##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546303_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1818331 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.133736376930273 31.0 31.0 33.0 30.0 34.0 2 31.46177566130699 31.0 31.0 34.0 30.0 34.0 3 31.51390533406734 31.0 31.0 34.0 30.0 34.0 4 35.39784230703871 37.0 35.0 37.0 33.0 37.0 5 35.32378813318367 37.0 35.0 37.0 33.0 37.0 6 35.35818946055476 37.0 35.0 37.0 33.0 37.0 7 35.76483379538709 37.0 35.0 37.0 35.0 37.0 8 35.66434549045251 37.0 35.0 37.0 35.0 37.0 9 37.39383808558508 39.0 37.0 39.0 35.0 39.0 10 36.89993021072621 39.0 37.0 39.0 32.0 39.0 11 36.6265729396903 39.0 35.0 39.0 32.0 39.0 12 36.104748255405646 38.0 35.0 39.0 32.0 39.0 13 35.887959342935915 38.0 35.0 39.0 31.0 39.0 14 36.812403792268846 39.0 35.0 41.0 31.0 41.0 15 37.02307665656033 39.0 35.0 41.0 31.0 41.0 16 37.06216194961203 39.0 35.0 41.0 32.0 41.0 17 36.99324105457147 38.0 35.0 41.0 32.0 41.0 18 36.89028070246836 38.0 35.0 40.0 31.0 41.0 19 36.77666277481933 38.0 35.0 40.0 31.0 41.0 20 36.60656393142943 38.0 35.0 40.0 31.0 41.0 21 36.4749316818555 38.0 35.0 40.0 31.0 41.0 22 36.38186941761428 38.0 35.0 40.0 30.0 41.0 23 36.28655673801965 38.0 35.0 40.0 30.0 41.0 24 36.26771473400608 38.0 35.0 40.0 30.0 41.0 25 36.20447432288181 38.0 35.0 40.0 30.0 41.0 26 36.08552568261774 38.0 35.0 40.0 30.0 41.0 27 35.97141499539963 38.0 35.0 40.0 30.0 41.0 28 35.90930474154596 38.0 35.0 40.0 30.0 41.0 29 35.777735736782795 38.0 34.0 40.0 29.0 41.0 30 35.59779270110887 37.0 34.0 40.0 29.0 41.0 31 35.37628682566596 37.0 34.0 40.0 27.0 41.0 32 35.19016559691277 37.0 34.0 40.0 27.0 41.0 33 34.92997369565827 37.0 34.0 40.0 25.0 41.0 34 34.674448161528346 37.0 33.0 40.0 24.0 41.0 35 34.413041409952314 37.0 33.0 40.0 23.0 41.0 36 34.2232096356494 37.0 33.0 40.0 23.0 41.0 37 34.026571619798595 37.0 33.0 40.0 22.0 41.0 38 33.933370766928576 36.0 33.0 40.0 22.0 41.0 39 33.840664323492256 36.0 33.0 40.0 22.0 41.0 40 33.64985967901334 36.0 33.0 40.0 21.0 41.0 41 33.528002327409034 36.0 32.0 40.0 20.0 41.0 42 33.410169545588786 36.0 32.0 40.0 20.0 41.0 43 33.37553503735018 35.0 32.0 40.0 20.0 41.0 44 33.193804098373725 35.0 32.0 40.0 19.0 41.0 45 33.14179706555077 35.0 32.0 40.0 20.0 41.0 46 33.07437919718687 35.0 32.0 39.0 20.0 41.0 47 32.9736896087676 35.0 31.0 39.0 19.0 41.0 48 32.865435940981044 35.0 31.0 39.0 19.0 41.0 49 32.847254982728664 35.0 31.0 39.0 19.0 41.0 50 32.72067681846705 35.0 31.0 39.0 18.0 41.0 51 32.5112232041361 35.0 31.0 39.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 9.0 12 3.0 13 14.0 14 42.0 15 114.0 16 284.0 17 682.0 18 1478.0 19 2839.0 20 4756.0 21 7405.0 22 10806.0 23 15318.0 24 20970.0 25 29166.0 26 37537.0 27 42259.0 28 45356.0 29 48662.0 30 55598.0 31 65636.0 32 79561.0 33 100319.0 34 157541.0 35 197887.0 36 145255.0 37 182008.0 38 250206.0 39 316580.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.78860559491094 22.665675281343166 26.596037795098916 15.949681328646983 2 33.76629447553828 24.25185513528615 28.99593088387098 12.985919505304588 3 29.246105357055452 24.250920211996604 32.983873673165114 13.519100757782823 4 25.98355304947229 27.425204761949285 31.710783130244163 14.880459058334264 5 22.647361783965625 31.087684255506836 31.48849136928315 14.776462591244389 6 21.482172387755586 39.2985655526964 28.552722249139457 10.666539810408555 7 84.58058516298738 4.327154956935783 9.027839265788241 2.0644206142885975 8 85.1009524668501 3.6562100079688467 8.73713311822765 2.5057044069534093 9 80.42342125828576 5.020593060339399 10.917759197857816 3.6382264835170277 10 44.88066254163846 23.61880207728956 17.834376689392638 13.666158691679348 