##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546308_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2081687
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07482537000039	31.0	31.0	33.0	30.0	34.0
2	31.406290667136798	31.0	31.0	34.0	30.0	34.0
3	31.49630612094902	31.0	31.0	34.0	30.0	34.0
4	35.37874714114081	37.0	35.0	37.0	33.0	37.0
5	35.29537245512894	37.0	35.0	37.0	33.0	37.0
6	35.32606294798401	37.0	35.0	37.0	32.0	37.0
7	35.70885248358663	37.0	35.0	37.0	35.0	37.0
8	35.595421886191346	37.0	35.0	37.0	35.0	37.0
9	37.32910519208699	39.0	37.0	39.0	35.0	39.0
10	36.8236300654229	39.0	37.0	39.0	32.0	39.0
11	36.58553999712733	39.0	35.0	39.0	32.0	39.0
12	36.14751160957435	38.0	35.0	39.0	32.0	39.0
13	35.955708519100135	38.0	35.0	39.0	31.0	39.0
14	36.91636975203285	39.0	35.0	41.0	31.0	41.0
15	37.09362886927766	39.0	35.0	41.0	32.0	41.0
16	37.12202987288675	39.0	35.0	41.0	32.0	41.0
17	37.05048933869501	39.0	35.0	41.0	32.0	41.0
18	36.97700470820061	39.0	35.0	40.0	31.0	41.0
19	36.86492205600554	38.0	35.0	40.0	31.0	41.0
20	36.71255428890126	38.0	35.0	40.0	31.0	41.0
21	36.57121171434515	38.0	35.0	40.0	31.0	41.0
22	36.467859481276484	38.0	35.0	40.0	30.0	41.0
23	36.35879553458325	38.0	35.0	40.0	30.0	41.0
24	36.34627492029301	38.0	35.0	40.0	30.0	41.0
25	36.27371261865977	38.0	35.0	40.0	30.0	41.0
26	36.16602255766597	38.0	35.0	40.0	30.0	41.0
27	36.038910748830155	38.0	35.0	40.0	30.0	41.0
28	35.95260670792487	38.0	35.0	40.0	30.0	41.0
29	35.82243728283839	38.0	34.0	40.0	29.0	41.0
30	35.65656508399197	38.0	34.0	40.0	29.0	41.0
31	35.47624883087611	38.0	34.0	40.0	28.0	41.0
32	35.30298695240927	38.0	34.0	40.0	27.0	41.0
33	35.090021698747215	38.0	34.0	40.0	26.0	41.0
34	34.84263100072201	37.0	34.0	40.0	25.0	41.0
35	34.63895484767883	37.0	33.0	40.0	24.0	41.0
36	34.438129747651786	37.0	33.0	40.0	23.0	41.0
37	34.24604707624153	37.0	33.0	40.0	23.0	41.0
38	34.122326747488934	37.0	33.0	40.0	23.0	41.0
39	34.03221810003137	37.0	33.0	40.0	23.0	41.0
40	33.80752533882375	36.0	33.0	40.0	21.0	41.0
41	33.653698178448536	36.0	32.0	40.0	21.0	41.0
42	33.527637920590365	36.0	32.0	40.0	21.0	41.0
43	33.51017516081909	36.0	32.0	40.0	21.0	41.0
44	33.312495586512284	35.0	32.0	40.0	20.0	41.0
45	33.265362660188586	35.0	32.0	40.0	20.0	41.0
46	33.173705268851656	35.0	32.0	39.0	20.0	41.0
47	33.0834957416749	35.0	32.0	39.0	20.0	41.0
48	32.982136603629655	35.0	31.0	39.0	20.0	41.0
49	32.95429284037418	35.0	31.0	39.0	20.0	41.0
50	32.80949585600525	35.0	31.0	39.0	20.0	41.0
51	32.58304106236913	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	3.0
11	4.0
12	12.0
13	15.0
14	35.0
15	105.0
16	304.0
17	699.0
18	1588.0
19	2977.0
20	5262.0
21	8247.0
22	11952.0
23	16934.0
24	23568.0
25	31895.0
26	40780.0
27	47045.0
28	50463.0
29	55073.0
30	63834.0
31	75517.0
32	91082.0
33	114602.0
34	174399.0
35	215115.0
36	172284.0
37	218236.0
38	297036.0
39	362560.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.81406522690491	23.094250000120095	28.138091845700146	14.953592927274851
2	32.21166294452528	25.1505149429285	29.40432447337184	13.233497639174383
