FastQCFastQC Report
Thu 26 May 2016
SRR1546301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546301_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2097900
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105970.5051241717908385No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC101720.48486581819915153No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23340.11125411125411126No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT253.8919097E-545.00000414
GTACCGG453.8562575E-1045.00000424
TCCGCAT302.1663618E-645.00000443
CACGGAT207.034618E-445.00000423
TCCGATT302.1663618E-645.00000410
CGTTGCA302.1663618E-645.00000424
TTACTCG253.8919097E-545.00000444
CGTCTAC207.034618E-445.00000416
ATTCGAC207.034618E-445.00000414
CAATTCG207.034618E-445.0000041
TGACGTT302.1663618E-645.00000421
TAACGAA253.8919097E-545.00000419
CGACAAA207.034618E-445.00000431
TCTAGCG900.045.0000041
CGTTACA207.034618E-445.00000445
TAGCACG1100.045.0000041
ACTAGTC406.8193913E-945.00000410
TCTACGT253.8919097E-545.00000435
CGTACGT253.8919097E-545.00000442
TACTCGA253.8919097E-545.00000445