##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546299_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 4249051 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.158652602663512 31.0 31.0 33.0 30.0 34.0 2 31.49598769231059 31.0 31.0 34.0 30.0 34.0 3 31.538403045762454 31.0 31.0 34.0 30.0 34.0 4 35.424861927992865 37.0 35.0 37.0 33.0 37.0 5 35.352039078843724 37.0 35.0 37.0 33.0 37.0 6 35.39032198013156 37.0 35.0 37.0 33.0 37.0 7 35.791837283195704 37.0 35.0 37.0 35.0 37.0 8 35.68562344862418 37.0 35.0 37.0 35.0 37.0 9 37.4199029383267 39.0 37.0 39.0 35.0 39.0 10 36.94215508357043 39.0 37.0 39.0 32.0 39.0 11 36.68584396845319 39.0 35.0 39.0 32.0 39.0 12 36.266254511889834 38.0 35.0 39.0 32.0 39.0 13 36.080636123219044 38.0 35.0 39.0 32.0 39.0 14 37.038549549064015 39.0 35.0 41.0 31.0 41.0 15 37.220872142979694 39.0 35.0 41.0 32.0 41.0 16 37.25035872716049 39.0 35.0 41.0 32.0 41.0 17 37.164174070869 39.0 35.0 41.0 32.0 41.0 18 37.058420574382374 39.0 35.0 40.0 32.0 41.0 19 36.93560138487394 38.0 35.0 40.0 31.0 41.0 20 36.772402590602 38.0 35.0 40.0 31.0 41.0 21 36.620897230934624 38.0 35.0 40.0 31.0 41.0 22 36.534557245841484 38.0 35.0 40.0 31.0 41.0 23 36.46007732079469 38.0 35.0 40.0 31.0 41.0 24 36.42439547089456 38.0 35.0 40.0 31.0 41.0 25 36.35658715322551 38.0 35.0 40.0 30.0 41.0 26 36.25587972467264 38.0 35.0 40.0 30.0 41.0 27 36.144721727275105 38.0 35.0 40.0 30.0 41.0 28 36.07634975433338 38.0 35.0 40.0 30.0 41.0 29 35.94995729634688 38.0 35.0 40.0 30.0 41.0 30 35.75054688682249 38.0 34.0 40.0 29.0 41.0 31 35.54116860447191 38.0 34.0 40.0 28.0 41.0 32 35.3518559791351 38.0 34.0 40.0 27.0 41.0 33 35.12397756581411 38.0 34.0 40.0 26.0 41.0 34 34.83077044733047 37.0 34.0 40.0 24.0 41.0 35 34.58709933112123 37.0 33.0 40.0 23.0 41.0 36 34.370556390120996 37.0 33.0 40.0 23.0 41.0 37 34.16479468003561 37.0 33.0 40.0 23.0 41.0 38 34.05039972455026 37.0 33.0 40.0 22.0 41.0 39 33.951417622429105 37.0 33.0 40.0 22.0 41.0 40 33.74312546495676 36.0 33.0 40.0 21.0 41.0 41 33.60769334140729 36.0 33.0 40.0 20.0 41.0 42 33.499789482404424 36.0 32.0 40.0 20.0 41.0 43 33.462480210286955 36.0 32.0 40.0 20.0 41.0 44 33.26138895485133 35.0 32.0 40.0 19.0 41.0 45 33.2098216754753 35.0 32.0 40.0 20.0 41.0 46 33.107539542358985 35.0 32.0 39.0 20.0 41.0 47 33.02033254013661 35.0 32.0 39.0 20.0 41.0 48 32.90599901013191 35.0 31.0 39.0 19.0 41.0 49 32.88644758559029 35.0 31.0 39.0 19.0 41.0 50 32.744520600011626 35.0 31.0 39.0 18.0 41.0 51 32.52590825574934 35.0 31.0 39.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 9.0 10 10.0 11 13.0 12 18.0 13 42.0 14 99.0 15 226.0 16 571.0 17 1409.0 18 3030.0 19 5860.0 20 10018.0 21 15774.0 22 23798.0 23 33373.0 24 47439.0 25 66337.0 26 86006.0 27 97043.0 28 102001.0 29 110882.0 30 127067.0 31 150233.0 32 182793.0 33 230801.0 34 352119.0 35 444461.0 36 353332.0 37 442679.0 38 599637.0 39 761862.0 40 107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.42207471738984 22.505637141093388 