FastQCFastQC Report
Thu 26 May 2016
SRR1546292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546292_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1582554
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44820.28321308467199224No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.2620447706681731No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22240.14053232938654858No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20060.12675712803480954No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19430.12277622122215102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG207.0340926E-445.00000441
GCACGAT207.0340926E-445.00000440
TATGCGA207.0340926E-445.00000441
TACGACT351.21232E-745.00000413
CGTAAAC207.0340926E-445.00000440
ACGTATG207.0340926E-445.00000411
ACGTAAG302.1660217E-645.0000041
ACGTAAC207.0340926E-445.00000419
ATACGCG302.1660217E-645.0000041
ATCGCAC207.0340926E-445.00000427
TACACGA253.8914754E-545.045
CCGCACA253.8914754E-545.042
CGGACGT253.8914754E-545.036
CGACGGT1850.043.78378328
CGGTCTA1850.043.78378331
TTACGAG1000.042.751
GCTACGA2450.041.3265310
ATACCGG603.6379788E-1241.2500042
CGCACGG556.184564E-1140.9090922
CGTTTTT36700.040.524521