##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546307_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1392133
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.155860108193686	31.0	31.0	33.0	30.0	34.0
2	31.489437431624708	31.0	31.0	34.0	30.0	34.0
3	31.547678993314577	31.0	31.0	34.0	30.0	34.0
4	35.38544018423527	37.0	35.0	37.0	33.0	37.0
5	35.277749324238414	37.0	35.0	37.0	33.0	37.0
6	35.30637302614046	37.0	35.0	37.0	32.0	37.0
7	35.78280954477769	37.0	35.0	37.0	35.0	37.0
8	35.70668966255379	37.0	35.0	37.0	35.0	37.0
9	37.4694975264576	39.0	37.0	39.0	35.0	39.0
10	36.96136647863386	39.0	37.0	39.0	32.0	39.0
11	36.57748936344444	39.0	35.0	39.0	32.0	39.0
12	35.42670420139455	37.0	35.0	39.0	31.0	39.0
13	34.845113218349105	37.0	34.0	39.0	29.0	39.0
14	35.658501019658324	37.0	34.0	40.0	28.0	41.0
15	36.128703938488634	37.0	35.0	40.0	31.0	41.0
16	36.35112018751082	37.0	35.0	40.0	32.0	41.0
17	36.32905476703734	37.0	35.0	40.0	32.0	41.0
18	36.25845806399245	36.0	35.0	40.0	31.0	41.0
19	36.12282662647893	36.0	35.0	40.0	31.0	41.0
20	35.92629224362902	36.0	34.0	40.0	31.0	41.0
21	35.72701099679413	35.0	34.0	40.0	30.0	41.0
22	35.58658044885079	35.0	34.0	40.0	30.0	41.0
23	35.57480571181058	35.0	34.0	40.0	30.0	41.0
24	35.52312889644883	35.0	34.0	40.0	30.0	41.0
25	35.47766053961798	35.0	34.0	40.0	30.0	41.0
26	35.33494141723528	35.0	34.0	40.0	30.0	41.0
27	35.201160377636334	35.0	34.0	40.0	29.0	41.0
28	35.21268585688293	35.0	34.0	40.0	29.0	41.0
29	35.22831008244184	36.0	34.0	40.0	29.0	41.0
30	35.12270451170973	36.0	34.0	40.0	29.0	41.0
31	34.84086721599157	35.0	34.0	40.0	29.0	41.0
32	34.600451249988325	35.0	34.0	40.0	27.0	41.0
33	34.38901168207348	35.0	33.0	40.0	26.0	41.0
34	34.20523326435046	35.0	34.0	40.0	25.0	41.0
35	34.00599583516805	35.0	33.0	40.0	24.0	41.0
36	33.72673444275799	35.0	33.0	40.0	23.0	41.0
37	33.550665776904935	35.0	33.0	39.0	23.0	41.0
38	33.54456147508895	35.0	33.0	39.0	23.0	41.0
39	33.546457845622506	35.0	33.0	39.0	23.0	41.0
40	33.285067590524754	35.0	32.0	39.0	22.0	41.0
41	33.28629161150551	35.0	32.0	39.0	22.0	41.0
42	33.17891322165339	35.0	32.0	39.0	22.0	41.0
43	33.11074732083788	35.0	32.0	39.0	22.0	41.0
44	32.97890359613629	35.0	32.0	39.0	21.0	41.0
45	32.9397313331413	35.0	32.0	39.0	22.0	41.0
46	32.87119693305166	35.0	32.0	38.0	21.0	40.0
47	32.827016527874854	35.0	32.0	38.0	21.0	40.0
48	32.790981896126304	35.0	32.0	38.0	20.0	40.0
49	32.84981822857443	35.0	32.0	38.0	20.0	40.0
50	32.656593155970015	35.0	32.0	38.0	20.0	40.0
51	32.40768734021821	35.0	31.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	1.0
11	3.0
12	1.0
13	5.0
14	25.0
15	67.0
16	175.0
17	481.0
18	1059.0
19	2048.0
20	3526.0
21	5492.0
22	8110.0
23	11722.0
24	16302.0
25	22583.0
26	29236.0
27	33605.0
28	35283.0
29	38145.0
30	44898.0
31	54074.0
32	68199.0
33	88801.0
34	161775.0
35	233422.0
36	88258.0
37	102118.0
38	140318.0
39	202367.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.145371886163176	23.51298331409427	30.281014816831437	15.060629982911117
2	29.682580615501536	26.13069297258236	30.52481336194171	13.661913049974391
3	27.51202650896143	24.932028764493047	33.72774009379851	13.828204632747015
4	25.09113712554763	27.787718558499797	32.26193187001529	14.859212445937278
5	23.296624675946912	31.296219542242014	31.37264902132196	14.034506760489121
6	21.51080392462502	39.14776820892831	28.51056615998615	10.830861706460517
7	84.96601976966282	5.130472447675618	7.948162998793936	1.9553447838676334
8	87.18843673700717	3.226272202440428	7.275454284899503	2.3098367756529012
9	83.26912730321025	4.667801136816669	9.297172037441825	2.7658995225312526
10	57.266798502729266	20.07344125884524	12.923980682880156	9.735779555545339
