FastQCFastQC Report
Thu 26 May 2016
SRR1546302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546302_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2723872
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194500.7140570482019712No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61900.2272500323069513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA406.8212103E-945.040
CGTTTTT180300.043.9018331
ACATACG3100.042.0967717
CGGTCTA2100.041.78571731
CGTAAGG4950.040.4545442
GCGTTAG1900.040.263161
AGGGCGA30000.038.556
TACGGGA10050.038.507464
CATACGA3400.038.3823518
ACACGAC3100.037.74193626
TAACGGG7300.037.602743
ACGGGAT14250.037.578955
CGTTAGG4500.037.5000042
CGCATTA301.1403329E-437.49999631
GTTTTTT218500.037.4519462
TAGGGCG11100.037.29735
ACGGACG1450.037.241381
TAGTGCG1450.037.241381
GACACGA3150.037.14285725
GACCGAT16150.037.0588239
Produced by FastQC (version 0.11.5)