##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546297_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1891853
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189437551437663	31.0	31.0	33.0	30.0	34.0
2	31.415007402795037	31.0	31.0	34.0	30.0	34.0
3	31.504875378795287	31.0	31.0	34.0	30.0	34.0
4	35.263796394328736	37.0	35.0	37.0	33.0	37.0
5	34.971728775967264	37.0	35.0	37.0	32.0	37.0
6	35.09710056753881	37.0	35.0	37.0	32.0	37.0
7	35.68241401419667	37.0	35.0	37.0	35.0	37.0
8	35.769197712507264	37.0	35.0	37.0	35.0	37.0
9	37.508706543267365	39.0	37.0	39.0	35.0	39.0
10	36.76548600763378	39.0	37.0	39.0	32.0	39.0
11	36.44463126891994	38.0	35.0	39.0	32.0	39.0
12	35.33528397819492	37.0	35.0	39.0	30.0	39.0
13	34.7556866204721	37.0	33.0	39.0	27.0	39.0
14	35.62503957759932	37.0	34.0	40.0	27.0	41.0
15	36.00204085623989	37.0	34.0	40.0	30.0	41.0
16	36.20703934185161	37.0	35.0	40.0	31.0	41.0
17	36.15816080847719	37.0	34.0	40.0	31.0	41.0
18	36.151268623936424	37.0	35.0	40.0	31.0	41.0
19	36.06929978174837	37.0	35.0	40.0	31.0	41.0
20	35.98116238418101	36.0	34.0	40.0	31.0	41.0
21	35.83044930023633	36.0	34.0	40.0	30.0	41.0
22	35.706160045204356	36.0	34.0	40.0	30.0	41.0
23	35.70341353160103	36.0	34.0	40.0	30.0	41.0
24	35.62177769625864	36.0	34.0	40.0	30.0	41.0
25	35.48303700128921	35.0	34.0	40.0	30.0	41.0
26	35.396881258744735	35.0	34.0	40.0	29.0	41.0
27	35.30664644663195	35.0	34.0	40.0	29.0	41.0
28	35.34561829063886	36.0	34.0	40.0	29.0	41.0
29	35.39822544352019	36.0	34.0	40.0	29.0	41.0
30	35.33211089867976	36.0	34.0	40.0	29.0	41.0
31	35.17967780794808	36.0	34.0	40.0	29.0	41.0
32	35.009867574277706	35.0	34.0	40.0	29.0	41.0
33	34.83730606976335	35.0	34.0	40.0	27.0	41.0
34	34.717281416685125	35.0	34.0	40.0	27.0	41.0
35	34.570157406521545	35.0	33.0	40.0	26.0	41.0
36	34.369560425677896	35.0	33.0	40.0	25.0	41.0
37	34.269145118568936	35.0	33.0	40.0	24.0	41.0
38	34.29316654095218	35.0	33.0	40.0	25.0	41.0
39	34.24526588482298	35.0	33.0	40.0	24.0	41.0
40	34.06460068514837	35.0	33.0	40.0	23.0	41.0
41	34.04448654308765	35.0	33.0	40.0	23.0	41.0
42	33.99698496659096	35.0	33.0	40.0	24.0	41.0
43	33.917864125806815	35.0	33.0	40.0	23.0	41.0
44	33.711591228282536	35.0	33.0	39.0	23.0	41.0
45	33.716480085926335	35.0	33.0	39.0	23.0	41.0
46	33.6833707481501	35.0	33.0	39.0	23.0	41.0
47	33.642203173290945	35.0	33.0	39.0	23.0	41.0
48	33.54147917412188	35.0	33.0	39.0	23.0	41.0
49	33.497613715230514	35.0	33.0	39.0	24.0	41.0
50	33.42073459195826	35.0	32.0	39.0	24.0	41.0
51	33.330206416671906	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	9.0
14	32.0
15	86.0
16	257.0
17	637.0
18	1461.0
19	2701.0
20	4443.0
21	6917.0
22	9946.0
23	14003.0
24	19587.0
25	26143.0
26	32499.0
27	38309.0
28	43521.0
29	50736.0
30	60777.0
31	74131.0
32	92631.0
33	119576.0
34	205447.0
35	302160.0
36	123932.0
37	147734.0
38	206159.0
39	307868.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.680816109919746	24.431813676855445	29.306663889847677	13.580706323377134
2	27.84286094109849	27.386800137219964	31.315170893298795	13.455168028382754
3	28.820579611629444	25.43966153818505	31.741525372214436	13.99823347797107
4	25.120186399260408	28.9518794536362	30.576001412371888	15.351932734731506
5	23.375389102641698	32.43957115061266	29.641097907712705	14.543941839032948
6	21.171148075458294	40.45340732075907	27.460273076185093	10.915171527597547
7	86.16324841306381	5.867052038398332	5.888565337793159	2.081134210744704
8	88.0201051561617	4.499081059680641	5.231537545464684	2.249276238692964
9	84.68480373475106	5.816360996335339	6.866601157700942	2.6322341112126577
10	53.97866536142079	23.231350427332355	12.391977600796679	10.398006610450178
11	49.84055315079977	20.413161064839606	17.71046693374168	12.035818850618943
12	43.41922971816521	22.314471578922888	21.158779249762006	13.107519453149902
