##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546294_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1719121
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24158857927976	31.0	31.0	33.0	30.0	34.0
2	31.4823302141036	31.0	31.0	34.0	30.0	34.0
3	31.57711353651081	31.0	31.0	34.0	30.0	34.0
4	35.34953560569617	37.0	35.0	37.0	33.0	37.0
5	35.08724458604136	37.0	35.0	37.0	32.0	37.0
6	35.22626621395469	37.0	35.0	37.0	32.0	37.0
7	35.706135286579595	37.0	35.0	37.0	35.0	37.0
8	35.76375484913511	37.0	35.0	37.0	35.0	37.0
9	37.55626567298055	39.0	37.0	39.0	35.0	39.0
10	36.88533558719834	39.0	37.0	39.0	32.0	39.0
11	36.53793304834273	39.0	35.0	39.0	32.0	39.0
12	35.37878078390061	37.0	35.0	39.0	31.0	39.0
13	34.761580482118475	37.0	34.0	39.0	27.0	39.0
14	35.628008150677005	37.0	34.0	40.0	27.0	41.0
15	35.984277430151806	37.0	34.0	40.0	30.0	41.0
16	36.19445402621456	37.0	35.0	40.0	31.0	41.0
17	36.15861478046048	37.0	34.0	40.0	31.0	41.0
18	36.15958446205939	36.0	35.0	40.0	31.0	41.0
19	36.07549963033434	36.0	35.0	40.0	31.0	41.0
20	35.97780319128206	36.0	34.0	40.0	31.0	41.0
21	35.833597518731956	36.0	34.0	40.0	30.0	41.0
22	35.71215522351248	36.0	34.0	40.0	30.0	41.0
23	35.723807690092784	36.0	34.0	40.0	30.0	41.0
24	35.602862160371494	35.0	34.0	40.0	30.0	41.0
25	35.45830514547841	35.0	34.0	40.0	30.0	41.0
26	35.4177687318112	35.0	34.0	40.0	30.0	41.0
27	35.32402605750264	35.0	34.0	40.0	29.0	41.0
28	35.33258799118852	36.0	34.0	40.0	29.0	41.0
29	35.40875656803681	36.0	34.0	40.0	30.0	41.0
30	35.34299156371192	36.0	34.0	40.0	29.0	41.0
31	35.19697740880368	35.0	34.0	40.0	29.0	41.0
32	35.018529818436285	35.0	34.0	40.0	29.0	41.0
33	34.868140171634224	35.0	34.0	40.0	28.0	41.0
34	34.777306542122396	35.0	34.0	40.0	27.0	41.0
35	34.635037324307014	35.0	33.0	40.0	27.0	41.0
36	34.432507077744965	35.0	33.0	40.0	25.0	41.0
37	34.334090503228104	35.0	33.0	40.0	25.0	41.0
38	34.378594642261945	35.0	33.0	40.0	26.0	41.0
39	34.367184159811906	35.0	33.0	40.0	26.0	41.0
40	34.15158793360095	35.0	33.0	40.0	24.0	41.0
41	34.123787098174006	35.0	33.0	40.0	24.0	41.0
42	34.09706239409559	35.0	33.0	39.0	24.0	41.0
43	34.070351068947446	35.0	33.0	39.0	25.0	41.0
44	33.84028349371568	35.0	33.0	39.0	23.0	41.0
45	33.84910311723259	35.0	33.0	39.0	24.0	41.0
46	33.814821644317064	35.0	33.0	39.0	24.0	41.0
47	33.788074835919055	35.0	33.0	39.0	24.0	41.0
48	33.705060900308936	35.0	33.0	39.0	23.0	41.0
49	33.68816621982978	35.0	33.0	39.0	24.0	41.0
50	33.597854950291456	35.0	33.0	39.0	24.0	40.0
51	33.50872102661767	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	3.0
14	14.0
15	55.0
16	178.0
17	478.0
18	1029.0
19	1937.0
20	3378.0
21	5373.0
22	8214.0
23	11720.0
24	16394.0
25	22296.0
26	28356.0
27	34199.0
28	38771.0
29	44959.0
30	53907.0
31	66682.0
32	83598.0
33	109343.0
34	195923.0
35	282832.0
36	111992.0
37	134329.0
38	186595.0
39	276424.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.20708664486095	23.991970315062176	29.990908144336554	12.810034895740325
2	29.080501023488164	27.477297991240874	30.728436218276666	12.713764766994295
3	29.116914981551616	26.699284110891554	30.7172095506948	13.466591356862024
4	26.20368199795128	28.327499925834193	30.455680548373266	15.013137527841264
5	22.313787103990933	33.4277226559387	29.738162700589427	14.520327539480931
6	22.036843247217618	40.798466192897415	26.78357137164865	10.381119188236314
7	87.03389697409317	5.378562649167802	5.5306752695127335	2.0568651072263093
8	89.24304920945065	4.041425821684454	4.9617798863488956	1.7537450825160068
9	86.18433490138274	5.154843667199692	6.119115524736188	2.5417059066813796
10	57.087779161559894	21.11241733420742	11.536767918023221	10.263035586209464
11	53.824483558748916	18.97207933589317	15.490125476915237	11.713311628442675
12	48.30148663183104	20.536599808855804	18.81368443524336	12.348229124069801
