FastQCFastQC Report
Thu 26 May 2016
SRR1546299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546299_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4249051
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380810.8962236508811027No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT62660.14746822290436148No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC54150.12744022135766317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT239300.043.5144161
CGGTCTA5750.041.4782631
CGAACTA451.9304935E-840.00000432
CGAATAT7300.037.60273714
ATATACG900.037.5000041
TCACGAC6500.037.38461725
GTAGGGC18800.037.2207454
CTCACGA6600.037.15909224
GTTTTTT291850.037.151792
CGACGGT6400.036.91406628
ACGGGTA4450.036.910115
GCTACGA7500.036.89999810
CGTAAGG6900.036.8478282
CACGACG6500.036.69230726
ATTATAC28800.036.4843842
TAGGGTA22000.036.4090925
AGGGCGA50350.036.3753746
TACACGG2850.036.315792
AACGGGA7500.036.34
ACGGGAT9200.036.1956565
Produced by FastQC (version 0.11.5)