##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546302_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2723872
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13543734801048	31.0	31.0	33.0	30.0	34.0
2	31.46659497949977	31.0	31.0	34.0	30.0	34.0
3	31.46959585472445	31.0	31.0	34.0	30.0	34.0
4	35.35421781933953	37.0	35.0	37.0	33.0	37.0
5	35.28894162427603	37.0	35.0	37.0	33.0	37.0
6	35.33362727764006	37.0	35.0	37.0	33.0	37.0
7	35.82043466065953	37.0	35.0	37.0	35.0	37.0
8	35.739294284019216	37.0	35.0	37.0	35.0	37.0
9	37.48472615453296	39.0	37.0	39.0	35.0	39.0
10	36.93184738489914	39.0	37.0	39.0	32.0	39.0
11	36.623170986008155	39.0	35.0	39.0	32.0	39.0
12	36.10769999471341	38.0	35.0	39.0	32.0	39.0
13	35.88853404271566	38.0	35.0	39.0	31.0	39.0
14	36.857866302087615	39.0	35.0	41.0	31.0	41.0
15	37.08378954664536	39.0	35.0	41.0	32.0	41.0
16	37.140446761081286	39.0	35.0	41.0	32.0	41.0
17	37.056666025422636	38.0	35.0	41.0	32.0	41.0
18	36.92219568320391	38.0	35.0	40.0	32.0	41.0
19	36.7972478148753	38.0	35.0	40.0	31.0	41.0
20	36.617019448784674	38.0	35.0	40.0	31.0	41.0
21	36.47483582194758	38.0	35.0	40.0	31.0	41.0
22	36.380964670880275	38.0	35.0	40.0	31.0	41.0
23	36.29299027267067	38.0	35.0	40.0	31.0	41.0
24	36.25195126643249	38.0	35.0	40.0	31.0	41.0
25	36.21889281140964	38.0	35.0	40.0	31.0	41.0
26	36.11467021945231	38.0	35.0	40.0	30.0	41.0
27	35.993254822546724	38.0	35.0	40.0	30.0	41.0
28	35.95880386449877	38.0	35.0	40.0	30.0	41.0
29	35.890824899261055	38.0	35.0	40.0	30.0	41.0
30	35.7045562346542	37.0	34.0	40.0	29.0	41.0
31	35.4822399877821	37.0	34.0	40.0	29.0	41.0
32	35.24667568813806	37.0	34.0	40.0	27.0	41.0
33	34.96163953372258	37.0	34.0	40.0	25.0	41.0
34	34.68942373209901	37.0	34.0	40.0	24.0	41.0
35	34.425474471634494	37.0	33.0	40.0	23.0	41.0
36	34.22343120381574	37.0	33.0	40.0	22.0	41.0
37	34.02784749063098	37.0	33.0	40.0	22.0	41.0
38	33.953045150432914	37.0	33.0	40.0	22.0	41.0
39	33.894008602460026	37.0	33.0	40.0	21.0	41.0
40	33.73262363282856	36.0	33.0	40.0	20.0	41.0
41	33.64196041517369	36.0	33.0	40.0	20.0	41.0
42	33.53939282022063	36.0	33.0	40.0	19.0	41.0
43	33.50670112251971	36.0	33.0	40.0	19.0	41.0
44	33.30049649910128	36.0	32.0	40.0	18.0	41.0
45	33.271928710306504	36.0	32.0	40.0	18.0	41.0
46	33.200085760270674	35.0	32.0	40.0	18.0	41.0
47	33.13431468145346	35.0	32.0	40.0	18.0	41.0
48	33.050730357373624	35.0	32.0	40.0	18.0	41.0
49	33.06478865379871	36.0	32.0	40.0	18.0	41.0
50	32.92057629727094	35.0	32.0	39.0	18.0	41.0
51	32.701332147766124	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	2.0
12	6.0
13	3.0
14	44.0
15	110.0
16	311.0
17	808.0
18	1899.0
19	3568.0
20	6372.0
21	10118.0
22	15228.0
23	22450.0
24	32450.0
25	47042.0
26	59487.0
27	65028.0
28	66199.0
29	70086.0
30	79868.0
31	95380.0
32	115885.0
33	147594.0
34	233256.0
35	308583.0
36	205052.0
37	254596.0
38	367097.0
39	515297.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.892467781158583	23.468136535050103	28.505157364222693	16.134238319568613
2	33.26041018080145	24.883768400277255	29.169909599276323	12.685911819644977
3	27.2143478107635	24.662722771114062	34.848186698934455	13.274742719187978
4	24.25705025786821	27.60717096838618	33.76729890391325	14.368479869832356
5	22.113484040366068	31.4429606090154	32.57862337143595	13.864931979182577
6	19.825454353214838	39.612691051561896	29.91473167608463	10.647122919138639
7	84.77758866789628	3.7887242866037756	9.688854689207128	1.7448323562928068
8	85.71687656395014	3.048270990707346	9.20527836844022	2.0295740769022923
9	80.69876264376593	5.398087722183715	11.111315069136877	2.791834564913476
10	42.028810458053826	31.28116886549735	16.18082641181377	10.509194264635049
11	34.499675461989405	22.73307996851541	28.399352098777037	14.367892470718155
