##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546305_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3959557
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.095036641725326	31.0	31.0	33.0	30.0	34.0
2	31.435637117990726	31.0	31.0	34.0	30.0	34.0
3	31.38824267462244	31.0	31.0	34.0	30.0	34.0
4	35.31519182575222	37.0	35.0	37.0	33.0	37.0
5	35.2527770152065	37.0	35.0	37.0	33.0	37.0
6	35.29996183916534	37.0	35.0	37.0	32.0	37.0
7	35.777561732284695	37.0	35.0	37.0	35.0	37.0
8	35.69305253087656	37.0	35.0	37.0	35.0	37.0
9	37.443779948110354	39.0	37.0	39.0	35.0	39.0
10	36.89414699674737	39.0	37.0	39.0	32.0	39.0
11	36.62221480837376	39.0	35.0	39.0	32.0	39.0
12	36.19746350412432	38.0	35.0	39.0	32.0	39.0
13	36.00364459963577	38.0	35.0	39.0	31.0	39.0
14	36.96128354762919	39.0	35.0	41.0	31.0	41.0
15	37.1537558368272	39.0	35.0	41.0	32.0	41.0
16	37.17637705430178	39.0	35.0	41.0	32.0	41.0
17	37.10828660883023	39.0	35.0	41.0	32.0	41.0
18	36.96296883717042	39.0	35.0	40.0	32.0	41.0
19	36.78679458333344	38.0	35.0	40.0	31.0	41.0
20	36.567850393364715	38.0	35.0	40.0	31.0	41.0
21	36.4368433135323	38.0	35.0	40.0	31.0	41.0
22	36.35849464978027	38.0	35.0	40.0	31.0	41.0
23	36.274131929405236	38.0	35.0	40.0	30.0	41.0
24	36.22060902267602	38.0	35.0	40.0	30.0	41.0
25	36.146498206743836	38.0	35.0	40.0	30.0	41.0
26	36.040969987299086	38.0	35.0	40.0	30.0	41.0
27	35.93367490353087	38.0	35.0	40.0	30.0	41.0
28	35.890074066366516	38.0	35.0	40.0	30.0	41.0
29	35.75945819191389	37.0	34.0	40.0	30.0	41.0
30	35.54825249390273	37.0	34.0	40.0	29.0	41.0
31	35.29480343381848	37.0	34.0	40.0	27.0	41.0
32	35.02932247218565	37.0	34.0	40.0	25.0	41.0
33	34.701201169726815	37.0	33.0	40.0	24.0	41.0
34	34.33790345738172	37.0	33.0	40.0	23.0	41.0
35	34.04762527727218	37.0	33.0	40.0	21.0	41.0
36	33.80452080876724	37.0	33.0	40.0	20.0	41.0
37	33.6215654933115	37.0	33.0	40.0	18.0	41.0
38	33.49705282686927	36.0	32.0	40.0	18.0	41.0
39	33.417941198977566	36.0	32.0	40.0	18.0	41.0
40	33.20615816365316	36.0	32.0	40.0	18.0	41.0
41	33.08250518934315	36.0	32.0	40.0	16.0	41.0
42	32.940894903141945	36.0	31.0	40.0	15.0	41.0
43	32.920608290270856	36.0	31.0	40.0	15.0	41.0
44	32.67901863769104	35.0	31.0	40.0	15.0	41.0
45	32.66440942762031	35.0	31.0	40.0	15.0	41.0
46	32.5855614655882	35.0	31.0	40.0	15.0	41.0
47	32.511278660718865	35.0	31.0	40.0	15.0	41.0
48	32.40690360057956	35.0	31.0	39.0	15.0	41.0
49	32.41005774130793	35.0	31.0	39.0	15.0	41.0
50	32.27749063847294	35.0	31.0	39.0	14.0	41.0
51	32.071631245616615	35.0	30.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	11.0
12	15.0
13	31.0
14	48.0
15	179.0
16	561.0
17	1447.0
18	3310.0
19	6463.0
20	10947.0
21	17037.0
22	25505.0
23	37367.0
24	53541.0
25	78271.0
26	101533.0
27	108209.0
28	106573.0
29	109432.0
30	121744.0
31	140927.0
32	168399.0
33	210796.0
34	317060.0
35	411987.0
36	305924.0
37	382090.0
38	527593.0
39	712444.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.624930263663334	23.60185748052118	28.383149933186967	18.390062322628516
2	34.774521493187244	24.673846089347872	28.0327066891574	12.518925728307483
3	26.487180257791465	23.932929870690078	36.4220795407163	13.157810330802159
4	24.009226284657604	26.528826330824383	34.853141399404024	14.60880598511399
5	21.345670740438894	29.969362734265477	34.411248531085675	14.273717994209958
6	19.242834488807713	38.66629524464479	31.786459949938845	10.304410316608651
7	82.81911335030662	3.635305666770298	11.733863156913765	1.8117178260093236
8	83.6001603209652	3.023823119606562	11.354022684860958	2.021993874567281
9	78.69478833111886	5.1081724546458105	13.18788945328985	3.0091497609454794
10	39.36601998657931	28.505385829778433	19.686596253065684	12.441997930576578
