FastQCFastQC Report
Thu 26 May 2016
SRR1546290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546290_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2826750
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC88120.31173609268594676No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73650.2605465640753516No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT34120.12070398867957903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG600.045.0000041
TCGTATA351.2127748E-745.00000423
TACGTAA302.166631E-645.00000413
CGACGAA207.0350355E-445.022
GATCGCT253.8922575E-544.9999969
CCGTGTA502.1827873E-1144.99999616
GTTAGCG2500.043.1999971
CGTTTTT72150.042.5987551
TAATACG850.042.352941
TATCGTG700.041.7857171
CGGTCTA4550.041.5384631
AATACGG2250.041.02
TAGGGCG11150.040.7623335
TCGATAG1800.040.01
CGACGGA403.4606092E-739.37512
TACCGTA403.4606092E-739.37519
CTAGCGA403.4606092E-739.37529
TCACGAC4950.039.09090825
CGGCTAC10550.038.8151725
CTCACGA5000.038.69999724
Produced by FastQC (version 0.11.5)