3	29.67280864029991	25.02532801521074	31.943274853520247	13.358588490969103
4	26.254907678243654	27.43049267252954	30.796752825953185	15.517846823273624
5	23.250133185248313	31.536393319456767	30.214965074000077	14.998508421294844
6	20.71430527259862	40.293617628394664	27.804660354798777	11.187416744207942
7	85.69717733741912	4.326346852336591	7.907048465979756	2.0694273442645317
8	86.40698625681958	3.694263354673397	7.474178394734656	2.4245719937723584
9	81.37452940811947	5.165666115991501	9.81694174004065	3.642862735848377
10	43.241178909221226	24.580688643393557	16.707362826399937	15.470769620985287
11	35.846743530607625	23.74780646658215	23.317050065643873	17.088399937166347
12	32.36812258519172	21.299743909627146	28.15279146192487	18.179342043256263
13	24.567670355821985	27.625959137949174	28.3289947047755	19.477375801453338
14	20.601848404683317	30.848874014200984	29.43996864081872	19.10930894029698
15	19.24093295485825	23.116443538341738	37.8654427875084	19.77718071929161
16	21.317565993350584	19.814410139468613	36.70282804283257	22.16519582434823
17	21.404370589814896	21.144581293921707	30.00768127004684	27.443366846216556
18	24.325510991806166	22.913963530540375	30.79977921752886	21.9607462601246
19	27.924178803057327	24.74449809217236	25.88631239951059	21.445010705259723
20	29.935624327768778	23.069510449937958	25.29323572660059	21.701629495692675
21	26.103828289267312	25.39051259867598	27.155379266911883	21.350279845144826
22	24.762416251818838	23.119229740109827	26.48087824922767	25.63747575884367
23	23.710048628828446	26.60087707710141	25.624553547195134	24.064520746875008
24	23.02132837453469	24.608838888843522	31.355194128608193	21.0146386080136
25	23.227315153526924	24.30903397100525	29.332892024593516	23.130758850874315
26	22.065420978273874	27.353055478561377	26.750179061501562	23.831344481663187
27	22.41830784359032	26.28219324038628	28.241373462965374	23.05812545305802
28	21.39159249205092	26.16498061428063	31.10116938809725	21.3422575055712
29	22.135316212283595	24.983823216458575	30.247919115601913	22.632941455655917
30	23.795364048485677	25.265517822804295	29.147801758861924	21.79131636984811
31	27.28215144735976	24.826594968407832	25.48072789040812	22.410525693824287
32	27.77737479265615	24.29673625285646	25.567148183180276	22.35874077130712
33	26.807632463477937	25.072165027691483	24.944384050051713	23.175818458778867
34	23.512228303294393	25.491824659518937	27.402390465041094	23.593556572145573
35	23.471972491541713	24.797291811881422	27.72366835167823	24.007067344898633
36	27.281623029783052	26.220944839449928	24.611480976727048	21.88595115403997
37	24.150844963724133	27.95737303446676	26.222914395872195	21.668867605936914
38	24.577374024048765	28.189924806178833	24.295295114010894	22.937406055761507
39	23.861608397419978	27.247420001181734	24.913735830602775	23.977235770795513
40	25.221418974130117	25.22473359347491	26.266388751046627	23.28745868134835
41	22.258773773386682	24.090509284056633	26.867775991299364	26.782940951257324