11	52.097249328907516	16.89306984318309	20.37125763127517	10.63842319663423
12	46.60287486899599	20.38871286005001	21.776798624844034	11.231613646109963
13	20.380236658422724	45.44522685691669	21.722062475352573	12.45247400930802
14	12.717606722920872	47.434045453990386	26.859071654791606	12.989276168297136
15	11.233265787105111	22.01578441140322	53.83120721942516	12.919742582066512
16	12.592475000592614	15.480058299027464	52.48018687869621	19.447279821683704
17	14.355955932371403	16.59259567871748	29.331321073489384	39.720127315421735
18	20.524906743824044	21.621712867951555	36.61568255331925	21.23769783490514
19	29.20949363315143	22.8623989230914	25.48370019243851	22.44440725131866
20	32.019282640379906	21.014658800560003	25.59123302155757	21.374825537502524
21	21.384522886821877	28.70882307940405	27.96629345041027	21.940360583363802
22	23.06000935255468	24.56676194013072	24.324400039364054	28.04882866795055
23	18.446226043057667	31.641948003531272	23.853324359094998	26.05850159431606
24	19.1728089198374	22.553233060346965	41.94031748403349	16.33364053578214
25	15.033046411513842	24.908108636171974	38.97687936425614	21.08196558805804
26	15.206664880438867	34.447283413294564	29.19002710229554	21.15602460397103
27	16.18782113490593	35.552350242397814	28.700346877776767	19.559481744919488
28	13.23623533096335	28.01643233800219	41.2029597746767	17.54437255635776
29	13.376092657813585	22.356412785272674	40.910027993015035	23.357466563898708
30	17.70613870944802	30.1693875513331	32.39051153876821	19.733962200450677
31	28.47249508488054	26.941894201200604	24.690026024812283	19.89558468910657
32	30.042460023575334	26.12135478434891	26.041764687713027	17.79442050436273
33	28.38723024308741	27.230587882048624	25.151404355761986	19.230777519101984
34	18.261329916035322	26.489782226267174	29.437201761613295	25.81168609608421
35	19.30605768270704	24.546002429365586	32.13529167112625	24.012648216801125
36	31.08697229359551	23.007428169578624	26.090251434309796	19.81534810251607
37	20.112158823905474	30.609503545997402	30.12442058337817	19.153917046718956
38	19.560343731525652	32.33010064411949	25.03173188193944	23.077823742415415
39	19.475007057515338	31.764781094909754	27.838791264915063	20.92142058265985
40	23.416081653117914	25.48018041379667	25.16778210127912	25.9359558318063
41	16.3220755488161	23.272058057671213	27.447090184630348	32.95877620888234
42	21.80653716275672	25.02957691542403	23.98951824286904	29.174367678950215
43	22.14177812033764	26.639336902436767	25.616015136484805	25.602869840740794
44	19.575787658219436	30.43976401680012	27.77198730293729	22.212461022043154
45	15.803087779687717	38.13816639645781	22.23889527796554	23.819850545888936
46	22.086970138628995	33.21169744557452	25.430616183942195	19.270716231854284
47	21.578541705426133	26.914957119757954	27.870181943822896	23.636319230993017
48	22.83912528472495	22.47852755447935	31.63390279520707	23.048444365588633
49	20.543295791422228	22.225318988918445	32.94656473196167	24.284820487697655
50	18.59039330293873	32.96251148417572	26.87623955469772	21.57085565818783
51	17.096570514455156	34.97539387400486	22.776200262474923	25.151835349065067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	816.0
1	1020.0
2	1224.0
3	5877.0
4	10530.0
5	8060.0
6	5590.0
7	6093.5
8	6597.0
9	7100.0
10	7603.0
11	7734.0
12	7865.0
13	7629.5
14	7394.0
15	6967.0
16	6540.0
17	6369.0
18	6198.0
19	5648.5
20	5099.0
21	4948.5
22	4798.0
23	4754.5
24	4711.0
25	5406.5
26	6291.5
27	6481.0
28	8462.5
29	10444.0
30	13336.0
31	16228.0
32	21394.0
33	26560.0
34	30114.0
35	33668.0
36	36371.5
37	39075.0
38	46318.0
39	53561.0
40	79445.5
41	105330.0
42	128860.5
43	152391.0
44	151724.0
45	151057.0
46	142539.5
47	134022.0
48	126230.5