13	23.746961383171982	42.724799476011285	19.217030098521278	14.311209042295452
14	15.888584922178254	46.25357959096538	23.916873797714064	13.9409616891423
15	14.938913549424388	23.654181410150883	49.01167515259252	12.395229887832212
16	15.572144136451127	17.052086502346256	48.68208811363482	18.6936812475678
17	15.371809197840058	17.75831951328615	28.69024344417874	38.179627844695055
18	21.93685028569833	23.648015468370172	32.736671822402265	21.678462423529236
19	31.43676332265152	24.412301402868096	23.623584378295654	20.527350896184736
20	34.910864331248355	22.653262917502605	22.34327891986661	20.092593831382434
21	24.077479130322995	28.338959270464382	25.021857100227386	22.56170449898524
22	22.847431914332965	26.63872990906399	23.5558171879699	26.958020988633148
23	21.76589082443877	31.22566707055524	22.14352567387636	24.86491643112963
24	21.701846466886273	24.0924867999402	36.827134332022005	17.37853240115152
25	18.812579219263796	24.101328527776694	35.21171575473745	21.874376498222055
26	17.83923295684248	35.43665629120928	26.03464212234043	20.689468629607806
27	19.063230569575964	35.00736713704271	27.05464013295166	18.87476216042966
28	15.43975089595206	30.731693696953265	36.83649958321723	16.992055823877436
29	16.76426499356357	25.445445666709904	36.86069799624343	20.929591343483096
30	19.406196538812566	30.52792677187935	30.69295296840653	19.372923720901554
31	30.17216356498466	27.42418945495983	22.63069324381472	19.77295373624079
32	32.34647241235492	27.376432490790354	24.194922870466943	16.082172226387787
33	29.844961465772336	28.536385745971344	23.075862606529732	18.54279018172659
34	21.04598803690956	28.026415825296763	26.98530237254969	23.94229376524398
35	20.873283497787533	25.771019026583936	29.6046642441108	23.751033231517734
36	30.69493072331732	25.350397092467603	24.786213419532423	19.16845876468265
37	22.334146345719702	33.11756415051646	27.25392802484525	17.29436147891859
38	21.027024857470767	34.49460509178819	21.245683113637725	23.232686937103324
39	22.070988604059867	32.45356202384823	24.405728276252805	21.069721095839096
40	24.54649789049171	26.332236067152927	23.874526574918228	25.24673946743714
41	16.850297332183136	25.83832086281303	26.02801082646306	31.283370978540777
42	22.477533576752307	26.814808265386787	21.942550873382384	28.765107284478518
43	23.16177860662513	27.308025438581694	23.341870642031594	26.188325312761577
44	20.569756288242655	31.636691076428015	25.755953187704645	22.037599447624686
45	18.007051277949603	37.7541778618259	21.19327260850167	23.04549825172283
46	21.61674483646003	34.110048100162814	23.87510826753905	20.398098795838106
47	23.109833455585733	28.694373461786576	24.709546332108097	23.486246750519594
48	24.449413392076533	23.486246750519594	29.03256955153244	23.03177030587143
49	21.353063571441453	23.67576220638338	30.259650135156278	24.711524087018887
50	18.81921051514117	33.693963368488895	25.356330357199987	22.13049575916995
51	18.260262075793385	35.29530498434956	21.71016467136403	24.734268268493025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	975.0
1	1537.5
2	2100.0
3	4599.0
4	7098.0
5	5750.0
6	4402.0
7	4809.0
8	5216.0
9	5724.0
10	6232.0
11	6289.5
12	6347.0
13	6355.5
14	6364.0
15	5978.0
16	5592.0
17	5467.5
18	5343.0
19	5077.0
20	4811.0
21	4791.0
22	4771.0
23	5531.0
24	6291.0
25	6457.0
26	8277.5
27	9932.0
28	13082.5
29	16233.0
30	21065.0
31	25897.0
32	26345.5
33	26794.0
34	31855.0
35	36916.0
36	38384.5
37	39853.0
38	52291.0
39	64729.0
40	92741.5
41	120754.0
42	149334.0
43	177914.0
44	180636.5
45	183359.0
46	169555.0
47	155751.0
48	144929.5
49	134108.0
50	126434.0
51	118760.0
52	112213.5
53	105667.0
54	94904.0
55	84141.0
56	80685.0
57	77229.0
58	75952.5
59	74676.0
60	71197.0
61	67718.0
62	58381.5
63	49045.0
64	39542.5
65	30040.0
66	23007.5
67	15975.0
68	13668.0