42	23.54369316808915	26.1125231603022	24.975080307462168	25.368703364146484
43	23.63020953678435	25.65198322322232	26.09859215146177	24.61921508853156
44	23.854690930961283	26.291656718805466	26.787648671486153	23.066003678747094
45	22.037222694862386	28.050662755736095	25.70251915874	24.209595390661516
46	23.499210015722824	27.66780020243197	26.3710634691959	22.461926312649307
47	23.35149328405279	26.498219953335923	27.073282390676408	23.07700437193488
48	23.644476811355407	24.84528173543861	27.76733485869874	23.742906594507243
49	22.996732938237113	24.83706724401891	28.044754086469293	24.121445731274683
50	22.28750047437487	26.764302222188064	27.06060036883547	23.8875969346016
51	22.455008846190612	27.854331607009126	25.745320982453173	23.94533856434709
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	747.0
1	1626.5
2	2506.0
3	12530.0
4	22554.0
5	16342.5
6	10131.0
7	10008.0
8	9885.0
9	10021.5
10	10158.0
11	10005.5
12	9853.0
13	9682.0
14	9511.0
15	9129.5
16	8748.0
17	8408.5
18	8069.0
19	7874.5
20	7680.0
21	7695.0
22	7710.0
23	8507.0
24	9304.0
25	9948.0
26	13085.5
27	15579.0
28	18029.0
29	20479.0
30	23055.0
31	25631.0
32	29762.0
33	33893.0
34	38864.5
35	43836.0
36	48269.5
37	52703.0
38	58163.0
39	63623.0
40	76804.0
41	89985.0
42	104370.5
43	118756.0
44	124086.5
45	129417.0
46	131602.0
47	133787.0
48	140624.0
49	147461.0
50	152464.5
51	157468.0
52	151779.5
53	146091.0
54	137428.0
55	128765.0
56	128193.0
57	127621.0
58	123145.0
59	118669.0
60	113051.5
61	107434.0
62	95874.5
63	84315.0
64	75234.5
65	66154.0
66	57064.5
67	47975.0
68	40694.0
69	33413.0
70	28316.0
71	23219.0
72	19763.0
73	16307.0
74	13500.0
75	8205.0
76	5717.0
77	4194.0
78	2671.0
79	2000.5
80	1330.0
81	921.5
82	513.0
83	425.0
84	337.0
85	256.0
86	175.0
87	148.0
88	121.0
89	100.5
90	80.0
91	46.5
92	13.0
93	9.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2081687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.598462666243066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85158395607567	17.593261135392634
2	5.86121890054791	2.6490907300542035
3	2.1946881357264973	1.4878973369778554
4	1.3632718021473191	1.232313876990724
5	0.9856451016354159	1.1137032015736645
6	0.7622806220440385	1.0335822107077646
7	0.6561270412025967	1.0379223711450587
8	0.548929559003676	0.9923971316433475
9	0.4659152306187461	0.9476071150294587
>10	7.529070084548244	41.36381979146439
>50	1.6436521795126546	24.66154785445583
>100	0.13246479509482822	4.243637026460754
>500	0.003649752555116102	0.5422738308704792
>1k	0.0012881479606292126	0.5036058302259618
>5k	0.0	0.0
>10k+	2.146913267715354E-4	0.5973405570079038
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12312	0.5914433822183642	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2867	0.13772483567414312	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2399	0.1152430696833866	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8037961518710546E-5	0.0	0.0	0.020704361414564245	0.0
2	4.8037961518710546E-5	0.0	0.0	0.07138441081680387	0.0
