##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546306_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1058929
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26139335120674	31.0	31.0	33.0	30.0	34.0
2	31.59700980896736	31.0	31.0	34.0	30.0	34.0
3	31.661926342559322	31.0	31.0	34.0	30.0	34.0
4	35.49168168970724	37.0	35.0	37.0	33.0	37.0
5	35.41708934215608	37.0	35.0	37.0	33.0	37.0
6	35.46880763488392	37.0	35.0	37.0	33.0	37.0
7	35.857726060954036	37.0	35.0	37.0	35.0	37.0
8	35.77982565403346	37.0	35.0	37.0	35.0	37.0
9	37.5447541808752	39.0	37.0	39.0	35.0	39.0
10	37.12031212668649	39.0	37.0	39.0	33.0	39.0
11	36.76295200150341	39.0	35.0	39.0	32.0	39.0
12	35.67721443080698	37.0	35.0	39.0	31.0	39.0
13	35.15802475897817	37.0	35.0	39.0	30.0	39.0
14	36.039632496607425	38.0	35.0	40.0	30.0	41.0
15	36.43767145861526	38.0	35.0	40.0	31.0	41.0
16	36.64504419087588	38.0	35.0	40.0	32.0	41.0
17	36.61374747504318	38.0	35.0	40.0	32.0	41.0
18	36.53687168828127	37.0	35.0	40.0	32.0	41.0
19	36.37982527629331	37.0	35.0	40.0	31.0	41.0
20	36.20882703184066	36.0	35.0	40.0	31.0	41.0
21	36.04434102758542	36.0	35.0	40.0	31.0	41.0
22	35.92596198611994	36.0	34.0	40.0	31.0	41.0
23	35.91158141858425	36.0	34.0	40.0	31.0	41.0
24	35.8476677850923	35.0	34.0	40.0	31.0	41.0
25	35.79563124628752	35.0	34.0	40.0	31.0	41.0
26	35.67089200503528	35.0	34.0	40.0	31.0	41.0
27	35.56428995711705	35.0	34.0	40.0	30.0	41.0
28	35.597285559277346	36.0	34.0	40.0	30.0	41.0
29	35.595386470669894	36.0	34.0	40.0	30.0	41.0
30	35.48511939893987	36.0	34.0	40.0	30.0	41.0
31	35.24398236331236	35.0	34.0	40.0	30.0	41.0
32	35.008150688100905	35.0	34.0	40.0	29.0	41.0
33	34.802824363106495	35.0	34.0	40.0	27.0	41.0
34	34.609404407660946	35.0	34.0	40.0	27.0	41.0
35	34.39133596303435	35.0	34.0	40.0	25.0	41.0
36	34.13956459781534	35.0	33.0	40.0	23.0	41.0
37	33.923563336163234	35.0	33.0	40.0	23.0	41.0
38	33.90983909213932	35.0	33.0	40.0	23.0	41.0
39	33.91675740299869	35.0	33.0	40.0	23.0	41.0
40	33.707291045953035	35.0	33.0	40.0	23.0	41.0
41	33.6832809376266	35.0	33.0	40.0	23.0	41.0
42	33.580851974022806	35.0	33.0	39.0	23.0	41.0
43	33.508389136571004	35.0	33.0	39.0	23.0	41.0
44	33.34836707654621	35.0	33.0	39.0	23.0	41.0
45	33.29718706353306	35.0	33.0	39.0	23.0	41.0
46	33.22911167793119	35.0	33.0	39.0	23.0	41.0
47	33.213141768711594	35.0	32.0	39.0	23.0	41.0
48	33.19623128651685	35.0	33.0	39.0	23.0	41.0
49	33.24476617412499	35.0	33.0	39.0	23.0	41.0
50	33.07212759306809	35.0	32.0	39.0	22.0	41.0
51	32.847216385612256	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	4.0
13	5.0
14	14.0
15	35.0
16	85.0
17	261.0
18	560.0
19	1198.0
20	2013.0
21	3350.0
22	5060.0
23	7542.0
24	10772.0
25	15749.0
26	20276.0
27	22769.0
28	23927.0
29	26574.0
30	30996.0
31	38388.0
32	48635.0
33	64618.0
34	120373.0
35	175970.0
36	70128.0
37	82098.0
38	118330.0
39	169166.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.5302810670026	23.827659833662125	29.928352136923248	15.713706962412022
2	29.685937395236127	26.246991063612384	31.16818974643248	12.898881794719003
3	27.50939864712365	24.699011926200907	33.71812463347401	14.073464793201431
4	24.38935943769601	27.555010770316045	32.92213170099223	15.133498090995715
5	21.939903430730485	32.69048255359897	31.481714071481658	13.887899944188892
6	20.417893928676996	39.378938531289634	29.16673355815168	11.036433981881693
7	86.09198539278837	4.56952260255409	7.5876664063407455	1.7508255983167897
8	87.95471651073868	3.559634309760144	6.769481240007592	1.7161679394935827
9	83.55602689132132	4.591337096254801	9.167092411294808	2.685543601129065
10	56.55261117600897	20.386730366247406	12.75005217535831	10.310606282385315
