FastQCFastQC Report
Thu 26 May 2016
SRR1546289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546289_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1753022
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67170.3831668969356916No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41730.23804607129859182No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19500.1112364819152298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA302.1661563E-645.00000412
CGACAGT253.8916463E-545.00000424
CTACGTT253.8916463E-545.00000428
ACCGTGC302.1661563E-645.00000425
CCAACGA302.1661563E-645.00000445
TCGAACA502.1827873E-1145.00000424
ACGCCCG207.0343027E-445.032
GACGTAT207.0343027E-445.021
CACGCTA207.0343027E-445.040
CGACACG207.0343027E-445.011
AATGCGC207.0343027E-445.042
ATATCGT207.0343027E-445.012
TGTTACG453.8562575E-1045.01
CGTCGAA207.0343027E-445.034
TCGCGAT207.0343027E-445.01
CGTTTTT44750.042.9888271
GCGATAT2100.042.8571439
GGCGATA6000.041.6258
CCCGAAC650.041.5384641
CGACGGT2250.041.028