FastQCFastQC Report
Thu 26 May 2016
SRR1546305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546305_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3959557
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453041.1441684006569421No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT99150.2504068005587494No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC62660.15825002645497968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT336850.043.931281
TAATGCG1800.040.0000041
ATACTAT51100.039.05577545
CGAATAT13300.038.5714314
GCTACGA13300.038.5714310
AGGGCGA40500.038.56
GTTTTTT392300.038.467372
TAAGGGA44500.038.325844
CGTAAGG4750.037.8947372
GCGATGT8150.037.8220869
AAGGGAT52600.037.7709125
GCGCGAC19950.037.556399
CGACTTA301.14042596E-437.50000443
AGCTACG13650.037.2527479
TACGAAT13850.037.0397112
TGATTCG10050.036.940315
TGGGCGA22950.036.8627476
GGCGATT25050.036.8263478
GGGCGAT79850.036.7720767
ATATCAC16050.036.7289730
Produced by FastQC (version 0.11.5)