27.465944748603867 16.606343392912912 2 33.8192457562877 24.2744085679367 28.998616396931926 12.907729278843677 3 28.68494635625696 25.093532649996437 32.80377194813619 13.41774904561042 4 25.746760864955494 27.33838685391162 31.864856411466935 15.049995869665956 5 22.653670196003766 31.05243970947866 31.533229419934006 14.760660674583573 6 20.690078796418305 39.81350188547984 28.770824355838513 10.725594962263338 7 84.3915264843844 4.197807934053981 9.297746720385328 2.1129188611762957 8 84.74360510146855 3.633776106711828 9.075673603352843 2.5469451884667893 9 79.56160093159626 5.245712513217658 11.491554231756691 3.7011323234293965 10 42.606807967237856 24.507919533091034 18.154571456073366 14.730701043597735 11 34.3514116446237 24.06993938175842 25.10313479409873 16.475514179519145 12 30.82923692843414 20.873272643703263 29.92204612276953 18.375444305093065 13 23.816776969728064 27.30002534683627 30.325265571065163 18.557932112370505 14 20.19177929377642 29.612447579471272 31.864079767458662 18.33169335929364 15 19.360064164915883 22.60831889285396 39.014523478301385 19.01709346392877 16 21.878603010413382 19.80282185363273 36.47381497656771 21.84476015938618 17 21.313088499055436 20.861622983579156 30.529805361244193 27.295483156121215 18 23.840193963310867 21.80903453500558 32.89350963309219 21.457261868591363 19 27.30153156551898 24.697444205776772 27.0161266598118 20.984897568892443 20 28.9353787469249 23.319936616435058 26.746466446272354 20.998218190367684 21 24.987132420862917 25.83748700592203 28.807844386899568 20.367536186315487 22 24.595539098024478 22.460332907277415 27.770648081183303 25.173479913514807 23 22.904220260006294 25.803079322888806 26.99247431955983 24.300226097545075 24 22.668120481491044 24.013197299820597 33.1252319635608 20.19345025512756 25 22.80163264691339 24.00168884769799 30.21975965927451 22.97691884611411 26 20.82210827782486 26.67054361079686 28.707986795169088 23.799361316209197 27 21.40623871071446 25.16326586807266 29.511319115727254 23.919176305485625 28 20.33529369263866 25.155734774659095 32.47878173267396 22.03018980002829 29 21.27347965463347 23.41045094539934 31.888249870382822 23.42781952958437 30 23.05182969091216 25.12059751695143 30.65413900656876 21.173433785567646 31 26.58857236592359 24.431196518940347 27.229421346084102 21.75080976905196 32 26.740418036874587 24.00286558104386 27.828849312470005 21.427867069611544 33 26.30632110558334 25.148368423913954 26.61653155022145 21.928778920281257 34 22.370595222321406 25.150674821271856 29.676461873486577 22.802268082920165 35 22.555648308292838 25.836945708582924 29.415650694708066 22.191755288416164 36 27.216500813946453 26.076552152468867 25.874224620980073 20.832722412604603 37 23.497646886328265 28.427265288178464 26.636700759769656 21.438387065723617 38 24.182646901625798 28.348871312676643 25.43450290429557 22.03397888140199 39 23.503059859719265 26.911609204031677 25.36924127293365 24.216089663315408 40 