FastQCFastQC Report
Thu 26 May 2016
SRR1546293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546293_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2477892
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC64210.25913155214190126No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48970.1976276609311463No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT25260.10194148897530644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA650.045.00000410
CGTCTAG302.1665219E-645.00000412
CGATTAC302.1665219E-645.00000410
CCGTATT253.892115E-545.00000418
CGAGGTT207.034866E-445.00000411
CGGTGAA207.034866E-445.00000442
ACTCGCG253.892115E-545.00000437
CACCGTA207.034866E-445.00000418
CCGTTCG207.034866E-445.00000438
ATCGAAT207.034866E-445.00000438
GTACGAC207.034866E-445.00000438
TAATCCG253.892115E-545.0000041
GCTCGAT453.8562575E-1045.08
ACGACCG453.8562575E-1045.01
CTATGCG900.042.51
CGTTTTT59600.041.791111
CTCACGT1400.041.78571345
TATTGCG1300.041.5384641
TCGGTGT556.184564E-1140.90909232
TATCCGT556.184564E-1140.90909242