##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546293_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2477892 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24203032254836 31.0 31.0 33.0 30.0 34.0 2 31.473931067213584 31.0 31.0 34.0 30.0 34.0 3 31.556332156526597 31.0 31.0 34.0 30.0 34.0 4 35.33325463740954 37.0 35.0 37.0 33.0 37.0 5 35.0567684144426 37.0 35.0 37.0 32.0 37.0 6 35.19383451740431 37.0 35.0 37.0 32.0 37.0 7 35.755534543071285 37.0 35.0 37.0 35.0 37.0 8 35.83161978003884 37.0 35.0 37.0 35.0 37.0 9 37.58443830481716 39.0 37.0 39.0 35.0 39.0 10 36.808814508461225 39.0 37.0 39.0 32.0 39.0 11 36.480729587891645 39.0 35.0 39.0 32.0 39.0 12 35.7082290107882 37.0 35.0 39.0 31.0 39.0 13 35.25165019298662 37.0 35.0 39.0 30.0 39.0 14 36.20450407039532 38.0 35.0 40.0 30.0 41.0 15 36.485309287087574 38.0 35.0 40.0 31.0 41.0 16 36.62325880223997 38.0 35.0 40.0 31.0 41.0 17 36.57211613742649 38.0 35.0 40.0 31.0 41.0 18 36.516030965029955 38.0 35.0 40.0 31.0 41.0 19 36.47943816760375 38.0 35.0 40.0 31.0 41.0 20 36.43053571342092 38.0 35.0 40.0 31.0 41.0 21 36.2832726365798 38.0 35.0 40.0 31.0 41.0 22 36.18658722817621 38.0 35.0 40.0 30.0 41.0 23 36.16700364664804 38.0 34.0 40.0 30.0 41.0 24 36.101876917960915 37.0 34.0 40.0 30.0 41.0 25 35.94314320398145 37.0 34.0 40.0 30.0 41.0 26 35.912236691510365 37.0 34.0 40.0 30.0 41.0 27 35.851253807672 37.0 34.0 40.0 30.0 41.0 28 35.86466157524218 37.0 34.0 40.0 30.0 41.0 29 35.909398391858886 37.0 34.0 40.0 30.0 41.0 30 35.79854650646598 37.0 34.0 40.0 30.0 41.0 31 35.68737903024022 37.0 34.0 40.0 29.0 41.0 32 35.54152037296218 37.0 34.0 40.0 29.0 41.0 33 35.414830832013664 37.0 34.0 40.0 29.0 41.0 34 35.281363755966765 37.0 34.0 40.0 27.0 41.0 35 35.14974018238083 37.0 34.0 40.0 27.0 41.0 36 35.02673239995932 37.0 34.0 40.0 27.0 41.0 37 34.99004960668181 37.0 34.0 40.0 27.0 41.0 38 34.97917907640849 37.0 34.0 40.0 27.0 41.0 39 34.937661931997035 37.0 34.0 40.0 27.0 41.0 40 34.781051393684635 36.0 34.0 40.0 26.0 41.0 41 34.72391331018463 36.0 34.0 40.0 25.0 41.0 42 34.69803566902835 36.0 33.0 40.0 26.0 41.0 43 34.70041268949575 36.0 33.0 40.0 26.0 41.0 44 34.461113720856275 36.0 33.0 40.0 25.0 41.0 45 34.44427965383479 36.0 33.0 40.0 25.0 41.0 46 34.40285492668769 36.0 33.0 40.0 25.0 41.0 47 34.36623387944269 35.0 33.0 40.0 25.0 41.0 48 34.290126849757776 35.0 33.0 40.0 24.0 41.0 49 34.23843250634007 36.0 33.0 40.0 24.0 41.0 50 34.18503873453726 35.0 33.0 40.0 24.0 41.0 51 34.10963754675345 35.