##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546292_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1582554
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18728776395624	31.0	31.0	33.0	30.0	34.0
2	31.427423013685473	31.0	31.0	34.0	30.0	34.0
3	31.521965127256323	31.0	31.0	34.0	30.0	34.0
4	35.30240295117892	37.0	35.0	37.0	33.0	37.0
5	35.006639899807524	37.0	35.0	37.0	32.0	37.0
6	35.15738736245335	37.0	35.0	37.0	32.0	37.0
7	35.677846064020564	37.0	35.0	37.0	35.0	37.0
8	35.74032039349052	37.0	35.0	37.0	35.0	37.0
9	37.44813826258061	39.0	37.0	39.0	35.0	39.0
10	36.74072985819125	39.0	37.0	39.0	32.0	39.0
11	36.46933311596318	39.0	35.0	39.0	32.0	39.0
12	35.640159514304095	37.0	35.0	39.0	31.0	39.0
13	35.19005670580593	37.0	35.0	39.0	30.0	39.0
14	36.155565623669084	38.0	35.0	40.0	30.0	41.0
15	36.41166999672681	38.0	35.0	40.0	31.0	41.0
16	36.565155438613786	38.0	35.0	40.0	31.0	41.0
17	36.47735938236547	38.0	35.0	40.0	31.0	41.0
18	36.46169040677285	38.0	35.0	40.0	31.0	41.0
19	36.40304469863272	38.0	35.0	40.0	31.0	41.0
20	36.299751540863696	38.0	35.0	40.0	31.0	41.0
21	36.171227648472026	38.0	34.0	40.0	30.0	41.0
22	36.05632098493953	37.0	34.0	40.0	30.0	41.0
23	35.992996131569605	37.0	34.0	40.0	30.0	41.0
24	35.91063875229534	37.0	34.0	40.0	30.0	41.0
25	35.7917638197496	37.0	34.0	40.0	30.0	41.0
26	35.72473672304389	37.0	34.0	40.0	30.0	41.0
27	35.64739718202349	37.0	34.0	40.0	29.0	41.0
28	35.624424822154566	37.0	34.0	40.0	29.0	41.0
29	35.65958254821005	37.0	34.0	40.0	29.0	41.0
30	35.58365464938321	37.0	34.0	40.0	29.0	41.0
31	35.496240254676934	37.0	34.0	40.0	29.0	41.0
32	35.30856008704916	37.0	34.0	40.0	29.0	41.0
33	35.15378495773288	37.0	34.0	40.0	27.0	41.0
34	35.01906538418278	36.0	34.0	40.0	27.0	41.0
35	34.87355881695032	36.0	34.0	40.0	26.0	41.0
36	34.715798639414515	36.0	33.0	40.0	25.0	41.0
37	34.62044138778203	36.0	33.0	40.0	25.0	41.0
38	34.63022936342141	36.0	33.0	40.0	25.0	41.0
39	34.5221313143185	36.0	33.0	40.0	25.0	41.0
40	34.390647649306125	36.0	33.0	40.0	24.0	41.0
41	34.339326809701284	36.0	33.0	40.0	24.0	41.0
42	34.279025549839055	36.0	33.0	40.0	24.0	41.0
43	34.25382640971493	35.0	33.0	40.0	24.0	41.0
44	34.04814812006415	35.0	33.0	40.0	23.0	41.0
45	34.058318389135536	35.0	33.0	40.0	23.0	41.0
46	33.98584187332628	35.0	33.0	40.0	23.0	41.0
47	33.923236742632476	35.0	33.0	40.0	23.0	41.0
48	33.85185655592163	35.0	33.0	39.0	23.0	41.0
49	33.75809735402394	35.0	33.0	39.0	24.0	41.0
50	33.69512635903735	35.0	33.0	39.0	24.0	41.0
51	33.61511076399289	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	9.0
14	15.0
15	56.0
16	171.0
17	421.0
18	981.0
19	1908.0
20	3240.0
21	5267.0
22	7973.0
23	11628.0
24	15861.0
25	20842.0
26	26177.0
27	30559.0
28	35017.0
29	40185.0
30	48044.0
31	59289.0
32	73168.0
33	93838.0
34	154900.0
35	212494.0
36	115632.0
37	142658.0
38	196868.0
39	285178.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.41431256058245	24.53988931815281	29.7654929942359	13.280305127028841
2	29.414730871742766	27.433060736000165	29.966497193776643	13.185711198480432
3	29.16197488363746	26.057309892742996	31.273940731248352	13.506774492371193
4	26.929128484715214	27.21101460044965	30.715981887505894	15.14387502732924
5	23.618467363514924	32.816510526655016	29.47330707198617	14.09171503784389
6	22.214976550563204	41.848998517586125	25.90439251993929	10.031632411911378
7	85.56125099048752	5.572321702766541	6.858470548240376	2.007956758505555
8	87.16012218224465	5.123553445885575	5.750388296386727	1.9659360754830484
9	82.32211981392105	6.528750361757007	7.4037915925775675	3.745338231744383
10	49.303088551796655	25.155286960192196	13.59694519112776	11.944679296883393
11	44.497944461926735	22.08240603480197	19.431122097571393	13.9885274056999
12	39.33369730195621	21.86364572709683	22.92086083634429	15.881796134602673
