##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546291_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1446920
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203887568075636	31.0	31.0	33.0	30.0	34.0
2	31.421916899344815	31.0	31.0	34.0	30.0	34.0
3	31.49546968733586	31.0	31.0	34.0	30.0	34.0
4	35.28298523760816	37.0	35.0	37.0	33.0	37.0
5	34.995781383905125	37.0	35.0	37.0	32.0	37.0
6	35.13826334558925	37.0	35.0	37.0	32.0	37.0
7	35.68538136178918	37.0	35.0	37.0	35.0	37.0
8	35.76109252757582	37.0	35.0	37.0	35.0	37.0
9	37.480303679539986	39.0	37.0	39.0	35.0	39.0
10	36.82508086141598	39.0	37.0	39.0	32.0	39.0
11	36.47214358775883	39.0	35.0	39.0	32.0	39.0
12	35.33395419235341	37.0	35.0	39.0	30.0	39.0
13	34.73898556934731	37.0	33.0	39.0	27.0	39.0
14	35.622408287949575	37.0	34.0	40.0	27.0	41.0
15	35.99059588643463	37.0	34.0	40.0	30.0	41.0
16	36.194703231692145	37.0	34.0	40.0	31.0	41.0
17	36.179759765571006	37.0	34.0	40.0	31.0	41.0
18	36.154131534569984	37.0	35.0	40.0	31.0	41.0
19	36.05784770408869	37.0	35.0	40.0	30.0	41.0
20	35.95853537168606	36.0	34.0	40.0	30.0	41.0
21	35.78716722417273	36.0	34.0	40.0	30.0	41.0
22	35.64796533325961	36.0	34.0	40.0	30.0	41.0
23	35.65479363060847	36.0	34.0	40.0	30.0	41.0
24	35.5463971746883	35.0	34.0	40.0	30.0	41.0
25	35.39914853620103	35.0	34.0	40.0	29.0	41.0
26	35.31675628231001	35.0	34.0	40.0	29.0	41.0
27	35.235619799297815	35.0	34.0	40.0	29.0	41.0
28	35.25754568324441	36.0	34.0	40.0	29.0	41.0
29	35.32702429989219	36.0	34.0	40.0	29.0	41.0
30	35.24166989190833	36.0	34.0	40.0	29.0	41.0
31	35.10172089680148	36.0	34.0	40.0	29.0	41.0
32	34.91627180493738	35.0	34.0	40.0	28.0	41.0
33	34.747165703701654	35.0	33.0	40.0	27.0	41.0
34	34.62209797362674	35.0	34.0	40.0	27.0	41.0
35	34.4704510270091	35.0	33.0	40.0	26.0	41.0
36	34.26679222071711	35.0	33.0	40.0	24.0	41.0
37	34.159977054709316	35.0	33.0	40.0	24.0	41.0
38	34.16460412462334	35.0	33.0	40.0	24.0	41.0
39	34.148528598678574	35.0	33.0	40.0	24.0	41.0
40	33.93597641887596	35.0	33.0	40.0	23.0	41.0
41	33.92278425897769	35.0	33.0	40.0	23.0	41.0
42	33.88527216432146	35.0	33.0	39.0	23.0	41.0
43	33.816334697149806	35.0	33.0	39.0	23.0	41.0
44	33.59470530506179	35.0	33.0	39.0	23.0	41.0
45	33.57746039863987	35.0	33.0	39.0	23.0	41.0
46	33.533708152489424	35.0	33.0	39.0	23.0	41.0
47	33.52174757415752	35.0	33.0	39.0	23.0	41.0
48	33.459500179692036	35.0	33.0	39.0	23.0	41.0
49	33.39621471815995	35.0	32.0	39.0	24.0	40.0
50	33.290238575733284	35.0	32.0	39.0	24.0	40.0
51	33.19978644292704	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	6.0
14	18.0
15	62.0
16	175.0
17	508.0
18	1080.0
19	2007.0
20	3326.0
21	5351.0
22	7886.0
23	11327.0
24	15022.0
25	20237.0
26	25711.0
27	30279.0
28	34102.0
29	39549.0
30	47337.0
31	57477.0
32	71101.0
33	92401.0
34	159385.0
35	227282.0
36	95793.0
37	116174.0
38	158198.0
39	225006.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.62805130898737	23.975478948386918	29.204240732037707	13.192229010588008
