##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546298_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2502962
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175428951777935	31.0	31.0	33.0	30.0	34.0
2	31.519446160189407	31.0	31.0	34.0	30.0	34.0
3	31.58182345556984	31.0	31.0	34.0	30.0	34.0
4	35.41752331837239	37.0	35.0	37.0	33.0	37.0
5	35.31478424362815	37.0	35.0	37.0	33.0	37.0
6	35.34256253191219	37.0	35.0	37.0	33.0	37.0
7	35.803918317577335	37.0	35.0	37.0	35.0	37.0
8	35.728776945075474	37.0	35.0	37.0	35.0	37.0
9	37.475737945681956	39.0	37.0	39.0	35.0	39.0
10	37.0261646001817	39.0	37.0	39.0	33.0	39.0
11	36.64378044892411	39.0	35.0	39.0	32.0	39.0
12	35.419337169321786	37.0	35.0	39.0	31.0	39.0
13	34.775658599691084	35.0	34.0	39.0	27.0	39.0
14	35.56931147975878	37.0	34.0	40.0	27.0	41.0
15	36.069718597405796	37.0	35.0	40.0	31.0	41.0
16	36.32608045987114	37.0	35.0	40.0	32.0	41.0
17	36.292246146765315	36.0	35.0	40.0	32.0	41.0
18	36.241714416759024	36.0	35.0	40.0	31.0	41.0
19	36.09528071141312	36.0	35.0	40.0	31.0	41.0
20	35.89071388219238	36.0	34.0	40.0	31.0	41.0
21	35.69637932977009	35.0	34.0	40.0	30.0	41.0
22	35.54943023505751	35.0	34.0	40.0	30.0	41.0
23	35.56782324302167	35.0	34.0	40.0	30.0	41.0
24	35.52631921699171	35.0	34.0	40.0	30.0	41.0
25	35.45384548387071	35.0	34.0	40.0	30.0	41.0
26	35.32333531232196	35.0	34.0	40.0	30.0	41.0
27	35.174665855893934	35.0	34.0	40.0	29.0	41.0
28	35.18988462469666	35.0	34.0	40.0	29.0	41.0
29	35.20469867301222	36.0	34.0	40.0	30.0	41.0
30	35.09784687102721	36.0	34.0	40.0	29.0	41.0
31	34.816872169853156	35.0	34.0	40.0	29.0	41.0
32	34.53190899422364	35.0	34.0	40.0	27.0	41.0
33	34.328402908234324	35.0	33.0	40.0	26.0	41.0
34	34.14293744771195	35.0	33.0	40.0	25.0	41.0
35	33.93515402950584	35.0	33.0	39.0	24.0	41.0
36	33.67552164195861	35.0	33.0	39.0	23.0	41.0
37	33.47082097131319	35.0	33.0	39.0	23.0	41.0
38	33.42915114172728	35.0	33.0	39.0	23.0	41.0
39	33.41879461214353	35.0	33.0	39.0	23.0	41.0
40	33.15982983361314	35.0	32.0	39.0	22.0	41.0
41	33.126411028213774	35.0	32.0	39.0	21.0	41.0
42	33.00466926785145	35.0	32.0	39.0	21.0	41.0
43	32.92173273106024	35.0	32.0	39.0	21.0	41.0
44	32.712177412202024	35.0	32.0	38.0	20.0	41.0
45	32.711565736914906	35.0	32.0	38.0	20.0	40.0
46	32.665469951201814	35.0	32.0	38.0	20.0	40.0
47	32.61846084758778	35.0	32.0	38.0	20.0	40.0
48	32.56301454037256	35.0	32.0	38.0	20.0	40.0
49	32.62051960836801	35.0	32.0	38.0	20.0	40.0
50	32.429817951690836	35.0	32.0	38.0	19.0	40.0
51	32.18708154578455	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	7.0
13	9.0
14	33.0
15	87.0
16	274.0
17	750.0
18	1700.0
19	3425.0
20	5935.0
21	9560.0
22	14478.0
23	20830.0
24	29526.0
25	41162.0
26	53313.0
27	60584.0
28	64832.0
29	71129.0
30	83049.0
31	99705.0
32	124180.0
33	162554.0
34	297769.0
35	433409.0
36	153908.0
37	178673.0
38	246820.0
39	345187.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.9381444864125	23.737555743954562	29.99162592160808	15.33267384802486
2	30.235576888502504	26.317618885144878	30.21811757429797	13.228686652054646
3	27.421191372461905	24.822750005793136	33.257596399785534	14.498462221959421
4	24.846082361617956	27.527465458924265	32.6315781062597	14.994874073198075
5	22.826275428871874	31.835041842425092	31.390928028471865	13.947754700231165
6	20.350448788275653	39.97891298389668	28.54250284263205	11.12813538519562
7	84.37838848532259	5.373273745266608	8.225054954889446	2.0232828145213553
8	86.52920020359878	3.8392512551129423	7.1566008593019	2.4749476819863827
9	82.51187992466525	4.795877843930511	9.681489371392773	3.0107528600114586
10	59.301299819973295	17.50997418258847	12.994444182532536	10.194281814905699
