705216951173	28.391443827025793	17.471177152874045
14	19.91762184460789	32.98897429327094	29.642327775961842	17.45107608615933
15	18.424483823621287	24.18027035029278	39.61870449530477	17.77654133078116
16	21.130657839099108	21.67257173228639	37.121780773420234	20.07498965519427
17	20.948118422446555	21.77923414935817	29.769860895185612	27.502786533009665
18	23.958932253066997	22.911730058972182	31.82116569888783	21.30817198907299
19	28.482215535951482	23.779561923509103	26.71083166805352	21.027390872485896
20	29.518235379869417	23.35472316213321	25.71193541581592	21.415106042181456
21	25.942056411561193	25.839694898403593	27.92930038327845	20.288948306756765
22	24.738210769644493	24.370958848045895	26.63024631050353	24.260584071806083
23	22.95419582604066	27.25958173483071	26.390980234856514	23.395242204272108
24	22.399741402493074	24.557165351193706	32.48803216895037	20.55506107736285
25	22.08591123477593	25.19541949205872	30.798365475421917	21.92030379774343
26	20.49923987182401	28.970662402584523	28.070098395627024	22.459999329964443
27	21.499811212954505	26.976129530549546	29.059034659852827	22.465024596643122
28	20.556464530219056	26.513171178692147	32.557706812360166	20.372657478728634
29	21.095526245021137	24.81485740466254	32.37770266538334	21.71191368493298
30	22.51663544361334	25.783420966136944	30.364019452762044	21.335924137487673
31	26.31383560043788	25.37551418438111	26.45861760799117	21.852032607189845
32	26.91935759888231	26.071988982442353	26.81237827310106	20.19627514557428
33	26.191418293238343	25.76245971863939	26.161040329802365	21.885081658319894
34	22.008902418375634	26.750671620100704	28.960747687245508	22.27967827427815
35	22.58735135849709	25.621073161544665	29.109649508021867	22.681925971936376
36	26.39002950872812	26.45110234430954	26.095213863578948	21.06365428338339
37	23.124104166987347	28.370346761509897	27.465164941928744	21.04038412957401
38	23.200931892696524	29.216221379748085	25.603054637777873	21.979792089777522
39	22.47416967654486	27.90512477601345	26.5819856413191	23.038719906122584
40	23.768651209368908	25.667704014508985	26.752663617703064	23.810981158419043
41	20.782103531359024	25.270979582930032	27.458555131702735	26.488361754008217
42	23.153350313603806	26.904372344191835	25.484394962419156	24.457882379785207
43	23.201067710714867	26.729982008639837	25.879806486487468	24.189143794157832
44	22.889953903364574	27.546655752934345	27.539140489252723	22.024249854448357
45	20.98913546398604	29.32342706889324	26.60516524978292	23.082272217337803
46	23.115773995195667	28.07095857640986	27.27660425979633	21.53666316859815
47	22.957319640462543	26.838500605295636	27.723083358761485	22.48109639548034
48	22.861341574167053	25.62184279698194	28.542201827386165	22.974613801464844
49	22.146938797684573	25.112298864833004	29.242615347979346	23.498146989503077
50	21.522175913308264	28.210624771938914	27.579070986645466	22.688128328107357
51	21.971869372040864	29.21694574251258	25.875596127918843	22.935588757527714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	725.0
1	1620.5
2	2516.0
3	11213.0
4	19910.0
5	14662.5
6	9415.0
7	9414.5
8	9414.0
9	9601.5
10	9789.0
11	9604.0
12	9419.0
13	9288.0
14	9157.0
15	8679.5
16	8202.0
17	8303.0
18	8404.0
19	8624.5
20	8845.0
21	8927.0
22	9009.0
23	10181.0
24	11353.0
25	13174.0
26	17332.0
27	19669.0
28	23158.0
29	26647.0
30	30612.5
31	34578.0
32	41841.5
33	49105.0
34	55730.5
35	62356.0
36	65978.5
37	69601.0
38	75791.5
39	81982.0
40	101931.0
41	121880.0
42	131545.0
43	141210.0
44	147967.5
45	154725.0
46	154444.0
47	154163.0
48	157229.5
49	160296.0
50	161042.0
51	161788.0
52	156813.5
53	151839.0
54	141959.0
55	132079.0
56	126265.5
57	120452.0
58	109821.5
59	99191.0
60	93001.5
61	86812.0
62	79137.0
