FastQCFastQC Report
Thu 26 May 2016
SRR1546287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546287_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1654562
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53590.32389236547194966No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35130.21232205260364978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA351.2123564E-745.0000048
CATTACG351.2123564E-745.00000411
TACTACG351.2123564E-745.00000416
CGTCCCG351.2123564E-745.00000416
TCGACGC351.2123564E-745.00000426
TCGAATA453.8562575E-1045.00000424
ACGCGAA207.034187E-445.02
AATCCGT253.891552E-545.042
CGTATGA207.034187E-445.015
ACGCATA253.891552E-545.036
CGACGAA253.891552E-545.010
ACGTTCA207.034187E-445.036
ACGTTAG750.045.01
CTATCGA253.891552E-545.031
CCGGCAC207.034187E-445.021
TAGCGTC207.034187E-445.01
CCGTGAG207.034187E-445.01
ATTCGAC207.034187E-445.021
CAATTCG207.034187E-445.040
CGCGAAA253.891552E-545.037