11	53.798299774427264	17.15611343680008	18.311784198082112	10.73380259069055
12	47.200676638318924	19.961070124116947	21.260570476099915	11.577682761464217
13	20.676902006502694	46.47973081493047	21.922865788613652	10.920501389953184
14	12.737268883826442	47.73052886939554	27.570574383470465	11.961627863307553
15	10.895530974900938	20.641623804116882	56.23173663843078	12.231108582551393
16	11.988675816892146	14.769620953094773	53.502650060208666	19.739053169804414
17	13.281144499996403	16.025253279913958	29.275194749261075	41.41840747082856
18	20.436986258680715	20.94734158968454	37.02117730912415	21.594494842510596
19	30.67501624075795	22.28707427439969	25.64070089757655	21.397208587265805
20	32.69594184809838	20.98022263222534	24.245194293800704	22.078641225875582
21	21.269559825518726	29.197966249587488	27.342604482209477	22.189869442684305
22	21.839045099366274	24.84736084686863	24.072478926967328	29.241115126797766
23	17.67154275614252	31.956178319926554	24.38454918612428	25.987729737806646
24	19.234211306444124	22.44648540409323	41.56047914430982	16.758824145152822
25	14.910054567348604	23.72549003940132	39.839637996901274	21.524817396348805
26	15.237546554841824	36.002264517000256	27.971459414885242	20.788729513272674
27	16.85127460984226	34.97340351151955	28.570269944170146	19.605051934468044
28	13.238195386106542	27.467176888822127	42.4493859675057	16.845241757565635
29	12.938949932120424	23.273305787303205	41.934356174804094	21.853388105772282
30	17.179965177257987	30.55519820117125	32.07671550746675	20.18812111410401
31	30.725196786846944	24.950918152173305	23.60543228383012	20.718452777149633
32	31.51985527546962	25.787367127427423	25.27573331117292	17.417044285930032
33	28.863043066574722	25.62400068398961	25.129586465955136	20.383369783480532
34	18.157526962055357	25.885930349721647	29.184502201791318	26.772040486431674
35	19.26865050288418	23.6227717400424	33.47565804035379	23.63291971671963
36	31.805277107682816	21.262847777952683	26.479107553370767	20.452767560993735
37	20.73842910919143	30.827155985588274	29.272358110111142	19.162056795109155
38	19.675888007888254	33.1193601820563	23.565479619746522	23.639272190308922
39	19.980966550830576	31.237030366421862	27.518236393520958	21.263766689226603
40	24.6331346620524	24.416391459398902	24.69518114937422	26.255292729174474
41	16.550950433925884	22.636859848451554	26.322892636803914	34.489297080818645
42	21.864215277738936	24.976328046530472	23.619855195564295	29.5396014801663
43	22.105489416139758	25.982136364834947	25.461353388505298	26.45102083052
44	19.55690897424731	30.217158710359964	28.285846928559046	21.94008538683368
45	16.3721622621518	37.214348439968326	22.44724450471082	23.966244793169054
46	21.98319431137988	32.686233350726056	25.683290437489664	19.6472819004044
47	22.902425206615202	25.27617279047784	27.229618348181074	24.59178365472588
48	23.391965199631475	21.26995935215956	31.473590090460824	23.864485357748137
49	20.245413234399884	20.847100355498803	34.10647065356965	24.801015756531662
50	18.69361180872902	32.91120680218078	26.32265292081941	22.072528468270793
51	17.181323567836827	35.199016205599605	23.343582523426246	24.27607770313732
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1266.0
1	1724.5
2	2183.0
3	9956.0
4	17729.0
5	13716.5
6	9704.0
7	10762.5
8	11821.0
9	13070.0
10	14319.0
11	14577.5
12	14836.0
13	14226.0
14	13616.0
15	12874.0
16	12132.0
17	11850.5
18	11569.0
19	10985.0
20	10401.0
21	10671.5
22	10942.0
23	10351.5
24	9761.0
25	10556.0
26	12044.5
27	12738.0
28	15123.5
29	17509.0
30	18511.5
31	19514.0
32	26533.5
33	33553.0
34	38316.5
35	43080.0
36	48896.0
37	54712.0
38	73136.0
39	91560.0
40	137184.5
41	182809.0
42	231392.0
43	279975.0
44	282459.0
45	284943.0
