FastQCFastQC Report
Thu 26 May 2016
SRR1546298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546298_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2502962
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC135710.5421976042784509No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103160.4121516826863532No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29290.11702135310084612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG750.045.0000041
TACCCGT302.166531E-645.00000441
GTCGATA302.166531E-645.00000410
ACTTGCA302.166531E-645.00000427
ATCGGAT302.166531E-645.00000439
TAGTACG1150.045.0000041
GTGCCGA302.166531E-645.00000442
ATTTACG850.045.01
ACCGGGT351.212702E-745.029
CGAGTTA351.212702E-745.021
TTTACGA351.212702E-745.034
GGCGTAC253.8921284E-545.08
TAAGTCG253.8921284E-545.01
CGTAGAA207.0348807E-445.037
TACGAAG502.1827873E-1145.01
CGCTAAG253.8921284E-545.034
ACGTGCG406.8212103E-945.01
GTCGTGA253.8921284E-545.034
AATCTAC253.8921284E-545.013
AGCGAAC207.0348807E-445.030