25.13215303840787 24.085707608593072 27.16794879609588 23.61419055690318 41 21.146227710611146 23.645585802570974 27.71922483396881 27.48896165284907 42 22.873295707676846 25.162089134726788 26.08933147660501 25.875283680991355 43 22.604835762150184 24.692125371053443 27.406025486632192 25.297013380164184 44 23.069245344431028 25.722520158030584 28.207686845839223 23.00054765169917 45 21.70997712195029 27.47164013799787 26.92655371752422 23.89182902252762 46 23.33937625130882 26.461249817900516 27.666177694737016 22.53319623605365 47 22.992569399614172 25.578017303157814 28.297612808130566 23.131800489097447 48 23.040956674796327 24.861033675519543 28.95011144841519 23.147898201268944 49 22.866400050270048 23.803597556254328 29.217935957935076 24.112066435540548 50 21.94708889114299 26.314605308338262 28.434043272250676 23.304262528268076 51 21.57314656849259 27.3464827793312 27.155263610627404 23.925107041548806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2169.0 1 3855.0 2 5541.0 3 35537.5 4 65534.0 5 45631.5 6 25729.0 7 25461.0 8 25193.0 9 25570.5 10 25948.0 11 25608.0 12 25268.0 13 24337.0 14 23406.0 15 22659.0 16 21912.0 17 20696.5 18 19481.0 19 19353.0 20 19225.0 21 18986.5 22 18748.0 23 18635.0 24 18522.0 25 20860.5 26 26932.0 27 30665.0 28 35609.5 29 40554.0 30 46529.5 31 52505.0 32 63538.0 33 74571.0 34 89936.0 35 105301.0 36 106443.5 37 107586.0 38 120502.5 39 133419.0 40 162257.5 41 191096.0 42 225257.5 43 259419.0 44 275375.5 45 291332.0 46 304371.5 47 317411.0 48 306063.5 49 294716.0 50 285615.0 51 276514.0 52 264133.0 53 251752.0 54 246744.0 55 241736.0 56 241871.5 57 242007.0 58 228459.0 59 214911.0 60 202362.0 61 189813.0 62 177306.0 63 164799.0 64 150839.0 65 136879.0 66 119702.5 67 102526.0 68 87101.0 69 71676.0 70 60302.0 71 48928.0 72 43621.0 73 38314.0 74 30441.5 75 18113.5 76 13658.0 77 10300.5 78 6943.0 79 5222.0 80 3501.0 81 2802.5 82 2104.0 83 1608.0 84 1112.0 85 794.5 86 477.0 87 326.5 88 176.0 89 140.0 90 104.0 91 77.0 92 50.0 93 39.5 94 29.0 95 24.5 96 20.0 97 11.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4249051.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.2786619209826 #Duplication Level Percentage of deduplicated Percentage of total 1 79.97139178645834 16.217128173880337 2 7.076224599098848 2.869927326441323 3 2.4466150961428057 1.488422411563569 4 1.2922758401304508 1.0482249948263667 5 0.8281982347888522 0.839737600341885 6 0.5844074694275152 0.7110600897970532 7 0.4739706576066188 0.6728043508249295 8 0.39342732801067903 0.638254382016327 9 0.3183828785917817 0.581074088075279 >10 4.098653664623866 19.91879068322174 >50 1.4331643764475117 21.001592504241838 >100 1.07481248964235 31.3170297319177 >500 0.006003535146530627 0.8089269395597435 >1k 0.0021188947575988683 0.6991428041970145 >5k 2.354327508443187E-4 0.2788407230205315 >10k+ 1.1771637542215935E-4 0.9090431960743822 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38081 0.8962236508811027 