46	266449.0
47	247955.0
48	235296.0
49	222637.0
50	208827.5
51	195018.0
52	172972.0
53	150926.0
54	135682.5
55	120439.0
56	109653.5
57	98868.0
58	89332.5
59	79797.0
60	73557.5
61	67318.0
62	60877.0
63	54436.0
64	44613.5
65	34791.0
66	27975.5
67	21160.0
68	17450.5
69	13741.0
70	11356.0
71	8971.0
72	7302.0
73	5633.0
74	4994.5
75	3370.0
76	2384.0
77	1811.0
78	1238.0
79	881.0
80	524.0
81	542.0
82	560.0
83	336.5
84	113.0
85	73.5
86	34.0
87	26.0
88	18.0
89	14.5
90	11.0
91	7.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2502962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.462000818103807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.200641221356	14.353876642559756
2	8.035661291839254	2.8063744810420426
3	2.705149944851024	1.4171199165024615
4	1.2807622169716044	0.8945868352221841
5	0.7143213370889157	0.6236739886317826
6	0.46022721839317043	0.48218928384571086
7	0.3175645561200292	0.38817187771381056
8	0.21728355921839046	0.3035364551065633
9	0.19083684678064547	0.29991538571471843
>10	1.5726778769104437	6.135600304950943
>50	0.5755329767235475	7.484841864205782
>100	1.7035470814933376	60.71204179911048
>500	0.023030243083653174	2.5756765152152092
>1k	0.0023030243083653175	0.5617690952119734
>5k	0.0	0.0
>10k+	4.606048616730635E-4	0.9606255549665981
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	13571	0.5421976042784509	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10316	0.4121516826863532	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2929	0.11702135310084612	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09129183743101174	0.0
2	0.0	0.0	0.0	0.38402500717150323	0.0
3	0.0	0.0	0.0	0.5249780060584219	0.0
4	0.0	0.0	0.0	0.7741627719477963	0.0
5	0.0	0.0	0.0	1.2525959243488316	0.0
6	0.0	0.0	0.0	1.778013409712173	0.0
7	0.0	0.0	0.0	2.091282248791632	0.0
8	0.0	0.0	0.0	2.897966489303473	0.0
9	0.0	0.0	0.0	3.248111637332089	0.0
10	0.0	0.0	0.0	3.814600461373365	0.0
11	0.0	0.0	0.0	4.225513611473127	0.0
12	0.0	0.0	0.0	4.638024868136232	0.0
13	0.0	0.0	0.0	4.787048305168037	0.0
14	0.0	0.0	0.0	4.839865727086548	0.0
15	0.0	0.0	0.0	4.933594677026659	0.0
16	0.0	0.0	0.0	5.139071228408581	0.0
17	0.0	0.0	0.0	5.370836632757509	0.0
18	0.0	0.0	0.0	5.761693545487307	0.0
19	0.0	0.0	0.0	5.8942964375807545	0.0
20	0.0	0.0	0.0	6.035968584421178	0.0
21	0.0	0.0	0.0	6.214996472179761	0.0
22	0.0	0.0	0.0	6.3863934010983785	0.0
23	0.0	0.0	0.0	6.600339917266023	0.0
24	0.0	0.0	0.0	6.74093334217619	0.0
25	0.0	0.0	0.0	6.848525866553308	0.0
26	0.0	0.0	0.0	6.965627124982321	0.0
27	0.0	0.0	0.0	7.071501684803844	0.0
28	0.0	0.0	0.0	7.181371511033727	0.0
29	0.0	0.0	0.0	7.298273006142322	0.0
30	0.0	0.0	0.0	7.479258574441002	0.0
31	0.0	0.0	0.0	7.648298296178687	0.0
32	0.0	0.0	0.0	7.773310182096252	0.0
33	0.0	0.0	0.0	7.897203393419477	0.0
34	0.0	0.0	0.0	8.03076514945093	0.0
35	0.0	0.0	0.0	8.231247617822405	0.0
36	0.0	0.0	0.0	8.37172118474032	0.0
37	0.0	0.0	0.0	8.510996171735728	0.0
38	0.0	0.0	0.0	8.637606164216637	0.0
39	0.0	0.0	0.0	8.766813079862978	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	75	0.0	45.000004	1
TACCCGT	30	2.166531E-6	45.000004	41
GTCGATA	30	2.166531E-6	45.000004	10
ACTTGCA	30	2.166531E-6	45.000004	27
ATCGGAT	30	2.166531E-6	45.000004	39
TAGTACG	115	0.0	45.000004	1
GTGCCGA	30	2.166531E-6	45.000004	42
ATTTACG	85	0.0	45.0	1
ACCGGGT	35	1.212702E-7	45.0	29
CGAGTTA	35	1.212702E-7	45.0	21
TTTACGA	35	1.212702E-7	45.0	34
GGCGTAC	25	3.8921284E-5	45.0	8
TAAGTCG	25	3.8921284E-5	45.0	1
CGTAGAA	20	7.0348807E-4	45.0	37
TACGAAG	50	2.1827873E-11	45.0	1
CGCTAAG	25	3.8921284E-5	45.0	34
ACGTGCG	40	6.8212103E-9	45.0	1
GTCGTGA	25	3.8921284E-5	45.0	34
AATCTAC	25	3.8921284E-5	45.0	13
AGCGAAC	20	7.0348807E-4	45.0	30
>>END_MODULE