FastQCFastQC Report
Thu 26 May 2016
SRR1546307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546307_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1392133
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.4689925459708232No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52460.3768318113283716No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15460.11105260776089641No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC253.891231E-545.00000421
CGGCGTA253.891231E-545.00000434
GTACCGT207.0338E-445.00000415
GATCGCG207.0338E-445.00000410
CATCTCG406.8175723E-945.00000424
TTCGTAC207.0338E-445.00000433
ATTCGAA253.891231E-545.00000440
TATTCGC253.891231E-545.00000412
TCGTCAG207.0338E-445.00000440
TTGCGCA650.045.0000041
AGCGAAT207.0338E-445.00000429
CGTACAC253.891231E-545.0000048
CCACGCG207.0338E-445.0000041
GTAACGA207.0338E-445.00000417
GCGTACC207.0338E-445.00000413
TACGTTA207.0338E-445.00000442
CTTACGT253.891231E-545.00000424
CGGGTCA351.2121745E-745.06
CTATGCG453.8562575E-1045.01
AAATGCG351.2121745E-745.01