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 2.0 11 2.0 12 5.0 13 14.0 14 27.0 15 81.0 16 249.0 17 676.0 18 1373.0 19 2629.0 20 4676.0 21 7364.0 22 10844.0 23 15388.0 24 21288.0 25 29583.0 26 37734.0 27 44253.0 28 50914.0 29 59716.0 30 72054.0 31 89483.0 32 111980.0 33 143504.0 34 236536.0 35 324886.0 36 175150.0 37 218940.0 38 319385.0 39 498973.0 40 180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.33930938071554 23.68254952193235 29.194533095066287 12.783608002285815 2 30.71235550217685 25.524599135071263 30.256080571711763 13.506964791040128 3 29.247279542449796 25.714881843115034 30.990414432913138 14.047424181522036 4 25.983456906112128 28.72982357584592 30.361250611406792 14.925468906635157 5 23.498239632720068 32.48604055382559 29.415890603787414 14.599829209666925 6 22.30710620156165 40.53320322273933 26.99379149696597 10.165899078733052 7 88.06953652540143 4.644068425903954 5.567595359281195 1.7187996894134208 8 89.76706813694867 3.4157663045847033 5.1515158852766785 1.6656496731899533 9 85.55752228103566 5.077662787562977 6.610054029796294 2.7547609016050743 10 47.656435389435856 29.64031523569227 12.227288356393256 10.47596101847861 11 41.977979669816115 22.16767316735354 22.86338549057021 12.990961672260132 12 39.42395390920993 23.05734067505767 23.960648809552637 13.558056606179768 13 22.788886682712565 37.27470769508921 24.14625011905281 15.790155503145414 14 16.521260813627066 40.24348115252804 26.98959438103033 16.24566365281457 15 15.874138178742253 25.17409959756115 43.99663100732397 14.955131216372628 16 17.965472264327907 21.595008983442376 42.828743141347566 17.610775610882154 17 18.7207109914395 20.942478526102025 28.689385978081365 31.64742450437711 18 22.17061921988529 24.555993562269865 32.706792709286766 20.56659450855808 19 28.231416058488424 25.8524584606593 25.624320995426757 20.291804485425516 20 31.78766467626515 24.130874146250118 25.682999904757754 18.39846127272698 21 23.354205913736354 28.480377675863195 28.307367714169946 19.858048696230508 22 23.53730509642874 25.553454307128803 26.871308354036415 24.037932242406047 23 20.3154536194475 31.0917102117445 26.403652782284297 22.189183386523705 24 20.62604019868501 24.28988026919656 37.100204528688096 17.983875003430335 25 19.01430732251446 26.83325181242766 33.88525407887026 20.26718678618761 26 18.54935566199011 33.87092738505149 27.088831958777863 20.490884994180536 27 18.424047537180797 33.77536228374764 28.977130560976832 18.823459618094734 28 16.47464861261104 29.91006064832527 35.67411331890171 17.941177420161978 29 18.022335113879056 25.773843250634005 35.203511694617845 21.000309940869094 30 19.752878656535476 30.322306218350114 30.538538402803674 19.38627672231074 31 26.731592821640334 28.341267496727056 25.667422147535085 19.259717534097533 32 28.705004092188034 27.258088730259427 26.697410540895245 17.339496636657287 33 27.93289618756588 28.17527963284921 24.911537710279543 18.98028646930536 34 20.457711635535368 27.831721479386513 29.147073399486338 22.563493485591785 35 20.200436500057307 27.314588367854615 31.123148224377818 21.361826907710263 36 26.844269241758724 27.73502638533076 26.578155948685417 18.842548424225107 37 20.803610488269868 32.33910113919412 28.4861487102747 18.37113966226131 38 20.506059182563245 32.620146479346154 