2	30.020733696403397	27.326320736460897	29.681392198601163	12.971553368534542
3	29.36720758576839	26.243192436347552	30.710129101816268	13.679470876067784
4	26.76284798053797	28.065753462527297	29.7972244491748	15.37417410775993
5	23.68354850302712	32.9798468470959	29.236654410748347	14.099950239128631
6	22.072678517126032	41.26731263649683	26.032952754817128	10.627056091560002
7	85.69962402897188	6.007035634312885	6.298482293423271	1.9948580432919583
8	87.20530506178642	4.87974456086031	5.878210267326459	2.0367401100268157
9	83.008943134382	6.60734525751251	7.384098637105023	2.99961297100047
10	56.057833190501206	21.298205844137893	12.093412213529428	10.550548751831476
11	51.73672352306969	19.262778868216625	16.65959417244906	12.340903436264618
12	44.73896276228126	21.515011196196056	21.352528128714788	12.393497912807895
13	22.78142537251541	43.135418682442705	20.742611892848256	13.340544052193625
14	15.235189229535843	44.807245735769776	26.222113178337437	13.735451856356951
15	14.844359052331848	22.454731429519253	49.25089154894535	13.45001796920355
16	15.66969839382965	17.69040444530451	47.115251707074336	19.524645453791504
17	16.04843391479833	18.621278301495593	27.53393414978022	37.796353633925854
18	21.887181046636993	23.399427749979264	33.481118513808646	21.232272689575098
19	31.826707765460426	24.146393719072236	24.354421806319632	19.672476709147706
20	33.36162331020374	24.141210294971387	22.47871340502585	20.01845298979902
21	23.37281950626158	28.41090039532248	26.48266662980676	21.733613468609185
22	22.462126447903135	26.452671882343182	23.65694025930943	27.428261410444254
23	20.299187239100988	31.298620518065963	23.074738064302103	25.32745417853095
24	21.522820888508004	24.7752467309872	36.98048268045227	16.721449700052528
25	17.85274928814309	25.394216680949878	35.04851684958395	21.704517181323084
26	17.345741298758742	35.27271722002598	26.35439416139109	21.027147319824177
27	19.547106958228515	34.49969590578608	27.411121554750785	18.542075581234624
28	15.556630636109805	29.428924888729163	37.272067564205344	17.742376910955684
29	16.08990130760512	25.9138722251403	36.97025405689327	21.02597241036132
30	19.673858955574598	31.147955657534627	30.40264838415393	18.775537002736847
31	30.556907085395185	26.68558040527465	23.154424570812484	19.603087938517678
32	32.584593481325854	26.77141790838471	24.568047991595943	16.0759406186935
33	29.680286401459654	28.21469051502502	22.86166477759655	19.24335830591878
34	19.924667569734332	28.418433638349043	26.923465015342934	24.733433776573687
35	22.214427888203907	25.315359522295633	29.46776601332485	23.0024465761756
36	31.712534210599063	25.04685815387167	24.985209963232244	18.255397672297015
37	20.91981588477594	34.210322616316034	26.86527244077074	18.004589058137284
38	21.597462195560226	34.75942000939928	21.7605672739336	21.882550521106904
39	22.22458739944157	32.726135515439694	24.574475437480995	20.47480164763774