13	21.040006808139957	45.53012311210226	20.854791572072457	12.575078507685323
14	14.3967845281848	46.95745388251624	25.332993631111933	13.312767958187028
15	12.624765243388362	24.03833701667096	49.55887164594712	13.778026093993562
16	14.262101759491886	18.825828433815946	47.06576039470297	19.8463094119892
17	14.770756501694372	19.44596118197344	27.7971385726058	37.986143743726394
18	20.58341742196672	23.644543207109646	34.208683232788175	21.563356138135468
19	29.677094361982668	25.270938069712606	24.05054726767883	21.001420300625895
20	31.5967466816925	24.567606468367256	23.346105643514587	20.489541206425656
21	21.429254809966736	30.360445552587862	26.247599575654135	21.96270006179127
22	22.260080460796903	25.912478400805984	23.697665727728317	28.1297754106688
23	18.02867347515901	32.63504088319758	23.799153528313248	25.53713211333016
24	19.456109962031935	25.04153335380709	38.84345136752169	16.658905316639295
25	16.18910137309823	27.539718995080488	35.09189138902441	21.179288242796876
26	16.644792169370454	36.2700484657106	26.23544218287573	20.849717182043214
27	17.041704614470575	36.49379735106269	27.447164235276205	19.017333799190528
28	14.082912361584118	30.14832547771946	37.50412954917744	18.264632611518973
29	14.56815090813081	25.36756291318617	37.351422124234816	22.712864054448207
30	17.632395328812546	32.177447190664395	31.041576697555257	19.148580782967812
31	28.477159694754295	28.745785216927533	23.96502265239424	18.812032435923935
32	30.98559983254513	26.047425460646256	24.534200067341384	18.43277463946723
33	28.434133095964643	28.683201073233487	23.56694732624575	19.315718504556116
34	18.94000220947399	28.083313026963513	27.34609930052705	25.630585463035448
35	19.076482157968933	27.25613459396687	30.300451462137918	23.366931785926283
36	30.630498246956822	25.07382973201406	25.425019808621496	18.870652212407624
37	20.21129548648864	34.59650406241923	27.28541805309398	17.906782397998153
38	19.75740187001844	34.64307216258346	23.491624349249122	22.10790161814898
39	20.705519932045462	33.60615227504463	25.541043622311037	20.14728417059888
40	23.919828866196262	26.913666125222203	24.276199049291886	24.89030595928965
41	17.159261316814785	25.254446302117557	26.080303279377414	31.505989101690247
42	21.724256588646156	25.618480928486516	23.247842194927408	29.40942028793992
43	21.576517837273826	26.368592062913983	25.85428149015806	26.20060860965413
44	19.458911448193913	30.91788844059237	27.47565482096125	22.147545290252467
45	16.195497218864258	39.51734093505151	21.3780880438385	22.909073802245736
46	21.371903630990357	33.829266861643056	25.627308252808223	19.17152125455836
47	20.981439889885735	28.276985579746416	25.96507233912994	24.776502191237903
48	22.03131004364504	24.597154218641723	30.82078787305356	22.550747864659677
49	20.87456054989473	23.56604873634474	32.085632446072715	23.473758267687817
50	19.189334477890196	34.242195350272986	25.55314815685997	21.01532201497685
51	17.82442927648184	34.43248497636973	23.959842545906053	23.783243201242378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1878.0
1	2059.5
2	2241.0
3	4638.5
4	7036.0
5	5784.0
6	4532.0
7	5129.0
8	5726.0
9	6511.0
10	7296.0
11	7732.5
12	8169.0
13	8016.5
14	7864.0
15	7689.5
16	7515.0
17	7445.0
18	7375.0
19	7531.5
20	7688.0
21	7661.5
22	7635.0
23	7906.0
24	8177.0
25	8989.5
26	11469.5
27	13137.0
28	15203.0
29	17269.0
30	19081.5
31	20894.0
32	27243.5
33	33593.0
34	41429.0
35	49265.0
36	52178.0
37	55091.0
38	68153.5
39	81216.0
40	115724.5
41	150233.0
42	186136.5
43	222040.0
44	216891.5
45	211743.0
46	201716.0
47	191689.0
48	175022.0
49	158355.0
50	144246.0
51	130137.0
52	119534.0
53	108931.0
54	97821.0
55	86711.0
56	80693.5
57	74676.0
58	65713.0
59	56750.0
60	52638.0
61	48526.0
62	40620.5
63	32715.0
64	26680.5