11	32.26866035771173	22.819093146026184	29.015846974800464	15.896399521461618
12	30.680502894642004	21.064553433629065	31.81724622224153	16.437697449487406
13	22.580278551363193	29.132021587263424	31.089488041212693	17.19821182016069
14	18.115864981865396	32.15210691499074	32.079548292902466	17.6524798102414
15	17.18821070134866	23.31505771984088	42.210681649487555	17.286049929322903
16	19.28965790870039	20.40735870199621	39.78311210067187	20.51987128863153
17	19.145399346442037	21.24586159512289	32.49926696345071	27.109472094984362
18	21.669343312900914	22.463775619343277	35.843100629691655	20.023780438064158
19	25.561218085760608	24.891547210963246	29.42877195605468	20.118462747221468
20	26.828455809576678	23.506695319703695	29.700317485011578	19.96453138570805
21	22.731734888524148	25.581472876890015	31.52256679219418	20.16422544239166
22	22.13328410223669	23.313820207664644	30.1804722093911	24.372423480707564
23	20.241279516875245	26.8516402213682	29.830988668681872	23.07609159307468
24	20.52292718604632	23.94639097252546	36.870437778771716	18.660244062656503
25	20.247997440117672	25.66913419859848	33.2960732728434	20.78679508844045
26	19.290213526412174	28.514932352280827	30.430727477846638	21.764126643460365
27	18.735353475148862	27.343513428396154	31.98130497931966	21.939828117135328
28	17.829343030040988	25.78088912471774	35.71295980838261	20.676808036858667
29	18.68587824344996	23.43974338543428	35.58787005718064	22.28650831393512
30	20.97628598350775	26.513319545595632	32.93865955206605	19.571734918830565
31	23.93348548840186	26.182322921478335	29.95923533870077	19.924956251419033
32	24.700692526967032	25.462545431218697	29.209883832964138	20.62687820885013
33	24.213239006282773	25.90898426263342	28.58877899724641	21.288997733837398
34	19.718796824998353	25.830642165272526	31.48849732432189	22.96206368540723
35	20.871981385796442	25.649889621490484	31.290116545866116	22.188012446846958
36	24.447457127148315	26.729909431787448	27.93160952096409	20.891023920100153
37	21.380169549270285	29.043956179946395	29.42271067192618	20.153163598857144
38	21.44224720088636	28.257832883830186	28.927377481874867	21.372542433408587
39	21.284527536792627	28.782740089358484	27.926659472259146	22.00607290158975
40	23.214591935410954	25.463050538229403	28.7455389580198	22.576818568339842
41	20.183217465994304	25.206052091180908	28.631233241496464	25.979497201328332
42	21.536323381630822	26.74296644801426	27.119523724497462	24.601186445857454
43	21.587970573475772	26.275540420304594	28.64002210348279	23.496466902736845
44	21.72341501839726	27.17738373257412	29.06158441462012	22.037616834408496
45	19.529735270890153	30.57599625412641	26.96768855707848	22.926579917904956
46	21.503516681285305	28.614185879885046	28.133829112701243	21.748468326128403
47	21.191840400327614	27.803792191904297	28.676061488696842	22.32830591907125
48	20.975452556940084	25.848219889245183	30.172643050725117	23.003684503089612
49	20.98441820638016	25.271564470469805	30.725533184646665	23.01848413850337
50	20.139955050527117	28.202801475013494	29.173314085388846	22.483929389070546
51	19.482507765388906	28.555896530849285	28.69063382595578	23.270961877806027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1683.0
1	2641.5
2	3600.0
3	38986.0
4	74372.0
5	53165.5
6	31959.0
7	31979.0
8	31999.0
9	32963.5
10	33928.0
11	34063.0
12	34198.0
13	33550.0
14	32902.0
15	31894.5
16	30887.0
17	29561.0
18	28235.0
19	26828.5
20	25422.0
21	24417.0
22	23412.0
23	24094.5
24	24777.0
25	26786.0
26	30699.0
27	32603.0
28	39038.5
29	45474.0
30	52672.0
31	59870.0
32	72129.5
33	84389.0
34	101965.5
35	119542.0
36	123820.5
37	128099.0
38	143950.0
39	159801.0
40	183609.5
41	207418.0
42	238247.0
43	269076.0
44	280687.0