11 37.1939432369574 23.77476928018056 23.604558245995914 15.426729236866116 12 32.22570588083248 22.381513596809384 28.346599161538794 17.046181360819347 13 24.01680442119724 29.076499273234628 29.61237530460626 17.294321000961872 14 19.995479370917614 31.745155310006812 30.85318349629413 17.40618182278144 15 19.097898017467667 22.263438284888725 41.12139098986928 17.517272707774328 16 21.67993616123797 19.86838479902724 38.27179979882651 20.17987924090828 17 21.889248987120606 20.591685452208647 30.13912208503292 27.379943475637823 18 24.676035331301065 22.575922645546935 32.03602644403027 20.712015579121733 19 28.886984822895283 23.569691106844683 26.96533249446883 20.577991575791206 20 29.00572008066738 24.337153136585144 26.285533271995032 20.371593510752444 21 26.264634986699342 25.24925329876684 28.677287028599302 19.80882468593452 22 24.664871247314156 23.316931845742054 27.88557198881832 24.132624918125465 23 23.319296651709728 26.61402131955073 27.13686342035636 22.929818608383183 24 23.0564732163726 24.086153731086366 32.401581450242006 20.45579160229903 25 22.808388571717693 23.800452172899213 30.992267084485718 22.398892170897376 26 21.075535752291525 27.789164899020037 28.311732022387563 22.82356732630088 27 21.442355654718533 26.343168543021044 29.992779092475462 22.221696709784965 28 20.765086224675265 25.193047910418947 33.61329702897877 20.428568835927013 29 21.77793812017724 24.49581511836954 32.540885020384074 21.185361741069144 30 24.111341664416436 24.99643904217659 30.51738104888494 20.374838244522035 31 27.41536056966526 24.196694661203047 26.88118939840986 21.506755370721834 32 28.5843446545211 24.844596500857104 26.84060272854612 19.730456116075676 33 27.01405849650036 24.794385620659824 27.621483657265923 20.57007222557389 34 23.680287032448987 25.26371711201096 28.803006713299173 22.252989142240878 35 23.286464345600443 25.60424917135549 29.847426018695167 21.2618604643489 36 28.475453589033016 25.70417597236147 25.728923941790576 20.091446496814935 37 23.884815250908666 28.854702471662204 26.995030057783758 20.26545221964538 38 25.186558442879765 28.42304288933093 24.597776752417463 21.792621915371843 39 23.32001159304879 27.713931071955543 26.01226069401006 22.95379664098561 40 24.90492655077651 24.93297424946283 26.946139069289366 23.215960130471295 41 21.782062781748756 24.798675268694204 26.81970444325043 26.59955750630661 42 23.77707909066061 26.730501762330398 25.518291224205054 23.974127922803934 43 23.097389859162057 25.862837954145864 27.135983492554438 23.903788694137646 44 23.04299932190564 27.176735148881033 27.775691004553078 22.00457452466025 45 21.43784602473367 28.55536203254523 26.33827394462284 23.668517998098256 46 23.912587972156885 27.416240497467182 27.46018189207576 21.210989638300177 47 22.894016545942407 26.373691038650275 28.048468623149468 22.683823792257847 48 23.542358349497423 25.277795956841743 28.426287623100528 22.753558070560313 49 22.937078012749055 24.615925263332144 29.474831590068035 22.972165133850766 50 22.04939584707075 27.5344807958507 27.58271183849365 22.8334115185849 51 21.522539075668842 28.847718044734428 26.499080750424426 23.130662129172304 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 749.0 1 1563.0 2 2377.0 3 12687.5 4 22998.0 5 16524.5 6 10051.0 7 10041.0 8 10031.0 9 10232.0 10 10433.0 11 10316.5 12 10200.0 13 10142.5 14 10085.0 15 9486.0 16 8887.0 17 8620.0 18 8353.0 19 8361.5 20 8370.0 21 8290.0 22 8210.0 23 8880.0 24 9550.0 25 10182.5 26 12261.5 27 13708.0 28 14857.5 29 16007.0 30 20014.0 31 24021.0 32 27254.0 33 30487.0 34 34986.5 35 39486.0 36 43579.0 37 47672.0 38 56155.5 39 64639.0 40 76916.0 41 89193.0 