##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546304_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4309467
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07800198957319	31.0	31.0	33.0	30.0	34.0
2	31.408923655756038	31.0	31.0	34.0	30.0	34.0
3	31.4872723239324	31.0	31.0	34.0	30.0	34.0
4	35.35674713369426	37.0	35.0	37.0	33.0	37.0
5	35.26932727411534	37.0	35.0	37.0	33.0	37.0
6	35.303073210677795	37.0	35.0	37.0	32.0	37.0
7	35.73882408195723	37.0	35.0	37.0	35.0	37.0
8	35.63380088535311	37.0	35.0	37.0	35.0	37.0
9	37.36957076130297	39.0	37.0	39.0	35.0	39.0
10	36.86005833203967	39.0	37.0	39.0	32.0	39.0
11	36.57229420714905	39.0	35.0	39.0	32.0	39.0
12	35.96454805199808	37.0	35.0	39.0	31.0	39.0
13	35.67196569784616	37.0	35.0	39.0	30.0	39.0
14	36.56799808421784	38.0	35.0	41.0	31.0	41.0
15	36.83308678312191	38.0	35.0	41.0	31.0	41.0
16	36.910552743529536	38.0	35.0	41.0	32.0	41.0
17	36.83071247557993	38.0	35.0	41.0	31.0	41.0
18	36.73995624052812	38.0	35.0	40.0	31.0	41.0
19	36.630685650916924	38.0	35.0	40.0	31.0	41.0
20	36.443352971492764	38.0	35.0	40.0	31.0	41.0
21	36.26803198632221	38.0	35.0	40.0	30.0	41.0
22	36.18017054081166	38.0	35.0	40.0	30.0	41.0
23	36.078989582702455	38.0	34.0	40.0	30.0	41.0
24	36.05333118921667	38.0	34.0	40.0	30.0	41.0
25	35.993417283390265	38.0	34.0	40.0	30.0	41.0
26	35.88156029504345	37.0	34.0	40.0	30.0	41.0
27	35.74591057316369	37.0	34.0	40.0	30.0	41.0
28	35.67936406056712	37.0	34.0	40.0	29.0	41.0
29	35.582795737848784	37.0	34.0	40.0	29.0	41.0
30	35.400865118586594	37.0	34.0	40.0	28.0	41.0
31	35.20148709805644	37.0	34.0	40.0	27.0	41.0
32	35.012357676715006	37.0	34.0	40.0	26.0	41.0
33	34.79718025454192	37.0	33.0	40.0	25.0	41.0
34	34.55027965175276	37.0	33.0	40.0	24.0	41.0
35	34.32814754121566	36.0	33.0	40.0	23.0	41.0
36	34.06988752901461	36.0	33.0	40.0	23.0	41.0
37	33.887815360925146	36.0	33.0	40.0	23.0	41.0
38	33.7695494593647	36.0	33.0	40.0	22.0	41.0
39	33.68673945060955	36.0	33.0	40.0	22.0	41.0
40	33.470974020685155	35.0	32.0	40.0	21.0	41.0
41	33.35139821235433	35.0	32.0	40.0	20.0	41.0
42	33.21630818846043	35.0	32.0	40.0	20.0	41.0
43	33.16653312346979	35.0	32.0	40.0	20.0	41.0
44	32.994456391010765	35.0	32.0	39.0	19.0	41.0
45	32.926722260548694	35.0	31.0	39.0	20.0	41.0
46	32.85273399239396	35.0	31.0	39.0	20.0	41.0
47	32.76970817968904	35.0	31.0	39.0	20.0	41.0
48	32.67671802568624	35.0	31.0	39.0	19.0	41.0
49	32.68113968618393	35.0	31.0	39.0	19.0	41.0
50	32.52301293872305	35.0	31.0	39.0	18.0	40.0
51	32.30029235634012	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	8.0
12	15.0
13	32.0
14	94.0
15	230.0
16	673.0
17	1667.0
18	3537.0
19	6662.0
20	11534.0
21	17966.0
22	26665.0
23	37523.0
24	52060.0
25	70393.0
26	89348.0
27	101303.0
28	108761.0
29	119699.0
30	138559.0
31	163045.0
32	197083.0
33	249864.0
34	392375.0
35	511923.0
36	343099.0
37	425197.0
38	562964.0
39	677080.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.57717624940625	23.023752125262824	28.012443302153144	14.386628323177785
2	32.14986911374423	24.486392400730765	29.394725612239288	13.969012873285722