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 6266 0.14746822290436148 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 5415 0.12744022135766317 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3534666917389317E-5 0.0 0.0 0.0420799844482921 0.0 2 2.3534666917389317E-5 0.0 0.0 0.14050196149681424 0.0 3 2.3534666917389317E-5 0.0 0.0 0.21741325298284253 0.0 4 2.3534666917389317E-5 0.0 0.0 0.33680461825475855 0.0 5 2.3534666917389317E-5 0.0 0.0 0.5782467661602555 0.0 6 2.3534666917389317E-5 0.0 0.0 1.06211951798178 0.0 7 2.3534666917389317E-5 0.0 0.0 1.3709178826048452 0.0 8 2.3534666917389317E-5 0.0 0.0 2.1539397856133053 0.0 9 2.3534666917389317E-5 0.0 0.0 2.6255980453047045 0.0 10 2.3534666917389317E-5 0.0 0.0 3.272330692194563 0.0 11 2.3534666917389317E-5 0.0 0.0 3.663500391028491 0.0 12 2.3534666917389317E-5 0.0 0.0 3.9927503812027676 0.0 13 2.3534666917389317E-5 0.0 0.0 4.140265673440964 0.0 14 2.3534666917389317E-5 0.0 0.0 4.2081631874976315 0.0 15 2.3534666917389317E-5 0.0 0.0 4.276907949563326 0.0 16 2.3534666917389317E-5 0.0 0.0 4.413126601681175 0.0 17 2.3534666917389317E-5 0.0 0.0 4.556970485880259 0.0 18 2.3534666917389317E-5 0.0 0.0 4.778102216236049 0.0 19 2.3534666917389317E-5 0.0 0.0 4.878383431971045 0.0 20 2.3534666917389317E-5 0.0 0.0 4.992244150517374 0.0 21 2.3534666917389317E-5 0.0 0.0 5.125167949266789 0.0 22 2.3534666917389317E-5 0.0 0.0 5.263245840071113 0.0 23 2.3534666917389317E-5 0.0 0.0 5.41796274038603 0.0 24 2.3534666917389317E-5 0.0 0.0 5.543096564385789 0.0 25 2.3534666917389317E-5 0.0 0.0 5.646225474817789 0.0 26 2.3534666917389317E-5 0.0 0.0 5.74591832387985 0.0 27 2.3534666917389317E-5 0.0 0.0 5.8530951970216405 0.0 28 2.3534666917389317E-5 0.0 0.0 5.961778288846145 0.0 29 2.3534666917389317E-5 0.0 0.0 6.083452516809047 0.0 30 2.3534666917389317E-5 0.0 0.0 6.249630799912733 0.0 31 2.3534666917389317E-5 0.0 0.0 6.37631791192904 0.0 32 2.3534666917389317E-5 0.0 0.0 6.5092652453453725 0.0 33 2.3534666917389317E-5 0.0 0.0 6.641812489424109 0.0 34 2.3534666917389317E-5 0.0 0.0 6.777066220198345 0.0 35 2.3534666917389317E-5 0.0 0.0 6.94145586861631 0.0 36 2.3534666917389317E-5 0.0 0.0 7.076897876725885 0.0 37 2.3534666917389317E-5 0.0 0.0 7.220529948922713 0.0 38 4.7069333834778634E-5 0.0 0.0 7.399252209493367 0.0 39 4.7069333834778634E-5 0.0 0.0 7.661263656284662 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTTT 23930 0.0 43.514416 1 CGGTCTA 575 0.0 41.47826 31 CGAACTA 45 1.9304935E-8 40.000004 32 CGAATAT 730 0.0 37.602737 14 ATATACG 90 0.0 37.500004 1 TCACGAC 650 0.0 37.384617 25 GTAGGGC 1880 0.0 37.220745 4 CTCACGA 660 0.0 37.159092 24 GTTTTTT 29185 0.0 37.15179 2 CGACGGT 640 0.0 36.914066 28 ACGGGTA 445 0.0 36.91011 5 GCTACGA 750 0.0 36.899998 10 CGTAAGG 690 0.0 36.847828 2 CACGACG 650 0.0 36.692307 26 ATTATAC 2880 0.0 36.48438 42 TAGGGTA 2200 0.0 36.409092 5 AGGGCGA 5035 0.0 36.375374 6 TACACGG 285 0.0 36.31579 2 AACGGGA 750 0.0 36.3 4 ACGGGAT 920 0.0 36.195656 5 >>END_MODULE