40	24.343640284189867	26.63464462444365	23.493074945401265	25.52864014596522
41	17.743275371133166	25.25640671218865	25.635556907085395	31.36476100959279
42	22.373593564260634	27.090233046747574	22.62260525806541	27.91356813092638
43	23.83158709534736	25.97662621292124	24.433900975865978	25.757885715865424
44	20.74744975534238	30.629129461200343	26.88890885467061	21.734511928786667
45	18.167555911867968	38.04971940397534	21.24243220081276	22.54029248334393
46	22.186782959666047	33.491554474331686	24.87304066568988	19.44862190031239
47	23.44006579492992	28.293824122964644	24.964683603792885	23.301426478312553
48	23.991513006938877	23.925372515412047	29.26644182124789	22.81667265640118
49	21.47506427445885	23.62127830149559	31.404085920437897	23.499571503607662
50	19.69901584054405	34.320971442788824	24.911674453321538	21.06833826334559
51	19.90158405440522	34.68968567716252	22.264741658142814	23.143988610289444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1268.0
1	1748.0
2	2228.0
3	4713.5
4	7199.0
5	5771.0
6	4343.0
7	4712.5
8	5082.0
9	5680.0
10	6278.0
11	6316.0
12	6354.0
13	6253.5
14	6153.0
15	6112.0
16	6071.0
17	5607.0
18	5143.0
19	5709.0
20	6275.0
21	5788.0
22	5301.0
23	5266.5
24	5232.0
25	7102.0
26	9596.5
27	10221.0
28	14168.5
29	18116.0
30	19350.5
31	20585.0
32	22936.5
33	25288.0
34	27414.5
35	29541.0
36	31823.5
37	34106.0
38	40076.5
39	46047.0
40	75079.5
41	104112.0
42	122639.5
43	141167.0
44	148813.0
45	156459.0
46	145413.0
47	134367.0
48	123891.5
49	113416.0
50	106325.0
51	99234.0
52	89842.5
53	80451.0
54	72729.0
55	65007.0
56	65431.0
57	65855.0
58	62370.0
59	58885.0
60	55285.5
61	51686.0
62	46162.5
63	40639.0
64	33468.0
65	26297.0
66	21093.5
67	15890.0
68	14037.0
69	12184.0
70	10280.0
71	8376.0
72	7227.5
73	6079.0
74	4842.0
75	2769.5
76	1934.0
77	1333.0
78	732.0
79	520.0
80	308.0
81	274.5
82	241.0
83	188.0
84	135.0
85	88.5
86	42.0
87	25.0
88	8.0
89	5.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1446920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.120911291076975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14665688066115	14.704513709019217
2	7.440138467091667	2.6964417831099503
3	2.4119878612791332	1.3112225420818096
4	1.1158121806405514	0.8087813417156235
5	0.6545046971411671	0.5930110778244146
6	0.40945972334322617	0.44518699943829115
7	0.3043018248691099	0.38599584619161864
8	0.2323502744531511	0.33683189694583515
9	0.20781619246047414	0.3389236909583068
>10	2.895086622027363	13.457891106716623
>50	1.656668093914732	21.98483402741258
>100	1.5151650536683188	40.713880320483824
>500	0.0069591658499693135	0.7953812589109139
>1k	0.0027063422749880658	0.9216278041903525
>5k	3.8662032499829516E-4	0.505476595000638