11	50.10345358376246	18.170528902315453	20.13770517192371	11.588312341998378
12	44.65351312505371	20.431870314251473	22.72173110756245	12.192885453132362
13	20.527627442444203	44.27303435829975	22.60689810176131	12.592440097494734
14	14.088479964190235	44.818207830742196	27.08925716455022	14.004055040517352
15	11.54789414587758	21.166102732100075	53.35494636562036	13.931056756401988
16	12.851475405810966	16.284094589911128	50.366549598698306	20.4978804055796
17	14.111049938192268	16.89735572451033	29.590746877269392	39.40084746002801
18	21.164780641572758	21.6646252959358	35.85282865990071	21.31776540259073
19	28.716845038713647	23.290041164232917	25.658660778956854	22.334453018096585
20	31.694476211341836	21.796928783705045	24.864273242115384	21.644321762837734
21	21.003957772428556	27.760312542200655	27.798275427342155	23.43745425802863
22	22.623046493202093	25.189601946872735	24.634135055324766	27.553216504600403
23	18.52815438995438	31.314280749700877	24.465568513092002	25.691996347252744
24	20.126656272516854	23.035255432611628	40.538600793820926	16.29948750105059
25	15.227177648359804	26.235092248866543	36.59272718000924	21.945002922764413
26	15.034624606560024	35.558285777422284	28.334855311357042	21.072234304660654
27	15.882556809757784	33.590259592475036	29.96518180161276	20.562001796154416
28	13.663805599808864	27.608933176822998	41.13033073983242	17.596930483535722
29	13.767684141240819	23.261710652933292	40.80641856064005	22.164186645185843
30	17.33893396063381	29.688109401102437	33.67440121103492	19.298555427228834
31	28.702868653139163	25.962552730164155	25.048988175788935	20.285590440907747
32	30.208918633827196	26.82672775984037	24.814411542228044	18.149942064104394
33	27.11050504802494	27.969297280554223	24.92905567795386	19.991141993466986
34	18.22142938761711	26.890093670113863	30.5734378792157	24.31503906305333
35	20.45189054223654	25.13227987900983	31.75642559605035	22.659403982703278
36	30.736999364452195	22.97651683918374	26.535773408793222	19.750710387570837
37	20.702898872351216	30.279744912076257	29.812102605557122	19.2052536100154
38	20.53905408200172	32.16570704929226	24.952097827144218	22.343141041561804
39	20.12656183747919	30.855232031609297	28.04937819249449	20.96882793841702
40	24.019362960122915	25.552893536771588	24.811484056060415	25.61625944704508
41	17.270374123288722	22.07919511128697	28.245614200763224	32.40481656466109
42	22.09421028227577	25.5195579684757	23.609514896655018	28.776716852593516
43	22.14841599389572	27.03269057698864	25.65167258616961	25.16722084294603
44	20.748605430581275	31.20303627533102	27.05837690723363	20.989981386854076
45	17.05534554252457	37.256227754646446	21.87096585323473	23.81746084959426
46	21.76076016427919	33.023177191294224	26.10581068230259	19.110251962123996
47	21.674163234740007	26.919651836903135	28.360258336489036	23.045926591867822
48	23.781292230168404	22.63626739847525	30.34972127498633	23.232719096370012
49	19.706514789943423	22.617663696055164	32.53881988310831	25.137001630893103
50	18.30689309670431	32.514833383541294	27.548683622792463	21.62958989696193
51	17.279345451866934	34.31929808325204	23.96695151421861	24.434404950662415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	524.0
1	714.5
2	905.0
3	4536.0
4	8167.0
5	6203.5
6	4240.0
7	4632.5
8	5025.0
9	5488.0
10	5951.0
11	6069.5
12	6188.0
13	5982.5
14	5777.0
15	5620.5
16	5464.0
17	4964.0
18	4464.0
19	4237.0
20	4010.0
21	3824.5
22	3639.0
23	3705.5
24	3772.0
25	4191.5
26	5417.0
27	6223.0
28	7762.5
29	9302.0
30	10635.5
31	11969.0
32	15493.5
33	19018.0
34	23561.5
35	28105.0
36	28061.5
37	28018.0
38	35961.5
39	43905.0
40	60301.0
41	76697.0
42	95197.5
43	113698.0
