FastQCFastQC Report
Thu 26 May 2016
SRR1546297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546297_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1891853
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79480.42011720783802964No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35430.1872767070168771No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGT302.166249E-645.00000415
ATTCGTT351.2124838E-745.00000422
GTTAGTC253.8917642E-545.00000420
CGTTCGA253.8917642E-545.00000443
TCTAGCG700.045.0000041
CGTAAGT302.166249E-645.00000416
CAGTCGA351.2124838E-745.00000442
GCTAACG600.045.0000041
CGCCCAT253.8917642E-545.00000436
ATTTCGC351.2124838E-745.00000416
CGAACTA551.8189894E-1245.040
ACGCATC207.0344453E-445.019
TCGATTG207.0344453E-445.01
ACTCGAT207.0344453E-445.027
TCGTACT406.8193913E-945.012
ATACCCG207.0344453E-445.036
GTCGGTA207.0344453E-445.01
TGCGTAG1600.045.01
TCACGAC5000.044.5525
CGACGGT4750.044.52631428