FastQCFastQC Report
Thu 26 May 2016
SRR1546296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546296_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2042194
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61000.29869836068463623No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58050.2842531120941497No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29810.14597046118047552No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27070.13255351842185414No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24400.11947934427385448No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATT207.034576E-445.035
CGCTATA207.034576E-445.013
TACGAGT207.034576E-445.021
CTATTCG207.034576E-445.032
ATACGCG551.8189894E-1245.01
CAATACG253.891874E-545.029
GTACGAC207.034576E-445.035
CGTAAGG4050.041.6666682
CGTTTTT54950.041.3967251
TCACGAC3450.040.43478425
TGCGTAG1900.040.2631571
AATCCCG451.929584E-840.033
GCTACGA3900.039.80769310
TGGGCGA8350.039.610786
CGGGATA3650.039.4520576
TAGGGTA9250.039.4054035
CAACGAC403.4599907E-739.37512
ATAGCGC403.4599907E-739.37511
CGTTCCA403.4599907E-739.37529
TACGCGG1600.039.3752