25.563139959287973 21.310654378802628 39 21.774112834619103 31.358751713149722 26.871389067804408 19.995746384426763 40 24.165500352719167 26.888338959082965 26.44150753947307 22.504653148724802 41 18.41851864407327 26.06271782628137 28.091296957252375 27.427466572392984 42 21.263194683222675 27.04274439725379 25.390654637086683 26.303406282436846 43 22.252382266862316 27.78414071315457 26.157395076137295 23.806081943845818 44 20.706108256534183 30.423198428341507 27.80581235986072 21.064880955263586 45 18.831530994894045 35.26352238112073 23.197257991873737 22.707688632111488 46 21.268844646982192 32.99397229580627 25.97058306011723 19.766599997094307 47 21.283494195872944 28.703188032408196 27.46883237848946 22.544485393229408 48 22.12981841016477 25.735181355765302 30.591567348375154 21.543432885694777 49 21.64715007756593 25.988622587263688 30.857478857028475 21.506748478141905 50 18.838351308289468 32.894169721682786 27.06792709286765 21.199551877160104 51 18.248051166071807 33.18885568862566 25.481134771006968 23.08195837429557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1157.0 1 1852.5 2 2548.0 3 6708.0 4 10868.0 5 8565.5 6 6263.0 7 6712.0 8 7161.0 9 7607.5 10 8054.0 11 8111.0 12 8168.0 13 8088.5 14 8009.0 15 7809.5 16 7610.0 17 7466.0 18 7322.0 19 7868.0 20 8414.0 21 8975.5 22 9537.0 23 10133.5 24 10730.0 25 14340.5 26 20974.0 27 23997.0 28 30844.5 29 37692.0 30 42053.0 31 46414.0 32 56090.0 33 65766.0 34 77600.0 35 89434.0 36 94459.5 37 99485.0 38 109565.0 39 119645.0 40 149258.5 41 178872.0 42 211986.0 43 245100.0 44 240878.5 45 236657.0 46 222636.5 47 208616.0 48 201652.5 49 194689.0 50 179901.0 51 165113.0 52 154056.0 53 142999.0 54 132797.5 55 122596.0 56 110791.0 57 98986.0 58 92587.0 59 86188.0 60 80159.0 61 74130.0 62 61932.5 63 49735.0 64 41129.0 65 32523.0 66 25634.0 67 18745.0 68 14216.0 69 9687.0 70 8531.0 71 7375.0 72 5730.5 73 4086.0 74 3325.0 75 2093.5 76 1623.0 77 1111.0 78 599.0 79 433.5 80 268.0 81 338.5 82 409.0 83 241.0 84 73.0 85 42.5 86 12.0 87 11.5 88 11.0 89 7.0 90 3.0 91 3.5 92 4.0 93 3.5 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2477892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.227680723049396 #Duplication Level Percentage of deduplicated Percentage of total 1 81.49701723888231 13.225075756334348 2 7.5066549564220955 2.436311998618281 3 2.4671307294132623 1.2010742933682717 4 1.15914354807423 0.7524084564132507 5 0.6453185537123955 0.5236011727152379 6 0.4229149475552318 0.41177572451588884 7 0.32256379714856503 0.3664123619058969 8 0.22876204392240818 0.2969821928260034 9 0.1887498843438551 0.27566755736801224 >10 2.3470000231172983 9.396709679032051 >50 1.240726472686885 14.732122081706125 >100 1.968981402618206 55.38312881373074 >500 0.004280941787767882 0.43300090596940344 >1k 5.036402103256331E-4 0.30333764864202356 >5k 2.5182010516281654E-4 0.26239135685442994 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 