FastQCFastQC Report
Thu 26 May 2016
SRR1546295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546295_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1915622
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51640.26957301597079175No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50850.2654490290882021No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29060.15170007444057335No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21570.11260050260437603No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20790.10852871808738884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA302.1662636E-645.00000434
TCCGCGA406.8193913E-945.00000422
GTCGATC302.1662636E-645.00000436
ACGTTAC502.1827873E-1145.0000041
TAGCGAT207.034464E-445.00000425
CTACGTC253.8917824E-545.0000041
CGCTATA207.034464E-445.00000427
ACGTGCC253.8917824E-545.00000445
ATGCGAT406.8193913E-945.00000412
TACGTCG253.8917824E-545.0000042
TACGTAG650.045.0000041
CGGTCTA3400.043.6764731
GCGATAT2700.043.3333329
TGCGTAG1250.043.21
ACGGGTA1400.041.7857135
GTATGCG650.041.5384641
TAATGCG650.041.5384641
GCGTAAG1850.041.3513531
ACGTTAG1200.041.2500041
TGCGCGT556.184564E-1140.90909237