3	4.8037961518710546E-5	0.0	0.0	0.1138980067608627	0.0
4	4.8037961518710546E-5	0.0	0.0	0.1841295065012175	0.0
5	4.8037961518710546E-5	0.0	0.0	0.33285503536314537	0.0
6	4.8037961518710546E-5	0.0	0.0	0.637463749353289	0.0
7	4.8037961518710546E-5	0.0	0.0	0.8485425522665031	0.0
8	4.8037961518710546E-5	0.0	0.0	1.454637512748074	0.0
9	4.8037961518710546E-5	0.0	0.0	1.8118958325627244	0.0
10	4.8037961518710546E-5	0.0	0.0	2.276951338025361	0.0
11	4.8037961518710546E-5	0.0	0.0	2.532225065535789	0.0
12	4.8037961518710546E-5	0.0	0.0	2.7494046895618793	0.0
13	4.8037961518710546E-5	0.0	0.0	2.840196436832242	0.0
14	4.8037961518710546E-5	0.0	0.0	2.8829982605454134	0.0
15	4.8037961518710546E-5	0.0	0.0	2.925031476874285	0.0
16	4.8037961518710546E-5	0.0	0.0	3.008857719724435	0.0
17	4.8037961518710546E-5	0.0	0.0	3.1067110473380484	0.0
18	4.8037961518710546E-5	0.0	0.0	3.2468858190496457	0.0
19	4.8037961518710546E-5	0.0	0.0	3.3144752309064716	4.8037961518710546E-5
20	4.8037961518710546E-5	0.0	0.0	3.389558564760216	4.8037961518710546E-5
21	4.8037961518710546E-5	0.0	0.0	3.4810708814533595	4.8037961518710546E-5
22	4.8037961518710546E-5	0.0	0.0	3.576089969337369	4.8037961518710546E-5
23	4.8037961518710546E-5	0.0	0.0	3.6829263957549814	4.8037961518710546E-5
24	4.8037961518710546E-5	0.0	0.0	3.766320296951463	4.8037961518710546E-5
25	4.8037961518710546E-5	0.0	0.0	3.836503758730299	4.8037961518710546E-5
26	4.8037961518710546E-5	0.0	0.0	3.9042853224331995	4.8037961518710546E-5
27	4.8037961518710546E-5	0.0	0.0	3.975717771211522	4.8037961518710546E-5
28	4.8037961518710546E-5	0.0	0.0	4.052722623526015	4.8037961518710546E-5
29	4.8037961518710546E-5	0.0	0.0	4.1409203208743675	4.8037961518710546E-5
30	4.8037961518710546E-5	0.0	0.0	4.266587628207315	4.8037961518710546E-5
31	4.8037961518710546E-5	0.0	0.0	4.362999816975367	4.8037961518710546E-5
32	4.8037961518710546E-5	0.0	0.0	4.462822701011247	4.8037961518710546E-5
33	4.8037961518710546E-5	0.0	0.0	4.5624053952395345	4.8037961518710546E-5
34	4.8037961518710546E-5	0.0	0.0	4.665590936581724	4.8037961518710546E-5
35	4.8037961518710546E-5	0.0	0.0	4.785109384840276	4.8037961518710546E-5
36	4.8037961518710546E-5	0.0	0.0	4.888391002105504	4.8037961518710546E-5
37	4.8037961518710546E-5	0.0	0.0	4.993834327639074	4.8037961518710546E-5
38	4.8037961518710546E-5	0.0	0.0	5.120126128471764	4.8037961518710546E-5
39	4.8037961518710546E-5	0.0	0.0	5.313142657853943	4.8037961518710546E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9005	0.0	42.726265	1
TATCGTG	105	0.0	38.57143	1
TGATTCG	415	0.0	38.493977	15
AAGGGCG	930	0.0	37.98387	5
AGGGCGA	2495	0.0	37.605213	6
ACCCGCT	1085	0.0	37.32719	34
TAAGGGA	1685	0.0	37.121662	4
GTTTTTT	11065	0.0	36.947582	2
GGGCGAT	4625	0.0	36.778378	7
TCACGAC	290	0.0	36.465515	25
AATACGG	130	0.0	36.346157	2
AATGGGC	715	0.0	36.188812	4
CGAATAT	280	0.0	36.160717	14
CATACGA	660	0.0	36.136364	18
CGACGGT	275	0.0	36.0	28
ACCGTAT	25	0.0021075003	36.0	28
CATATGC	945	0.0	35.95238	33
TAGGGAC	1700	0.0	35.735294	5
CGGTCTA	290	0.0	35.689655	31
TATGGGC	1080	0.0	35.625	4
>>END_MODULE