69	11361.0
70	9619.0
71	7877.0
72	6754.0
73	5631.0
74	4434.0
75	2499.5
76	1762.0
77	1252.5
78	743.0
79	548.5
80	354.0
81	345.5
82	337.0
83	213.0
84	89.0
85	68.0
86	47.0
87	30.5
88	14.0
89	13.5
90	13.0
91	6.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1719121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.273160243785963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66692547432073	13.289789648609474
2	7.449125580364428	2.424416284907109
3	2.3481233525440435	1.146341627643754
4	1.0309274053000173	0.6710578746463063
5	0.6040077848245993	0.4914557735472447
6	0.4025448189005537	0.3930405805964707
7	0.28221168707908273	0.3214733204554966
8	0.1978804954524556	0.2576112809294055
9	0.17201727993135332	0.25193382849207896
>10	2.503146311171514	10.271930091260641
>50	1.3929540907413092	16.533555413576632
>100	1.9407165239949307	52.164216410010155
>500	0.0076078116490374366	0.8362017668454023
>1k	0.0014491069807690356	0.48053303733480496
>5k	3.622767451922589E-4	0.46644306114501005
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7912	0.46023520159430314	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3337	0.19411082756827472	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1927	0.112092168032384	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1855	0.10790398116246618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035832265442630275	0.0
2	0.0	0.0	0.0	0.11343006106027441	0.0
3	0.0	0.0	0.0	0.16252491825764445	0.0
4	0.0	0.0	0.0	0.2532107978437818	0.0
5	0.0	0.0	0.0	0.40130973910504264	0.0
6	0.0	0.0	0.0	0.5509210811804405	0.0
7	0.0	0.0	0.0	0.6303802931847148	0.0
8	0.0	0.0	0.0	0.8285047998366607	0.0
9	0.0	0.0	0.0	0.8990641147423596	0.0
10	0.0	0.0	0.0	1.0263384601781957	0.0
11	0.0	0.0	0.0	1.1856058997592374	0.0
12	0.0	0.0	0.0	1.3271898836672928	0.0
13	0.0	0.0	0.0	1.3807056047829094	0.0
14	0.0	0.0	0.0	1.4047295100228547	0.0
15	0.0	0.0	0.0	1.4431212229971013	0.0
16	0.0	0.0	0.0	1.5321783632449373	0.0
17	0.0	0.0	0.0	1.6338000641025268	0.0
18	0.0	0.0	0.0	1.7763729254659795	0.0
19	0.0	0.0	0.0	1.8418715145705276	0.0
20	0.0	0.0	0.0	1.9012041618943634	0.0
21	0.0	0.0	0.0	1.984095360361487	0.0
22	0.0	0.0	0.0	2.0769335026446654	0.0
23	0.0	0.0	0.0	2.1762866022810496	0.0
24	5.8169262082192006E-5	0.0	0.0	2.252779181919132	0.0
25	5.8169262082192006E-5	0.0	0.0	2.315776492754146	0.0
26	5.8169262082192006E-5	0.0	0.0	2.3746437859813243	0.0
27	5.8169262082192006E-5	0.0	0.0	2.4350234800226396	0.0
28	5.8169262082192006E-5	0.0	0.0	2.5022089777275713	0.0
29	1.1633852416438401E-4	0.0	0.0	2.5716630766537083	0.0
30	1.1633852416438401E-4	0.0	0.0	2.664733895985216	0.0
31	1.1633852416438401E-4	0.0	0.0	2.7625164255453805	0.0
32	1.1633852416438401E-4	0.0	0.0	2.843022684267134	0.0
33	1.1633852416438401E-4	0.0	0.0	2.9238197892992988	0.0
34	1.1633852416438401E-4	0.0	0.0	3.006478310718094	0.0
35	1.1633852416438401E-4	0.0	0.0	3.12235148078582	0.0
36	1.1633852416438401E-4	0.0	0.0	3.222577119353437	0.0
37	1.1633852416438401E-4	0.0	0.0	3.325304036190588	0.0
38	1.1633852416438401E-4	0.0	0.0	3.4216323341986983	0.0
39	1.1633852416438401E-4	0.0	0.0	3.515168507626863	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATC	30	2.1661326E-6	45.000004	30
TCGATAT	30	2.1661326E-6	45.000004	15
AAGTACG	40	6.8175723E-9	45.0	1
GATACGT	35	1.2123928E-7	45.0	9
CGTGCTA	20	7.0342637E-4	45.0	34
TCCGATT	25	3.8916165E-5	45.0	27
TTAGCCG	45	3.8562575E-10	45.0	1
CGGTTGA	20	7.0342637E-4	45.0	42
TATATCG	40	6.8175723E-9	45.0	1
ATTCGAA	20	7.0342637E-4	45.0	14
CGCGACA	25	3.8916165E-5	45.0	39
TAGCCGA	25	3.8916165E-5	45.0	13
CCGTCGA	25	3.8916165E-5	45.0	40
ATCGTGT	20	7.0342637E-4	45.0	10
ATGTACG	20	7.0342637E-4	45.0	1
TAGCAAC	35	1.2123928E-7	45.0	1
CGTAGTC	25	3.8916165E-5	45.0	33
CACTACG	20	7.0342637E-4	45.0	1
CGCTAAG	25	3.8916165E-5	45.0	41
TATTCGA	20	7.0342637E-4	45.0	45
>>END_MODULE