42 103000.0 43 116807.0 44 123127.5 45 129448.0 46 130530.5 47 131613.0 48 132057.0 49 132501.0 50 132865.5 51 133230.0 52 124988.5 53 116747.0 54 109882.5 55 103018.0 56 99821.0 57 96624.0 58 93769.0 59 90914.0 60 84181.0 61 77448.0 62 72089.5 63 66731.0 64 59401.5 65 52072.0 66 45942.0 67 39812.0 68 33409.5 69 27007.0 70 22385.0 71 17763.0 72 15847.5 73 13932.0 74 10734.0 75 5879.0 76 4222.0 77 3209.5 78 2197.0 79 1723.5 80 1250.0 81 933.5 82 617.0 83 437.0 84 257.0 85 196.5 86 136.0 87 96.5 88 57.0 89 41.5 90 26.0 91 23.0 92 20.0 93 19.0 94 18.0 95 12.0 96 6.0 97 3.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1818331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.082766062886105 #Duplication Level Percentage of deduplicated Percentage of total 1 79.07769130062574 18.25331849085467 2 5.767097585067464 2.6624112883589537 3 2.1616579629608816 1.4969113520100283 4 1.291109662423209 1.1920952919698713 5 0.9024239642280567 1.0415220627909265 6 0.6921410555511754 0.9585918040684103 7 0.5754524440966824 0.9298123903179835 8 0.5051439634632648 0.9328095949361302 9 0.4276908248894848 0.8885058532350085 >10 6.6617314139543415 37.536420377337684 >50 1.771035644485075 27.778351780908693 >100 0.16346851970622917 5.05656139701912 >500 0.0026365888595370696 0.38087146757681 >1k 4.7937979264310354E-4 0.19862413837895818 >5k 0.0 0.0 >10k+ 2.3968989632155177E-4 0.6931927102367595 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12529 0.6890384643939965 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2531 0.13919357916682937 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03640701280459938 0.0 2 0.0 0.0 0.0 0.12753453579133833 0.0 3 0.0 0.0 0.0 0.19292417057180458 0.0 4 0.0 0.0 0.0 0.29010119719677 0.0 5 0.0 0.0 0.0 0.4713113289054633 0.0 6 0.0 0.0 0.0 0.8000193584116423 0.0 7 0.0 0.0 0.0 1.0138418142791383 0.0 8 0.0 0.0 0.0 1.6138975797035853 0.0 9 0.0 0.0 0.0 1.9505799549146992 0.0 10 0.0 0.0 0.0 2.3877940814956133 0.0 11 0.0 0.0 0.0 2.640333360647759 0.0 12 0.0 0.0 0.0 2.861910180269709 0.0 13 0.0 0.0 0.0 2.9602421121346993 0.0 14 0.0 0.0 0.0 3.0080881863643087 0.0 15 0.0 0.0 0.0 3.0549993373043742 0.0 16 0.0 0.0 0.0 3.14315710395962 0.0 17 0.0 0.0 0.0 3.2441838147180024 0.0 18 0.0 0.0 0.0 3.3928366177555134 0.0 19 0.0 0.0 0.0 3.464330751661826 0.0 20 0.0 0.0 0.0 3.5547983287971223 0.0 21 0.0 0.0 0.0 3.6519753554220875 0.0 22 0.0 0.0 0.0 3.7559718225119627 0.0 23 0.0 0.0 0.0 3.868877558596317 0.0 24 0.0 0.0 0.0 3.961819932674524 0.0 25 0.0 0.0 0.0 4.039748538632405 0.0 26 0.0 0.0 0.0 4.112122600340642 0.0 27 0.0 0.0 0.0 4.1976405835901165 0.0 28 0.0 0.0 0.0 4.27842895490425 0.0 29 0.0 0.0 0.0 4.3719762793462795 0.0 30 0.0 0.0 0.0 4.502425575981491 0.0 31 0.0 0.0 0.0 4.605707101732303 0.0 32 0.0 0.0 0.0 4.710418510161241 0.0 33 0.0 0.0 0.0 4.812655121647269 0.0 34 0.0 0.0 0.0 4.926055817120205 0.0 35 0.0 0.0 0.0 5.057110064119239 0.0 36 0.0 0.0 0.0 5.160776558283393 0.0 37 0.0 0.0 0.0 5.278356910815468 0.0 38 0.0 0.0 0.0 5.4219501289919165 0.0 39 0.0 0.0 0.0 5.652986172484548 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 50 2.1827873E-11 45.0 1 TGTTACG 85 0.0 45.0 1 CGTTTTT 9030 0.0 43.48007 1 CGACGGT 195 0.0 40.384617 28 TTACGGG 465 0.0 39.67742 3 ATTAGCG 125 0.0 39.600002 1 GTTACGG 165 0.0 38.181816 2 CGGTCTA 210 0.0 37.5 31 TACGGGA 385 0.0 37.4026 4 GCGCGAC 740 0.0 37.094593 9 GTTTTTT 11255 0.0 36.92359 2 CGAACTA 25 0.0021074305 36.0 24 TATGCGA 25 0.0021074305 36.0 1 TAGGGAC 1205 0.0 35.850624 5 GTAGGGT 880 0.0 35.795456 4 CGTCATA 670 0.0 35.59701 38 TAGGGTA 875 0.0 35.485714 5 TAGACGG 260 0.0 35.48077 2 CGTATGG 235 0.0 35.425533 2 TTGTAGG 1090 0.0 35.29817 2 >>END_MODULE