45	292298.0
46	300495.5
47	308693.0
48	305457.0
49	302221.0
50	294821.5
51	287422.0
52	261049.5
53	234677.0
54	220185.0
55	205693.0
56	192637.5
57	179582.0
58	172169.5
59	164757.0
60	148456.0
61	132155.0
62	116026.5
63	99898.0
64	90246.5
65	80595.0
66	66455.0
67	52315.0
68	44656.5
69	36998.0
70	31868.0
71	26738.0
72	22464.0
73	18190.0
74	15025.0
75	9424.0
76	6988.0
77	4763.5
78	2539.0
79	2176.0
80	1813.0
81	1392.0
82	971.0
83	665.5
84	360.0
85	283.0
86	206.0
87	144.5
88	83.0
89	58.5
90	34.0
91	25.5
92	17.0
93	13.0
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3959557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.912279128948875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11703656860541	18.356639048440915
2	7.21645662394573	3.306909369795932
3	2.6424830724237176	1.8163592924668392
4	1.3723024892255233	1.257703107299462
5	0.8774493127082381	1.0052181787137748
6	0.6778925828858345	0.931923844711461
7	0.502144847646917	0.8053698038714767
8	0.40825771648499043	0.7483291805321097
9	0.3518863057961283	0.7256265534049997
>10	3.782090098056437	19.8647154935362
>50	1.1874608039787207	19.65675637163659
>100	0.8577053140995778	28.718870357605443
>500	0.004817596035136501	0.7390980394607263
>1k	0.001680556756442858	0.4881456070569768
>5k	2.2407423419238106E-4	0.4153704284701799
>10k+	1.1203711709619053E-4	1.162965322996912
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45304	1.1441684006569421	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	9915	0.2504068005587494	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6266	0.15825002645497968	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034119978573360604	0.0
2	0.0	0.0	0.0	0.1604977526526326	0.0
3	0.0	0.0	0.0	0.29172455403470643	0.0
4	0.0	0.0	0.0	0.4318664941557856	0.0
5	0.0	0.0	0.0	0.7465734171777297	0.0
6	0.0	0.0	0.0	1.2099080781006561	0.0
7	0.0	0.0	0.0	1.5057997649737078	0.0
8	0.0	0.0	0.0	2.1302383069621174	0.0
9	0.0	0.0	0.0	2.502552684555368	0.0
10	0.0	0.0	0.0	2.9955371270068847	0.0
11	0.0	0.0	0.0	3.382196543704258	0.0
12	0.0	0.0	0.0	3.7012726423688305	0.0
13	0.0	0.0	0.0	3.860987479154865	0.0
14	0.0	0.0	0.0	3.9350866776258053	0.0
15	0.0	0.0	0.0	4.016484672401483	0.0
16	0.0	0.0	0.0	4.18165466490317	0.0
17	0.0	0.0	0.0	4.373570073621872	0.0
18	0.0	0.0	0.0	4.64458524021753	0.0
19	0.0	0.0	0.0	4.769952800275385	0.0
20	0.0	0.0	0.0	4.915878215669076	0.0
21	0.0	0.0	0.0	5.076325457620638	0.0
22	0.0	0.0	0.0	5.252405761553629	0.0
23	0.0	0.0	0.0	5.452125073587777	0.0
24	0.0	0.0	0.0	5.612092463879167	0.0
25	0.0	0.0	0.0	5.73708119367899	0.0
26	0.0	0.0	0.0	5.865984502811805	0.0
27	0.0	0.0	0.0	6.017844925581321	0.0
28	0.0	0.0	0.0	6.159805250940951	0.0
29	0.0	0.0	0.0	6.318863448613064	0.0
30	0.0	0.0	0.0	6.530427520048328	0.0
31	0.0	0.0	0.0	6.6864803310067265	0.0
32	0.0	0.0	0.0	6.841927013552274	0.0
33	0.0	0.0	0.0	7.002172212699552	0.0
34	0.0	0.0	0.0	7.166609800035711	0.0
35	0.0	0.0	0.0	7.364005619820601	0.0
36	0.0	0.0	0.0	7.533898362872412	0.0
37	0.0	0.0	0.0	7.7103069863623634	0.0
38	0.0	0.0	0.0	7.905783399506561	0.0
39	0.0	0.0	0.0	8.16293337865827	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33685	0.0	43.93128	1
TAATGCG	180	0.0	40.000004	1
ATACTAT	5110	0.0	39.055775	45
CGAATAT	1330	0.0	38.57143	14
GCTACGA	1330	0.0	38.57143	10
AGGGCGA	4050	0.0	38.5	6
GTTTTTT	39230	0.0	38.46737	2
TAAGGGA	4450	0.0	38.32584	4
CGTAAGG	475	0.0	37.894737	2
GCGATGT	815	0.0	37.822086	9
AAGGGAT	5260	0.0	37.770912	5
GCGCGAC	1995	0.0	37.55639	9
CGACTTA	30	1.14042596E-4	37.500004	43
AGCTACG	1365	0.0	37.252747	9
TACGAAT	1385	0.0	37.03971	12
TGATTCG	1005	0.0	36.9403	15
TGGGCGA	2295	0.0	36.862747	6
GGCGATT	2505	0.0	36.826347	8
GGGCGAT	7985	0.0	36.772076	7
ATATCAC	1605	0.0	36.72897	30
>>END_MODULE