65	20646.0
66	16344.5
67	12043.0
68	10661.0
69	9279.0
70	7655.0
71	6031.0
72	5057.5
73	4084.0
74	2924.5
75	1355.5
76	946.0
77	837.0
78	728.0
79	470.5
80	213.0
81	157.5
82	102.0
83	89.0
84	76.0
85	48.0
86	20.0
87	12.5
88	5.0
89	6.0
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1891853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.200335998460453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.2388471214758	14.785743137384216
2	8.038462455565815	2.9260543520461466
3	2.595468585814398	1.4171520100581312
4	1.2420243939183113	0.9042104515038988
5	0.7564128780421098	0.6883484266964444
6	0.49602014222164276	0.5416639950262824
7	0.3522170381661228	0.44873279073042033
8	0.25088775746607644	0.3652993187026278
9	0.19579866490395653	0.32072413403717775
>10	2.2162150851498907	9.632810218581394
>50	0.9660852750253524	12.959171025864805
>100	1.6377215314169702	52.7253092330149
>500	0.012366829255568658	1.428992889898157
>1k	0.0011777932624351102	0.42985006449927315
>5k	2.9444831560877755E-4	0.4259379519561429
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7948	0.42011720783802964	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3543	0.1872767070168771	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08219454682789837	0.0
2	0.0	0.0	0.0	0.23950063773453859	0.0
3	0.0	0.0	0.0	0.3337997191113686	0.0
4	0.0	0.0	0.0	0.5416911356220594	0.0
5	0.0	0.0	0.0	0.8528146742902329	0.0
6	0.0	0.0	0.0	1.1602909951248854	0.0
7	0.0	0.0	0.0	1.3293316129741581	0.0
8	0.0	0.0	0.0	1.7025635712711293	0.0
9	0.0	0.0	0.0	1.832119091705328	0.0
10	0.0	0.0	0.0	2.087477198281262	0.0
11	0.0	0.0	0.0	2.3538826748167008	0.0
12	0.0	0.0	0.0	2.591163266913444	0.0
13	0.0	0.0	0.0	2.6840351760945484	0.0
14	0.0	0.0	0.0	2.7186573163982612	0.0
15	0.0	0.0	0.0	2.7789685562250344	0.0
16	0.0	0.0	0.0	2.910638405838086	0.0
17	5.2858229471317275E-5	0.0	0.0	3.069107377793095	0.0
18	1.0571645894263455E-4	0.0	0.0	3.3181224968324705	0.0
19	1.5857468841395182E-4	0.0	0.0	3.418605991057445	0.0
20	1.5857468841395182E-4	0.0	0.0	3.524269591770608	0.0
21	1.5857468841395182E-4	0.0	0.0	3.653877970434278	0.0
22	1.5857468841395182E-4	0.0	0.0	3.78316919972112	0.0
23	2.114329178852691E-4	0.0	0.0	3.93444945246803	0.0
24	2.114329178852691E-4	0.0	0.0	4.0467203318651075	0.0
25	2.114329178852691E-4	0.0	0.0	4.137530770096831	0.0
26	2.114329178852691E-4	0.0	0.0	4.231565560326304	0.0
27	2.114329178852691E-4	0.0	0.0	4.322640289705384	0.0
28	2.114329178852691E-4	0.0	0.0	4.426665285304937	0.0
29	2.114329178852691E-4	0.0	0.0	4.538936164702014	0.0
30	2.114329178852691E-4	0.0	0.0	4.680966227291444	0.0
31	2.114329178852691E-4	0.0	0.0	4.817710466933741	0.0
32	2.114329178852691E-4	0.0	0.0	4.936112900949492	0.0
33	2.114329178852691E-4	0.0	0.0	5.0576339705040505	0.0
34	2.642911473565864E-4	0.0	0.0	5.1808993616311625	0.0
35	2.642911473565864E-4	0.0	0.0	5.343755566632291	0.0
36	2.642911473565864E-4	0.0	0.0	5.4702453097571535	0.0
37	3.1714937682790365E-4	0.0	0.0	5.601968017599676	0.0
38	3.1714937682790365E-4	0.0	0.0	5.732897852000129	0.0
39	3.1714937682790365E-4	0.0	0.0	5.869113509347714	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGT	30	2.166249E-6	45.000004	15
ATTCGTT	35	1.2124838E-7	45.000004	22
GTTAGTC	25	3.8917642E-5	45.000004	20
CGTTCGA	25	3.8917642E-5	45.000004	43
TCTAGCG	70	0.0	45.000004	1
CGTAAGT	30	2.166249E-6	45.000004	16
CAGTCGA	35	1.2124838E-7	45.000004	42
GCTAACG	60	0.0	45.000004	1
CGCCCAT	25	3.8917642E-5	45.000004	36
ATTTCGC	35	1.2124838E-7	45.000004	16
CGAACTA	55	1.8189894E-12	45.0	40
ACGCATC	20	7.0344453E-4	45.0	19
TCGATTG	20	7.0344453E-4	45.0	1
ACTCGAT	20	7.0344453E-4	45.0	27
TCGTACT	40	6.8193913E-9	45.0	12
ATACCCG	20	7.0344453E-4	45.0	36
GTCGGTA	20	7.0344453E-4	45.0	1
TGCGTAG	160	0.0	45.0	1
TCACGAC	500	0.0	44.55	25
CGACGGT	475	0.0	44.526314	28
>>END_MODULE