44	109853.5
45	106009.0
46	99425.5
47	92842.0
48	89571.5
49	86301.0
50	86533.5
51	86766.0
52	80280.0
53	73794.0
54	64291.0
55	54788.0
56	47839.0
57	40890.0
58	36411.5
59	31933.0
60	29371.5
61	26810.0
62	23847.0
63	20884.0
64	17632.0
65	14380.0
66	11469.0
67	8558.0
68	6690.5
69	4823.0
70	4330.5
71	3838.0
72	3372.5
73	2907.0
74	2376.0
75	1407.0
76	969.0
77	714.5
78	460.0
79	377.5
80	295.0
81	196.0
82	97.0
83	63.5
84	30.0
85	23.0
86	16.0
87	12.0
88	8.0
89	9.5
90	11.0
91	6.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1058929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.591558893486866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.59525083591438	15.985781621806503
2	6.462536838831891	2.5322234215860684
3	2.0360557836354873	1.1966852038655762
4	1.0555679204568857	0.8272088431882615
5	0.7021208788766098	0.6877821274428929
6	0.5054191103353138	0.5941168959616822
7	0.4079648391263211	0.559486702055065
8	0.32328906598951096	0.5066989420763093
9	0.2556830831236522	0.45083071629767957
>10	3.490099008259772	17.164757650388147
>50	2.05078976921574	29.95669066999403
>100	1.1137701225963073	28.368917756850674
>500	0.0	0.0
>1k	0.0014527436381690964	1.168819448487079
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4922	0.4648092553891715	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4759	0.4494163442497089	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2639	0.2492140643990296	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050806050263993145	0.0
2	0.0	0.0	0.0	0.17999318179028057	0.0
3	0.0	0.0	0.0	0.24524779281708217	0.0
4	0.0	0.0	0.0	0.36329158989885063	0.0
5	0.0	0.0	0.0	0.5946574321791168	0.0
6	0.0	0.0	0.0	0.8335780774726163	0.0
7	0.0	0.0	0.0	0.9889237144322235	0.0
8	0.0	0.0	0.0	1.4567548910266883	0.0
9	0.0	0.0	0.0	1.7006805933164546	0.0
10	0.0	0.0	0.0	2.155857474863754	0.0
11	0.0	0.0	0.0	2.5198101100262624	0.0
12	0.0	0.0	0.0	2.850710482005876	0.0
13	0.0	0.0	0.0	2.975553601799554	0.0
14	0.0	0.0	0.0	3.022015640330938	0.0
15	0.0	0.0	0.0	3.090481042638364	0.0
16	0.0	0.0	0.0	3.2445990241083207	0.0
17	0.0	0.0	0.0	3.424969946049263	0.0
18	0.0	0.0	0.0	3.6980760749776427	0.0
19	0.0	0.0	0.0	3.8130035158164524	0.0
20	0.0	0.0	0.0	3.9310473128982206	0.0
21	0.0	0.0	0.0	4.077043881128952	0.0
22	0.0	0.0	0.0	4.221435053719371	0.0
23	0.0	0.0	0.0	4.395667698212061	0.0
24	0.0	0.0	0.0	4.52504369981368	0.0
25	0.0	0.0	0.0	4.620234217780418	0.0
26	0.0	0.0	0.0	4.722318493496731	0.0
27	0.0	0.0	0.0	4.823363983798725	0.0
28	0.0	0.0	0.0	4.926014869741031	0.0
29	0.0	0.0	0.0	5.036126123658905	0.0
30	0.0	0.0	0.0	5.197137862878437	0.0
31	0.0	0.0	0.0	5.3231142031241	0.0
32	0.0	0.0	0.0	5.428125965008041	0.0
33	0.0	0.0	0.0	5.540881399980546	0.0
34	0.0	0.0	0.0	5.664213559171578	0.0
35	0.0	0.0	0.0	5.828719394784731	0.0
36	0.0	0.0	0.0	5.959228616838334	0.0
37	0.0	0.0	0.0	6.082183035878704	0.0
38	0.0	0.0	0.0	6.209481466651683	0.0
39	0.0	0.0	0.0	6.338196422989643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.033033E-4	45.000004	2
ACTATCG	35	1.2118107E-7	45.000004	18
CGAACGC	20	7.033033E-4	45.000004	31
ACGTTCA	30	2.1653395E-6	45.000004	12
CGGCCCA	20	7.033033E-4	45.000004	36
CGCGATC	20	7.033033E-4	45.000004	40
AGTGCGC	20	7.033033E-4	45.000004	32
CTACGTC	20	7.033033E-4	45.000004	21
TGTTACG	35	1.2118107E-7	45.000004	1
TGCTCGA	20	7.033033E-4	45.000004	30
ACTCACG	30	2.1653395E-6	45.000004	1
GTGACCG	20	7.033033E-4	45.000004	2
CCACGTG	20	7.033033E-4	45.000004	37
TCGAATC	20	7.033033E-4	45.000004	33
CTCCGTA	25	3.8905975E-5	45.0	44
ATCGTAT	50	2.1827873E-11	45.0	13
TAACATG	55	1.8189894E-12	45.0	1
TAACACG	25	3.8905975E-5	45.0	1
ACTACGG	50	2.1827873E-11	45.0	2
TCCGTAG	25	3.8905975E-5	45.0	45
>>END_MODULE