6421 0.25913155214190126 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4897 0.1976276609311463 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2526 0.10194148897530644 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.025788048873800797 0.0 2 0.0 0.0 0.0 0.0836598205248655 0.0 3 0.0 0.0 0.0 0.12002137300576458 0.0 4 0.0 0.0 0.0 0.19585195803529776 0.0 5 0.0 0.0 0.0 0.332258225943665 0.0 6 0.0 0.0 0.0 0.4670905753761665 0.0 7 0.0 0.0 0.0 0.5568442853845124 0.0 8 0.0 0.0 0.0 0.7796546419295111 0.0 9 0.0 0.0 0.0 0.8573012867388894 0.0 10 0.0 0.0 0.0 0.9937882684152497 0.0 11 0.0 0.0 0.0 1.1506151196258756 0.0 12 0.0 0.0 0.0 1.29432598353762 0.0 13 1.2107065198967509E-4 0.0 0.0 1.346668862081156 0.0 14 1.2107065198967509E-4 0.0 0.0 1.3666051627754559 0.0 15 1.6142753598623346E-4 0.0 0.0 1.4009085141725306 0.0 16 1.6142753598623346E-4 0.0 0.0 1.477425166230005 0.0 17 1.6142753598623346E-4 0.0 0.0 1.5698020736981273 0.0 18 1.6142753598623346E-4 0.0 0.0 1.6949084140874582 0.0 19 1.6142753598623346E-4 0.0 0.0 1.7560490933422441 0.0 20 1.6142753598623346E-4 0.0 0.0 1.824332941064421 0.0 21 1.6142753598623346E-4 0.0 0.0 1.91287594455287 0.0 22 1.6142753598623346E-4 0.0 0.0 2.0106203175925343 0.0 23 1.6142753598623346E-4 0.0 0.0 2.116395710547514 0.0 24 1.6142753598623346E-4 0.0 0.0 2.1943652104288645 0.0 25 1.6142753598623346E-4 0.0 0.0 2.2649494005388453 0.0 26 1.6142753598623346E-4 0.0 0.0 2.332870036305053 0.0 27 2.0178441998279183E-4 0.0 0.0 2.403575297067023 0.0 28 2.0178441998279183E-4 0.0 0.0 2.478518030648632 0.0 29 2.4214130397935018E-4 0.0 0.0 2.5691192352209056 0.0 30 2.4214130397935018E-4 0.0 0.0 2.675984264043792 0.0 31 2.4214130397935018E-4 0.0 0.0 2.7799032403349297 0.0 32 3.228550719724669E-4 0.0 0.0 2.87417692135089 0.0 33 4.0356883996558367E-4 0.0 0.0 2.9661502599790466 0.0 34 4.0356883996558367E-4 0.0 0.0 3.0565496801313374 0.0 35 4.43925723962142E-4 0.0 0.0 3.179315321248868 0.0 36 4.8428260795870036E-4 0.0 0.0 3.282467516744071 0.0 37 5.246394919552588E-4 0.0 0.0 3.3875971995551057 0.0 38 5.246394919552588E-4 0.0 0.0 3.503260029089242 0.0 39 5.246394919552588E-4 0.0 0.0 3.630949210054353 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 65 0.0 45.000004 10 CGTCTAG 30 2.1665219E-6 45.000004 12 CGATTAC 30 2.1665219E-6 45.000004 10 CCGTATT 25 3.892115E-5 45.000004 18 CGAGGTT 20 7.034866E-4 45.000004 11 CGGTGAA 20 7.034866E-4 45.000004 42 ACTCGCG 25 3.892115E-5 45.000004 37 CACCGTA 20 7.034866E-4 45.000004 18 CCGTTCG 20 7.034866E-4 45.000004 38 ATCGAAT 20 7.034866E-4 45.000004 38 GTACGAC 20 7.034866E-4 45.000004 38 TAATCCG 25 3.892115E-5 45.000004 1 GCTCGAT 45 3.8562575E-10 45.0 8 ACGACCG 45 3.8562575E-10 45.0 1 CTATGCG 90 0.0 42.5 1 CGTTTTT 5960 0.0 41.79111 1 CTCACGT 140 0.0 41.785713 45 TATTGCG 130 0.0 41.538464 1 TCGGTGT 55 6.184564E-11 40.909092 32 TATCCGT 55 6.184564E-11 40.909092 42 >>END_MODULE