12	31.84297940578706	22.058966060079182	30.327636540924097	15.770417993209666
13	21.3456799732146	32.77301576579222	30.74689265868587	15.134411602307305
14	16.882254379060395	35.45085819010585	31.784349631700753	15.882537799132997
15	15.963892576449995	24.177898227229473	44.281596198352936	15.576612997967597
16	18.387868446094384	21.82554833707311	41.718370026197995	18.06821319063451
17	18.362573571739055	21.440508217713607	32.048569095757806	28.148349114789532
18	21.685673923003723	23.487116868927764	35.11622425723382	19.710984950834696
19	26.115213930757392	24.827378085313846	29.148212544495482	19.909195439433276
20	27.693408500839983	23.859821606889017	28.64455451651179	19.802215375759214
21	22.689392159396622	26.87894291655408	30.679011348550887	19.752653575498407
22	22.810322952032987	24.195850612657278	29.41136734765804	23.582459087651696
23	19.545264975740416	29.249355329472166	29.0020970148377	22.20328267994972
24	20.047895055274257	25.13888317806417	36.89358383947557	17.919637927186006
25	18.435447774344755	26.667736222553778	34.39930363835011	20.497512364751355
26	18.73366296213625	30.672072696514373	30.512887536565596	20.08137680478378
27	18.704770268206435	29.707416501215917	31.265235664524617	20.32257756605303
28	16.993970348092716	27.59377092609344	36.52410979664243	18.888148929171415
29	18.453253309994007	24.26901851482008	36.15419520447363	21.12353297071228
30	19.625958929053937	27.846132270532536	32.814133703786375	19.713775096627153
31	24.8110410474501	26.69369192091258	28.404051291690653	20.091215739946662
32	25.83105960926211	26.915361661634613	28.383675884916766	18.869902844186512
33	24.641980239893797	27.025278720879687	27.59927779278909	20.733463246437424
34	20.65717478648042	26.70613743964474	30.694100163296955	21.94258761057788
35	20.60911819644976	26.822258902033575	30.861729185512388	21.706893716004277
36	25.861824637868448	26.4269025857309	27.8811192302722	19.83015354612845
37	21.539081131565656	29.490592803186054	29.757015013921357	19.213311051326933
38	21.36631236710095	30.037865215399258	26.979351452638014	21.616470964861783
39	21.033771043573264	29.775040824238435	28.583208021522307	20.607980110665995
40	22.68282063180649	26.249251066129393	28.07841190775484	22.989516394309277
41	18.894353332315177	26.2314088180355	28.282092550604432	26.59214529904489
42	21.539227981344204	27.238467886890426	26.330826118114214	24.891478013651156
43	22.031615288824145	26.811392018420836	28.04786715381633	23.109125538938688
44	21.12059597514127	29.69757756605303	28.158591886843436	21.023234571962266
45	19.2206902526991	31.532906098377605	26.105081296037408	23.141322352885894
46	22.097991388728985	29.924276911690416	27.260458641228368	20.71727305835223
47	21.656157117515068	27.46307462318347	28.592753257128088	22.288015002173378
48	22.42234583710248	25.258896159584594	29.581713090776656	22.737044912536273
49	21.161016376687304	25.06498102700861	30.66983323739148	23.104169358912607
50	19.613219710764675	30.01326787749204	28.550166821348434	21.82334559039485
51	19.257329272447457	30.277781041106188	26.85104145862948	23.613848227816874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1218.0
1	1799.0
2	2380.0
3	18582.0
4	34784.0
5	25859.0
6	16934.0
7	17266.0
8	17598.0
9	18495.5
10	19393.0
11	19392.5
12	19392.0
13	19392.0
14	19392.0
15	18703.0
16	18014.0
17	17630.0
18	17246.0
19	16509.0
20	15772.0
21	15992.0
22	16212.0
23	17080.5
24	17949.0
25	18004.5
26	22005.0
27	25950.0
28	28946.0
29	31942.0
30	37354.5
31	42767.0
32	51333.5
33	59900.0
34	68814.5
35	77729.0
36	84249.0
37	90769.0
38	106201.0
39	121633.0
40	148377.5
41	175122.0
42	196349.5
43	217577.0
44	230461.5
45	243346.0
46	244441.0
47	245536.0
48	236530.5
49	227525.0
50	216080.0
51	204635.0
52	184399.0
53	164163.0
54	149932.0
55	135701.0
56	121950.0
57	108199.0