3	28.35060577096889	24.878552266440373	33.03965432384098	13.731187638749756
4	25.63924958701389	27.04301947317383	30.965871185462145	16.35185975435013
5	23.077053380383237	31.87816497956708	30.318877021218633	14.725904618831054
6	20.438003121963806	40.862570707700044	27.806362132486456	10.893064037849692
7	84.35931868140538	4.4979344313345475	8.16393303394596	2.9788138533141106
8	84.61658947614636	3.926680492042287	8.648470912992256	2.808259118819102
9	79.93505925442751	5.508105758786411	10.800802048141916	3.756032938644153
10	47.913349841175254	23.94054763616939	15.850150378225427	12.295952144429927
11	38.93337621566658	23.878939089219152	22.725385761162574	14.462298933951692
12	34.000747656264686	21.240260106412233	27.031904409524426	17.727087827798655
13	24.102516622125194	31.72712541945442	26.779251355213994	17.39110660320638
14	20.057167162435633	33.695767945316675	28.58351160363915	17.663553288608544
15	17.89258393207327	21.53864967523826	42.26209412904194	18.306672263646526
16	20.969739413249943	18.428543483451666	39.56463757583014	21.037079527468247
17	20.31791866604385	18.934684962200663	29.480490278728205	31.26690609302728
18	23.72590392268928	22.31728424884098	32.505481536347766	21.451330292121973
19	29.20546786876428	24.018793971505062	26.38314668612151	20.39259147360915
20	30.803090034103985	23.507965138148172	24.95886382237061	20.730081005377233
21	25.24836598122227	27.14036329782778	27.41062873900647	20.200641981943473
22	25.863221600258225	22.984907414304367	25.736732640022534	25.415138345414874
23	23.211269514304206	27.976151111030667	25.011631368798042	23.800948005867085
24	22.276211884207488	25.347218113052033	33.16897426062202	19.207595742118457
25	21.209861915638292	24.830124003734106	31.031447740521045	22.92856634010656
26	20.270836277432917	28.891623952567684	27.949976180348983	22.887563589650416
27	20.368644196602503	27.65956903719184	28.794883450784052	23.176903315421605
28	20.12668852087741	24.605989557409302	34.069387235126754	21.19793468658653
29	19.697888393158596	24.227218818475695	33.57287571757714	22.50201707078857
30	21.431026157063044	26.667033301334016	30.138437073540647	21.763503468062293
31	28.005969183660067	24.402785773739534	25.18940277301114	22.401842269589256
32	28.497375661537728	24.792509143242075	24.872008533769954	21.838106661450244
33	27.124676903199397	25.255977131278645	24.537674844708175	23.08167112081378
34	21.616710372767674	26.557808657079867	28.06683517938529	23.75864579076716
35	23.61015875049049	25.681807054097412	28.19033072999515	22.517703465416954
36	27.753548176607456	25.859369615778473	25.064514938854387	21.322567268759688
37	23.09812327139296	28.865240179353968	25.858418222021424	22.17821832723165
38	23.97632932332467	29.22075978305438	23.535764399634573	23.26714649398638
39	22.36327601534018	27.7590476966177	25.24282005176046	24.634856236281657
40	24.366006283375647	23.713303756589852	26.74036023480398	25.18032972523052
41	21.562991432583196	22.939565380127057	26.71358198125197	28.783861206037777
