FastQCFastQC Report
Thu 26 May 2016
SRR1546308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546308_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2081687
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123120.5914433822183642No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28670.13772483567414312No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT23990.1152430696833866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90050.042.7262651
TATCGTG1050.038.571431
TGATTCG4150.038.49397715
AAGGGCG9300.037.983875
AGGGCGA24950.037.6052136
ACCCGCT10850.037.3271934
TAAGGGA16850.037.1216624
GTTTTTT110650.036.9475822
GGGCGAT46250.036.7783787
TCACGAC2900.036.46551525
AATACGG1300.036.3461572
AATGGGC7150.036.1888124
CGAATAT2800.036.16071714
CATACGA6600.036.13636418
CGACGGT2750.036.028
ACCGTAT250.002107500336.028
CATATGC9450.035.9523833
TAGGGAC17000.035.7352945
CGGTCTA2900.035.68965531
TATGGGC10800.035.6254
Produced by FastQC (version 0.11.5)