FastQCFastQC Report
Thu 26 May 2016
SRR1546303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546303_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1818331
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125290.6890384643939965No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25310.13919357916682937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG502.1827873E-1145.01
TGTTACG850.045.01
CGTTTTT90300.043.480071
CGACGGT1950.040.38461728
TTACGGG4650.039.677423
ATTAGCG1250.039.6000021
GTTACGG1650.038.1818162
CGGTCTA2100.037.531
TACGGGA3850.037.40264
GCGCGAC7400.037.0945939
GTTTTTT112550.036.923592
CGAACTA250.002107430536.024
TATGCGA250.002107430536.01
TAGGGAC12050.035.8506245
GTAGGGT8800.035.7954564
CGTCATA6700.035.5970138
TAGGGTA8750.035.4857145
TAGACGG2600.035.480772
CGTATGG2350.035.4255332
TTGTAGG10900.035.298172
Produced by FastQC (version 0.11.5)