13	23.348081645239276	37.25136709395066	23.51452146340662	15.886029797403436
14	16.812759627791532	40.236225746483214	26.69444454975944	16.25657007596581
15	16.922139781644102	23.955391095659294	42.77225295313777	16.350216169558827
16	18.51260683679672	19.302090165643637	41.536971250270135	20.648331747289507
17	17.9717722112484	21.253303204819552	27.9758542204563	32.79907036347575
18	22.06269106772976	23.79369045226893	32.511370860014885	21.632247619986426
19	29.146746335354116	24.688762595146834	24.122146858811767	22.042344210687283
20	30.72874606490521	24.00398343437254	24.946826459002345	20.320444041719902
21	23.457335421097795	28.21186512434963	26.886475911722446	21.444323542830134
22	23.591675228775763	24.842943747891066	25.562666424020918	26.00271459931225
23	21.62390667237895	29.639304567174328	24.673976369842674	24.062812390604048
24	21.362746547669147	24.899940223208812	35.17011109889457	18.567202130227468
25	19.71957986899657	26.512017915344437	31.987850019651777	21.780552196007214
26	18.130882105760687	32.738788060312636	26.790491825239453	22.339838008687224
27	20.62798489024703	31.715379064474263	27.4850020915558	20.171633953722907
28	17.20491054333691	29.84631172143257	34.208690509139025	18.740087226091497
29	17.95730193092937	25.668950317019195	34.42542876893932	21.94831898311211
30	19.716799553127412	30.21950593787005	29.490178534192196	20.573515974810338
31	28.115185958899346	26.838262706991355	24.35878965267536	20.687761681433937
32	29.202036707752153	26.78524713848627	25.582444580090158	18.43027157367142
33	27.695611018644545	27.529234389474226	24.454773739158348	20.320380852722877
34	20.52618741603762	28.19827948998897	27.137020284931822	24.138512809041586
35	21.990213288140563	25.55388315343426	29.010384479771307	23.445519078653874
36	28.123147772524664	26.78303552359035	25.118384585928823	19.975432117956167
37	21.562613345263415	31.598226663987454	27.49119461326438	19.347965377484748
38	21.672246255104092	31.587990046469187	23.799882973977507	22.939880724449214
39	21.813220907469823	30.42297450829482	26.553406708396682	21.210397875838677
40	22.976909476706638	26.53982107403602	25.81959288592996	24.663676563327382
41	18.660469089838326	25.310226380900748	27.070292704072024	28.959011825188902
42	21.350235126257935	27.304850261033746	24.5216276980122	26.823286914696116
43	22.444858121744975	27.132217921157824	25.559443785172576	24.863480171924625
44	20.865070007089805	29.040462442355835	27.139611033809906	22.954856516744453
45	18.665966532579613	34.50251934531144	23.62301697130082	23.208497150808125
46	22.17377732450204	31.755188132600846	25.854978724264697	20.216055818632412
47	22.47127112250198	27.935602829350533	26.859304643001124	22.733821405146365
48	22.851352939615328	25.580612099176392	28.90681771364516	22.66121724756312
49	21.562297400278286	25.322105912341698	30.010603113701016	23.104993573679003
50	20.526882495004912	31.20354818856102	26.421088948623556	21.848480367810513
51	19.800904108169455	32.30777591159607	24.521690887009225	23.369629093225257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	882.0
1	1571.0
2	2260.0
3	5218.5
4	8177.0
5	6372.0
6	4567.0
7	4739.0
8	4911.0
9	5164.5
10	5418.0
11	5531.5
12	5645.0
13	5583.5
14	5522.0
15	5345.0
16	5168.0
17	5129.0
18	5090.0
19	5122.0
20	5154.0
21	5747.5
22	6341.0
23	6950.0
24	7559.0
25	8512.0
26	12393.0
27	15321.0
28	17553.5
29	19786.0
30	22643.5
31	25501.0
32	30343.0
33	35185.0
34	39803.5
35	44422.0
36	45609.5
37	46797.0
38	55578.5
39	64360.0
40	85102.0
41	105844.0
42	122776.5
43	139709.0
44	140934.5
45	142160.0
46	140225.5
47	138291.0
48	129912.5
49	121534.0
50	113803.0
51	106072.0
52	99950.0
53	93828.0
54	86160.5
55	78493.0
56	74919.0
57	71345.0
58	67644.0
59	63943.0
60	61212.5
61	58482.0
62	50729.0
63	42976.0
64	37347.0