63	71462.0
64	64388.0
65	57314.0
66	49140.5
67	40967.0
68	34605.5
69	28244.0
70	23479.0
71	18714.0
72	16078.0
73	13442.0
74	11064.0
75	6741.0
76	4796.0
77	3896.0
78	2996.0
79	2235.5
80	1475.0
81	1070.5
82	666.0
83	452.0
84	238.0
85	207.0
86	176.0
87	121.5
88	67.0
89	47.0
90	27.0
91	24.0
92	21.0
93	18.5
94	16.0
95	10.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2208838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.926086650639625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4873410040794	15.83871644476226
2	5.884896857141622	2.3452592941096153
3	1.9719761692672353	1.1788130406544597
4	1.1275287506271432	0.8986894234433557
5	0.7745614694210606	0.7716989477965402
6	0.6331182183390949	0.7569341087234028
7	0.49427433172517304	0.6894267214199954
8	0.43248713430729296	0.689422089079515
9	0.36164846808016493	0.6485614840832801
>10	5.889062359045302	30.418485923176462
>50	2.614905793229159	36.32960008467155
>100	0.32431136864722254	8.271961310753998
>500	0.002515813940701247	0.3299031219159582
>1k	0.0011435517912278396	0.36508549656979433
>5k	0.0	0.0
>10k+	2.287103582455679E-4	0.46744250883989275
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10257	0.46436180471360955	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3001	0.135863291015457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020191612060277846	0.0
2	0.0	0.0	0.0	0.07189300437605656	0.0
3	0.0	0.0	0.0	0.11042004891259567	0.0
4	0.0	0.0	0.0	0.16805216136267123	0.0
5	0.0	0.0	0.0	0.29255201151012433	0.0
6	0.0	0.0	0.0	0.4732805212514453	0.0
7	0.0	0.0	0.0	0.5920307419557251	0.0
8	0.0	0.0	0.0	0.9468779512123568	0.0
9	0.0	0.0	0.0	1.1374759036199125	0.0
10	0.0	0.0	0.0	1.4307975505673118	0.0
11	0.0	0.0	0.0	1.6205805948648113	0.0
12	0.0	0.0	0.0	1.7945182036889984	0.0
13	0.0	0.0	0.0	1.8650530278816282	0.0
14	0.0	0.0	0.0	1.8948877192442362	0.0
15	0.0	0.0	0.0	1.9346371259458592	0.0
16	0.0	0.0	0.0	2.0185273886088524	0.0
17	0.0	0.0	0.0	2.1164521798339218	0.0
18	0.0	0.0	0.0	2.256480556745221	0.0
19	0.0	0.0	0.0	2.321446842185801	0.0
20	0.0	0.0	0.0	2.401443654989637	0.0
21	0.0	0.0	0.0	2.4947053609182746	0.0
22	0.0	0.0	0.0	2.5874690674463223	0.0
23	0.0	0.0	0.0	2.6938598484814187	0.0
24	0.0	0.0	0.0	2.7739019339580357	0.0
25	4.5272672780892035E-5	0.0	0.0	2.8433502140039244	0.0
26	4.5272672780892035E-5	0.0	0.0	2.912617403358689	0.0
27	4.5272672780892035E-5	0.0	0.0	2.984782043771431	0.0
28	4.5272672780892035E-5	0.0	0.0	3.056946684184173	0.0
29	4.5272672780892035E-5	0.0	0.0	3.1450473054157886	0.0
30	4.5272672780892035E-5	0.0	0.0	3.2591797134964176	0.0
31	4.5272672780892035E-5	0.0	0.0	3.3518981473516845	0.0
32	4.5272672780892035E-5	0.0	0.0	3.449687120558411	0.0
33	4.5272672780892035E-5	0.0	0.0	3.545076642107751	0.0
34	4.5272672780892035E-5	0.0	0.0	3.6487510627759936	0.0
35	4.5272672780892035E-5	0.0	0.0	3.760484019199235	0.0
36	4.5272672780892035E-5	0.0	0.0	3.8563715401491643	0.0
37	4.5272672780892035E-5	0.0	0.0	3.9612683229824914	0.0
38	4.5272672780892035E-5	0.0	0.0	4.069651101619947	0.0
39	4.5272672780892035E-5	0.0	0.0	4.209181479130656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.0346997E-4	45.000004	1
ACGTCGA	20	7.0346997E-4	45.000004	14
ATACGTC	20	7.0346997E-4	45.000004	14
CGTTTTT	8455	0.0	41.859848	1
CGTAAGG	295	0.0	41.18644	2
GCGTAAG	160	0.0	39.375004	1
ATGTCGC	35	6.2509043E-6	38.57143	11
CTAAGCG	65	9.094947E-12	38.076927	1
CGGTCTA	305	0.0	37.62295	31
TTCGAAT	55	2.752131E-9	36.818184	16
ATAGGGC	1155	0.0	36.62338	4
AGGGCGA	2620	0.0	36.498093	6
GTACGAG	285	0.0	36.31579	1
ACGGGTA	355	0.0	36.126762	5
TAACGCG	25	0.0021075278	36.000004	1
CACTACG	25	0.0021075278	36.000004	17
CGTCGAA	25	0.0021075278	36.000004	15
GCCGATT	225	0.0	36.0	9
GGGCGAT	4890	0.0	35.93558	7
GTAGGGT	1085	0.0	35.6682	4
>>END_MODULE