58	101674.0
59	95149.0
60	82515.0
61	69881.0
62	62348.5
63	54816.0
64	45197.5
65	35579.0
66	28071.5
67	20564.0
68	17303.0
69	14042.0
70	12418.0
71	10794.0
72	9294.0
73	7794.0
74	5632.5
75	2913.0
76	2355.0
77	1814.5
78	1274.0
79	1011.0
80	748.0
81	556.0
82	364.0
83	230.0
84	96.0
85	77.5
86	59.0
87	40.5
88	22.0
89	17.5
90	13.0
91	11.0
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2723872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.626497646291803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78589948168832	18.052754668296952
2	7.338151369787487	3.320733293932591
3	2.54065820674721	1.7245859080499315
4	1.3133732899851327	1.1886815061820453
5	0.8560913559456294	0.968517452515727
6	0.6212410962026581	0.843390612060551
7	0.5027012919048544	0.7962058718651126
8	0.3983053938385488	0.7209804844954603
9	0.3361222596503097	0.6844742565159613
>10	4.0627667701492465	21.444479978941576
>50	1.4145622335633279	23.1762263766082
>100	0.8278259403622168	25.785312993652305
>500	0.0016437929822013505	0.24067770221476367
>1k	3.287585964402701E-4	0.09934324565223825
>5k	1.6437929822013506E-4	0.23022639108474532
>10k+	1.6437929822013506E-4	0.7234092579318735
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19450	0.7140570482019712	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6190	0.2272500323069513	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05014919937500734	0.0
2	0.0	0.0	0.0	0.17368657558064401	0.0
3	0.0	0.0	0.0	0.2645131636141493	0.0
4	0.0	0.0	0.0	0.4098944443791779	0.0
5	0.0	0.0	0.0	0.7138734859787832	0.0
6	0.0	0.0	0.0	1.1536885727376323	0.0
7	0.0	0.0	0.0	1.431785340867706	0.0
8	0.0	0.0	0.0	2.156378860680678	0.0
9	0.0	0.0	0.0	2.5431077524935093	0.0
10	0.0	0.0	0.0	3.108075563022051	0.0
11	0.0	0.0	0.0	3.5140050633803646	0.0
12	0.0	0.0	0.0	3.882487870208292	0.0
13	0.0	0.0	0.0	4.043398515055039	0.0
14	0.0	0.0	0.0	4.112528048307703	0.0
15	0.0	0.0	0.0	4.19480063674064	0.0
16	0.0	0.0	0.0	4.364999530080708	0.0
17	0.0	0.0	0.0	4.575104850741885	0.0
18	0.0	0.0	0.0	4.8726225020852665	0.0
19	0.0	0.0	0.0	5.012643765933201	0.0
20	0.0	0.0	0.0	5.180236075703998	0.0
21	0.0	0.0	0.0	5.36728598113274	0.0
22	0.0	1.1013733391290046E-4	0.0	5.56788277863277	0.0
23	0.0	1.1013733391290046E-4	0.0	5.795646785164648	0.0
24	0.0	1.1013733391290046E-4	0.0	5.96812185007225	0.0
25	0.0	1.1013733391290046E-4	0.0	6.10898750014685	0.0
26	0.0	1.1013733391290046E-4	0.0	6.249265751107247	0.0
27	0.0	1.1013733391290046E-4	0.0	6.396592791438071	0.0
28	0.0	1.1013733391290046E-4	0.0	6.5586415220685845	0.0
29	0.0	1.1013733391290046E-4	0.0	6.732107822981403	0.0
30	0.0	1.1013733391290046E-4	0.0	6.958072919726037	0.0
31	0.0	1.1013733391290046E-4	0.0	7.147949683391878	0.0
32	0.0	1.1013733391290046E-4	0.0	7.327583675003818	0.0
33	0.0	1.1013733391290046E-4	0.0	7.506079580831992	0.0
34	0.0	1.1013733391290046E-4	0.0	7.699333889404495	0.0
35	0.0	1.1013733391290046E-4	0.0	7.916634849214647	0.0
36	0.0	1.1013733391290046E-4	0.0	8.112091904465409	0.0
37	0.0	1.1013733391290046E-4	0.0	8.30567662503965	0.0
38	0.0	1.1013733391290046E-4	0.0	8.503593414081132	0.0
39	0.0	1.1013733391290046E-4	0.0	8.703969936913335	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCTA	40	6.8212103E-9	45.0	40
CGTTTTT	18030	0.0	43.901833	1
ACATACG	310	0.0	42.09677	17
CGGTCTA	210	0.0	41.785717	31
CGTAAGG	495	0.0	40.454544	2
GCGTTAG	190	0.0	40.26316	1
AGGGCGA	3000	0.0	38.55	6
TACGGGA	1005	0.0	38.50746	4
CATACGA	340	0.0	38.38235	18
ACACGAC	310	0.0	37.741936	26
TAACGGG	730	0.0	37.60274	3
ACGGGAT	1425	0.0	37.57895	5
CGTTAGG	450	0.0	37.500004	2
CGCATTA	30	1.1403329E-4	37.499996	31
GTTTTTT	21850	0.0	37.451946	2
TAGGGCG	1110	0.0	37.2973	5
ACGGACG	145	0.0	37.24138	1
TAGTGCG	145	0.0	37.24138	1
GACACGA	315	0.0	37.142857	25
GACCGAT	1615	0.0	37.058823	9
>>END_MODULE