42	22.884523770573022	24.290150034795484	24.42657061766571	28.398755576965783
43	23.194492497564084	24.768051362268235	26.182565036465068	25.854891103702617
44	22.479415667877255	26.4841104479974	27.384430603599007	23.65204328052634
45	20.53298006458803	29.993013057067152	25.032005118034316	24.442001760310497
46	23.106082492336057	27.30418866184612	27.109176146377266	22.480552699440555
47	23.618373223417187	25.4427751738208	27.028539724286087	23.910311878475923
48	23.566371432940546	23.56059345622092	28.7100469733264	24.162988137512134
49	22.716544760639774	23.00994531342275	29.10742790233688	25.1660820236006
50	21.381368043890348	28.023001452383788	27.056338985772488	23.53929151795338
51	21.62330051488966	28.72865716340327	25.15854048772157	24.489501833985504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1809.0
1	3132.0
2	4455.0
3	23277.0
4	42099.0
5	30253.5
6	18408.0
7	19203.0
8	19998.0
9	21067.5
10	22137.0
11	22252.0
12	22367.0
13	22095.0
14	21823.0
15	21178.5
16	20534.0
17	19438.0
18	18342.0
19	17466.5
20	16591.0
21	17125.5
22	17660.0
23	18295.5
24	18931.0
25	22806.5
26	30238.0
27	33794.0
28	35154.5
29	36515.0
30	49321.5
31	62128.0
32	64855.5
33	67583.0
34	78000.5
35	88418.0
36	97182.0
37	105946.0
38	124486.0
39	143026.0
40	180539.5
41	218053.0
42	253027.0
43	288001.0
44	294906.5
45	301812.0
46	298392.5
47	294973.0
48	302636.0
49	310299.0
50	316277.0
51	322255.0
52	302166.5
53	282078.0
54	263770.5
55	245463.0
56	232417.5
57	219372.0
58	214341.5
59	209311.0
60	200815.5
61	192320.0
62	187969.5
63	183619.0
64	169042.5
65	154466.0
66	128700.5
67	102935.0
68	85267.5
69	67600.0
70	54407.0
71	41214.0
72	34021.5
73	26829.0
74	22035.0
75	13746.5
76	10252.0
77	8477.0
78	6702.0
79	4838.0
80	2974.0
81	2172.5
82	1371.0
83	979.0
84	587.0
85	417.5
86	248.0
87	193.5
88	139.0
89	101.0
90	63.0
91	47.0
92	31.0
93	18.5
94	6.0
95	5.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4309467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.26946507354427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6854939935327	15.740357735235888
2	7.48199808092322	2.8834820140135053
3	2.438758466421455	1.4098071327455588
4	1.1832838769753664	0.9120498935785951
5	0.7307098288073346	0.7040193762549225
6	0.46861823645600625	0.5418013644128954
7	0.3736558619244634	0.504010400661497
8	0.2726771898228237	0.42034748685144824
9	0.23329639260135673	0.4045947020114144
>10	2.5490375330474677	11.52538127050335
>50	1.111090295179306	15.745815982308143
>100	1.4604317422751725	46.240287909767716
>500	0.008150551513711225	0.9890591344121029
>1k	0.0024330004518540965	0.7755088129252623
>5k	0.0	0.0
>10k+	3.649500677781145E-4	1.2034767843177152
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24646	0.5719036716141462	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	15831	0.3673540138490444	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10863	0.2520729361658878	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	4465	0.10360910061499484	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3204725781633784E-5	0.0	0.0	0.0445762782265185	0.0
