FastQCFastQC Report
Thu 26 May 2016
SRR1546294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546294_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1719121
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79120.46023520159430314No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33370.19411082756827472No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19270.112092168032384No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18550.10790398116246618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC302.1661326E-645.00000430
TCGATAT302.1661326E-645.00000415
AAGTACG406.8175723E-945.01
GATACGT351.2123928E-745.09
CGTGCTA207.0342637E-445.034
TCCGATT253.8916165E-545.027
TTAGCCG453.8562575E-1045.01
CGGTTGA207.0342637E-445.042
TATATCG406.8175723E-945.01
ATTCGAA207.0342637E-445.014
CGCGACA253.8916165E-545.039
TAGCCGA253.8916165E-545.013
CCGTCGA253.8916165E-545.040
ATCGTGT207.0342637E-445.010
ATGTACG207.0342637E-445.01
TAGCAAC351.2123928E-745.01
CGTAGTC253.8916165E-545.033
CACTACG207.0342637E-445.01
CGCTAAG253.8916165E-545.041
TATTCGA207.0342637E-445.045