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7215	0.49864539850164485	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3565	0.24638542559367485	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2930	0.2024991015398225	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1525	0.10539629005059022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04049982030796451	0.0
2	0.0	0.0	0.0	0.12391839217095624	0.0
3	0.0	0.0	0.0	0.17706576728499157	0.0
4	0.0	0.0	0.0	0.2846736516186106	0.0
5	6.911232134464932E-5	0.0	0.0	0.4469493821358472	0.0
6	6.911232134464932E-5	0.0	0.0	0.6127498410416609	0.0
7	6.911232134464932E-5	0.0	0.0	0.7060514748569375	0.0
8	6.911232134464932E-5	0.0	0.0	0.9224421529870346	0.0
9	6.911232134464932E-5	0.0	0.0	0.9998479528930417	0.0
10	1.3822464268929864E-4	0.0	0.0	1.1447764907527713	0.0
11	1.3822464268929864E-4	0.0	0.0	1.3038039421668095	0.0
12	1.3822464268929864E-4	0.0	0.0	1.4417521355707295	0.0
13	1.3822464268929864E-4	0.0	0.0	1.4980786774666188	0.0
14	1.3822464268929864E-4	0.0	0.0	1.5206092942249745	0.0
15	2.0733696403394796E-4	0.0	0.0	1.560694440604871	0.0
16	2.0733696403394796E-4	0.0	0.0	1.6490890996046774	0.0
17	2.0733696403394796E-4	0.0	0.0	1.7448787769883616	0.0
18	2.0733696403394796E-4	0.0	0.0	1.8918806844884306	0.0
19	2.0733696403394796E-4	0.0	0.0	1.9584358499433279	0.0
20	2.0733696403394796E-4	0.0	0.0	2.0216045116523373	0.0
21	2.0733696403394796E-4	0.0	0.0	2.101498355126752	0.0
22	2.0733696403394796E-4	0.0	0.0	2.17876593039007	0.0
23	2.0733696403394796E-4	0.0	0.0	2.2801537058026704	0.0
24	2.0733696403394796E-4	0.0	0.0	2.3547950128548916	0.0
25	2.0733696403394796E-4	0.0	0.0	2.412089127249606	0.0
26	2.0733696403394796E-4	0.0	0.0	2.471871285212728	0.0
27	2.0733696403394796E-4	0.0	0.0	2.526470019075001	0.0
28	2.0733696403394796E-4	0.0	0.0	2.59067536560418	0.0
29	2.0733696403394796E-4	0.0	0.0	2.6628977414093384	0.0
30	2.0733696403394796E-4	0.0	0.0	2.7570978354020954	0.0
31	2.0733696403394796E-4	0.0	0.0	2.84784231332762	0.0
32	2.0733696403394796E-4	0.0	0.0	2.927114145909933	0.0
33	2.0733696403394796E-4	0.0	0.0	3.008252011168551	0.0
34	2.0733696403394796E-4	0.0	0.0	3.091255909103475	0.0
35	2.0733696403394796E-4	0.0	0.0	3.2005224891493658	0.0
36	2.0733696403394796E-4	0.0	0.0	3.295828380283637	0.0
37	2.0733696403394796E-4	0.0	0.0	3.3856743980316812	0.0
38	2.0733696403394796E-4	0.0	0.0	3.4695767561440856	0.0
39	2.0733696403394796E-4	0.0	0.0	3.5690984988803804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAG	115	0.0	45.000004	1
GGTACGA	40	6.8175723E-9	45.0	8
CTACTCG	20	7.0338935E-4	45.0	45
TTCACGG	35	1.2122291E-7	45.0	1
ATAGCCG	25	3.8913087E-5	45.0	34
CCAATCG	25	3.8913087E-5	45.0	41
TGTATCG	20	7.0338935E-4	45.0	1
CACGATA	25	3.8913087E-5	45.0	10
ACGGGTA	20	7.0338935E-4	45.0	5
CCGTATT	25	3.8913087E-5	45.0	40
TATCTCG	50	2.1827873E-11	45.0	1
TACGAGT	25	3.8913087E-5	45.0	28
TGCGAAC	25	3.8913087E-5	45.0	38
GTCGTAC	20	7.0338935E-4	45.0	30
CGTACTC	20	7.0338935E-4	45.0	32
CTACCGA	20	7.0338935E-4	45.0	33
TCGTACA	35	1.2122291E-7	45.0	34
CGTAATT	45	3.8562575E-10	45.0	33
CGTAAGG	165	0.0	45.0	2
GTATACG	25	3.8913087E-5	45.0	1
>>END_MODULE