2	2.3204725781633784E-5	0.0	0.0	0.17039230141453687	0.0
3	2.3204725781633784E-5	0.0	0.0	0.2441601246743507	0.0
4	2.3204725781633784E-5	0.0	0.0	0.3788635578367348	0.0
5	2.3204725781633784E-5	0.0	0.0	0.6572274483132137	0.0
6	2.3204725781633784E-5	0.0	0.0	1.0167614695738476	0.0
7	2.3204725781633784E-5	0.0	0.0	1.246233002828424	0.0
8	2.3204725781633784E-5	0.0	0.0	1.9242750901677632	0.0
9	2.3204725781633784E-5	0.0	0.0	2.2559402357646547	0.0
10	2.3204725781633784E-5	0.0	0.0	2.754029674667424	0.0
11	2.3204725781633784E-5	0.0	0.0	3.1053492230013595	0.0
12	4.640945156326757E-5	0.0	0.0	3.4111410993517297	0.0
13	4.640945156326757E-5	0.0	0.0	3.567889022006666	0.0
14	4.640945156326757E-5	0.0	0.0	3.6192874896129847	0.0
15	4.640945156326757E-5	0.0	0.0	3.7192070388286997	0.0
16	4.640945156326757E-5	0.0	0.0	3.870246598941354	0.0
17	4.640945156326757E-5	0.0	0.0	4.045651121124724	0.0
18	4.640945156326757E-5	0.0	0.0	4.303269986752422	0.0
19	4.640945156326757E-5	0.0	0.0	4.417924536839474	0.0
20	4.640945156326757E-5	0.0	0.0	4.551142867551834	0.0
21	4.640945156326757E-5	0.0	0.0	4.7014166717136945	0.0
22	4.640945156326757E-5	0.0	0.0	4.859928153528035	0.0
23	4.640945156326757E-5	0.0	0.0	5.051297527049169	0.0
24	4.640945156326757E-5	0.0	0.0	5.198206645972692	0.0
25	4.640945156326757E-5	0.0	0.0	5.355673915126859	0.0
26	4.640945156326757E-5	0.0	0.0	5.472834575598328	2.3204725781633784E-5
27	4.640945156326757E-5	0.0	0.0	5.592756598437812	2.3204725781633784E-5
28	4.640945156326757E-5	0.0	0.0	5.718340574368013	2.3204725781633784E-5
29	4.640945156326757E-5	0.0	0.0	5.857244062896873	2.3204725781633784E-5
30	4.640945156326757E-5	0.0	0.0	6.0354795616256025	2.3204725781633784E-5
31	4.640945156326757E-5	0.0	0.0	6.184082625531185	2.3204725781633784E-5
32	4.640945156326757E-5	0.0	0.0	6.351087036981603	2.3204725781633784E-5
33	4.640945156326757E-5	0.0	0.0	6.495954140036344	2.3204725781633784E-5
34	4.640945156326757E-5	0.0	0.0	6.677461505100283	2.3204725781633784E-5
35	4.640945156326757E-5	0.0	0.0	6.863168925530698	2.3204725781633784E-5
36	4.640945156326757E-5	0.0	0.0	7.015554359738687	2.3204725781633784E-5
37	4.640945156326757E-5	0.0	0.0	7.179194085950768	2.3204725781633784E-5
38	4.640945156326757E-5	0.0	0.0	7.361188750256122	2.3204725781633784E-5
39	4.640945156326757E-5	0.0	0.0	7.618923639512729	2.3204725781633784E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	45	3.8562575E-10	45.0	42
CGTTTTT	18580	0.0	43.31674	1
TCGATAG	170	0.0	41.02941	1
TGCATTA	2955	0.0	40.964466	15
ACTTGAC	3360	0.0	40.44643	6
TAATGCG	135	0.0	40.0	1
ATTAGCG	390	0.0	39.807693	1
AACTTGA	3420	0.0	39.736843	5
CATTAGA	3035	0.0	39.66227	17
TCGTAAG	210	0.0	39.642857	1
GCATTAG	3050	0.0	39.614754	16
AGTACGG	375	0.0	39.600002	2
CTCAACT	3520	0.0	39.56676	2
TCAACTT	3550	0.0	39.169014	3
CGGGTAT	305	0.0	39.09836	6
GCTCAAC	3575	0.0	39.083916	1
TAGGGAC	2780	0.0	38.848923	5
CTTGACC	3510	0.0	38.71795	7
CGGTCTA	555	0.0	38.51351	31
TACGGGA	920	0.0	38.152172	4
>>END_MODULE