FastQCFastQC Report
Thu 26 May 2016
SRR1546304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546304_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4309467
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246460.5719036716141462No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA158310.3673540138490444No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC108630.2520729361658878No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT44650.10360910061499484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA453.8562575E-1045.042
CGTTTTT185800.043.316741
TCGATAG1700.041.029411
TGCATTA29550.040.96446615
ACTTGAC33600.040.446436
TAATGCG1350.040.01
ATTAGCG3900.039.8076931
AACTTGA34200.039.7368435
CATTAGA30350.039.6622717
TCGTAAG2100.039.6428571
GCATTAG30500.039.61475416
AGTACGG3750.039.6000022
CTCAACT35200.039.566762
TCAACTT35500.039.1690143
CGGGTAT3050.039.098366
GCTCAAC35750.039.0839161
TAGGGAC27800.038.8489235
CTTGACC35100.038.717957
CGGTCTA5550.038.5135131
TACGGGA9200.038.1521724
Produced by FastQC (version 0.11.5)