49	118439.0
50	114521.5
51	110604.0
52	96575.0
53	82546.0
54	72462.5
55	62379.0
56	56175.0
57	49971.0
58	46196.5
59	42422.0
60	38558.5
61	34695.0
62	31846.0
63	28997.0
64	23679.0
65	18361.0
66	15176.0
67	11991.0
68	10357.0
69	8723.0
70	6981.0
71	5239.0
72	4483.5
73	3728.0
74	2779.5
75	1400.5
76	970.0
77	827.5
78	685.0
79	479.5
80	274.0
81	195.5
82	117.0
83	149.5
84	182.0
85	117.0
86	52.0
87	34.5
88	17.0
89	12.5
90	8.0
91	7.5
92	7.0
93	8.5
94	10.0
95	5.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1392133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.78496397531696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.68722313703437	16.161787670099027
2	7.511796524559664	2.9724124725664813
3	2.352691966405637	1.3964377740105944
4	1.0806648833335808	0.8552366314457999
5	0.674584537657632	0.6673315387931048
6	0.4480129183963456	0.5318351670568988
7	0.3177347776252352	0.4400459790314437
8	0.25378313288263576	0.40168721133008195
9	0.2149266002270595	0.382708353854675
>10	2.6384927639196736	12.628042484741542
>50	1.3500541314521697	19.70384841733645
>100	1.4660231204549568	42.49336094864507
>500	0.002552776577906641	0.32850930892651375
>1k	7.293647365447547E-4	0.18716300183513657
>5k	7.293647365447547E-4	0.8495930403271907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6529	0.4689925459708232	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5246	0.3768318113283716	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1546	0.11105260776089641	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07686047238302662	0.0
2	0.0	0.0	0.0	0.3180012254576251	0.0
3	0.0	0.0	0.0	0.44370760552332283	0.0
4	0.0	0.0	0.0	0.6475674378812943	0.0
5	0.0	0.0	0.0	1.0907722178843544	0.0
6	0.0	0.0	0.0	1.5006468491157094	0.0
7	0.0	0.0	0.0	1.7493299849942499	0.0
8	0.0	0.0	0.0	2.402069342512533	0.0
9	0.0	0.0	0.0	2.6488130085272026	0.0
10	0.0	0.0	0.0	3.0714737744166687	0.0
11	0.0	0.0	0.0	3.4753863316220506	0.0
12	0.0	0.0	0.0	3.813859738976089	0.0
13	0.0	0.0	0.0	3.95091560935629	0.0
14	0.0	0.0	0.0	3.99954602038742	0.0
15	0.0	0.0	0.0	4.089408124080099	0.0
16	0.0	0.0	0.0	4.3017441580653575	0.0
17	0.0	0.0	0.0	4.535055199467292	0.0
18	0.0	0.0	0.0	4.889978184555642	0.0
19	0.0	0.0	0.0	5.023945269597086	0.0
20	0.0	0.0	0.0	5.165598401876832	0.0
21	0.0	0.0	0.0	5.357462253965677	0.0
22	0.0	0.0	0.0	5.540849904427235	0.0
23	0.0	0.0	0.0	5.776890570082025	0.0
24	0.0	0.0	0.0	5.942032837379762	0.0
25	0.0	0.0	0.0	6.072192814910644	0.0
26	0.0	0.0	0.0	6.206231732169268	0.0
27	0.0	0.0	0.0	6.330214139022637	0.0
28	0.0	0.0	0.0	6.455274029133711	0.0
29	0.0	0.0	0.0	6.590749590735943	0.0
30	0.0	0.0	0.0	6.791305141103615	0.0
31	0.0	0.0	0.0	6.973040650570025	0.0
32	0.0	0.0	0.0	7.1216615079162695	0.0
33	0.0	0.0	0.0	7.260369519291619	0.0
34	0.0	0.0	0.0	7.419837041432104	0.0
35	0.0	0.0	0.0	7.645821196681639	0.0
36	0.0	0.0	0.0	7.816422712485086	0.0
37	0.0	0.0	0.0	7.979410013267411	0.0
38	0.0	0.0	0.0	8.128964689437	0.0
39	0.0	0.0	0.0	8.289006869314928	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	25	3.891231E-5	45.000004	21
CGGCGTA	25	3.891231E-5	45.000004	34
GTACCGT	20	7.0338E-4	45.000004	15
GATCGCG	20	7.0338E-4	45.000004	10
CATCTCG	40	6.8175723E-9	45.000004	24
TTCGTAC	20	7.0338E-4	45.000004	33
ATTCGAA	25	3.891231E-5	45.000004	40
TATTCGC	25	3.891231E-5	45.000004	12
TCGTCAG	20	7.0338E-4	45.000004	40
TTGCGCA	65	0.0	45.000004	1
AGCGAAT	20	7.0338E-4	45.000004	29
CGTACAC	25	3.891231E-5	45.000004	8
CCACGCG	20	7.0338E-4	45.000004	1
GTAACGA	20	7.0338E-4	45.000004	17
GCGTACC	20	7.0338E-4	45.000004	13
TACGTTA	20	7.0338E-4	45.000004	42
CTTACGT	25	3.891231E-5	45.000004	24
CGGGTCA	35	1.2121745E-7	45.0	6
CTATGCG	45	3.8562575E-10	45.0	1
AAATGCG	35	1.2121745E-7	45.0	1
>>END_MODULE