65	31718.0
66	26860.0
67	22002.0
68	18210.5
69	14419.0
70	11903.0
71	9387.0
72	8116.0
73	6845.0
74	5358.5
75	2960.0
76	2048.0
77	1500.5
78	953.0
79	786.0
80	619.0
81	475.0
82	331.0
83	203.5
84	76.0
85	70.5
86	65.0
87	33.0
88	1.0
89	4.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1582554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.1987282233017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.08956545220337	14.575282351869824
2	6.632879984664582	2.414199603573765
3	2.2352988607970037	1.2203878939650168
4	1.1856864930188364	0.8631194497795804
5	0.6991555275325823	0.6361870715692296
6	0.5098480203246261	0.5567151334245762
7	0.37529496625454695	0.4780923766107778
8	0.3228451374086535	0.4700296731131662
9	0.2620337452652218	0.4291812823874075
>10	4.30086289194472	20.248075906025743
>50	2.3188409197470303	30.704328676868688
>100	1.0617016235255132	25.903915027550457
>500	0.0035213984196534108	0.3706674827054881
>1k	0.0024649788937573876	1.12981807055632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4482	0.28321308467199224	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4147	0.2620447706681731	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2224	0.14053232938654858	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2006	0.12675712803480954	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1943	0.12277622122215102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029446072614267824	0.0
2	0.0	0.0	0.0	0.09819570137891029	0.0
3	0.0	0.0	0.0	0.14615615012189156	0.0
4	0.0	0.0	0.0	0.23639003787548482	0.0
5	0.0	0.0	0.0	0.4058629279000906	0.0
6	0.0	0.0	0.0	0.5477222262241921	0.0
7	0.0	0.0	0.0	0.6395358389034435	0.0
8	0.0	0.0	0.0	0.8421829523668702	0.0
9	0.0	0.0	0.0	0.9121963610720393	0.0
10	0.0	0.0	0.0	1.0708639326051432	0.0
11	0.0	0.0	0.0	1.2223279584772462	0.0
12	0.0	0.0	0.0	1.3771410011917444	0.0
13	0.0	0.0	0.0	1.4367282253875697	0.0
14	0.0	0.0	0.0	1.459097130334889	0.0
15	0.0	0.0	0.0	1.4987798204674216	0.0
16	0.0	0.0	0.0	1.5783347677235657	0.0
17	0.0	0.0	0.0	1.6700220024087646	0.0
18	0.0	0.0	0.0	1.7928614126279419	0.0
19	0.0	0.0	0.0	1.856303165642373	0.0
20	0.0	0.0	0.0	1.919934485647883	0.0
21	0.0	0.0	0.0	1.99961581089808	0.0
22	0.0	0.0	0.0	2.083404420954988	0.0
23	0.0	0.0	0.0	2.1789461844587925	0.0
24	0.0	0.0	0.0	2.2572373517744104	0.0
25	0.0	0.0	0.0	2.324533633607447	0.0
26	0.0	0.0	0.0	2.3909452694821156	0.0
27	0.0	0.0	0.0	2.459252575267574	0.0
28	0.0	0.0	0.0	2.528886849990585	0.0
29	6.31889970263258E-5	0.0	0.0	2.5978892347433327	0.0
30	6.31889970263258E-5	0.0	0.0	2.6896396584255577	0.0
31	6.31889970263258E-5	0.0	0.0	2.7772827973010714	0.0
32	6.31889970263258E-5	0.0	0.0	2.8637253452330853	0.0
33	6.31889970263258E-5	0.0	0.0	2.9467556873256773	0.0
34	6.31889970263258E-5	0.0	0.0	3.034146070213086	0.0
35	6.31889970263258E-5	0.0	0.0	3.143399846071603	0.0
36	6.31889970263258E-5	0.0	0.0	3.2396366885426975	0.0
37	6.31889970263258E-5	0.0	0.0	3.339096169862134	0.0
38	6.31889970263258E-5	0.0	0.0	3.4357753353124125	0.0
39	6.31889970263258E-5	0.0	0.0	3.5303692638608224	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	20	7.0340926E-4	45.000004	41
GCACGAT	20	7.0340926E-4	45.000004	40
TATGCGA	20	7.0340926E-4	45.000004	41
TACGACT	35	1.21232E-7	45.000004	13
CGTAAAC	20	7.0340926E-4	45.000004	40
ACGTATG	20	7.0340926E-4	45.000004	11
ACGTAAG	30	2.1660217E-6	45.000004	1
ACGTAAC	20	7.0340926E-4	45.000004	19
ATACGCG	30	2.1660217E-6	45.000004	1
ATCGCAC	20	7.0340926E-4	45.000004	27
TACACGA	25	3.8914754E-5	45.0	45
CCGCACA	25	3.8914754E-5	45.0	42
CGGACGT	25	3.8914754E-5	45.0	36
CGACGGT	185	0.0	43.783783	28
CGGTCTA	185	0.0	43.783783	31
TTACGAG	100	0.0	42.75	1
GCTACGA	245	0.0	41.32653	10
ATACCGG	60	3.6379788E-12	41.250004	2
CGCACGG	55	6.184564E-11	40.909092	2
CGTTTTT	3670	0.0	40.52452	1
>>END_MODULE