##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546287_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1654562
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333300897760253	31.0	31.0	33.0	30.0	34.0
2	31.55864996295092	31.0	31.0	34.0	30.0	34.0
3	31.61356540280751	31.0	31.0	34.0	30.0	34.0
4	35.385072303123124	37.0	35.0	37.0	33.0	37.0
5	35.12300717652164	37.0	35.0	37.0	32.0	37.0
6	35.26564250841008	37.0	35.0	37.0	32.0	37.0
7	35.7904007223664	37.0	35.0	37.0	35.0	37.0
8	35.868602083209936	37.0	35.0	37.0	35.0	37.0
9	37.62771053608145	39.0	37.0	39.0	35.0	39.0
10	36.9695846997574	39.0	37.0	39.0	33.0	39.0
11	36.62415551668659	39.0	35.0	39.0	32.0	39.0
12	35.42933960770282	37.0	35.0	39.0	31.0	39.0
13	34.76474740747098	37.0	34.0	39.0	27.0	39.0
14	35.640454694354155	37.0	34.0	40.0	27.0	41.0
15	36.04448367604236	37.0	35.0	40.0	30.0	41.0
16	36.2747524722555	37.0	35.0	40.0	31.0	41.0
17	36.25354021185063	37.0	35.0	40.0	31.0	41.0
18	36.22098658134298	36.0	35.0	40.0	31.0	41.0
19	36.13161851897964	36.0	35.0	40.0	31.0	41.0
20	36.03353757671214	36.0	34.0	40.0	31.0	41.0
21	35.87240248476636	36.0	34.0	40.0	31.0	41.0
22	35.736554447642334	36.0	34.0	40.0	30.0	41.0
23	35.75803928773899	35.0	34.0	40.0	30.0	41.0
24	35.66641141280895	35.0	34.0	40.0	30.0	41.0
25	35.54489586972262	35.0	34.0	40.0	30.0	41.0
26	35.47989981638645	35.0	34.0	40.0	30.0	41.0
27	35.38401945650873	35.0	34.0	40.0	30.0	41.0
28	35.39180641160621	36.0	34.0	40.0	30.0	41.0
29	35.48894027543241	36.0	34.0	40.0	30.0	41.0
30	35.439340441760415	36.0	34.0	40.0	30.0	41.0
31	35.271234320623826	35.0	34.0	40.0	29.0	41.0
32	35.06982754348281	35.0	34.0	40.0	29.0	41.0
33	34.89050274332421	35.0	34.0	40.0	28.0	41.0
34	34.78336502349262	35.0	34.0	40.0	27.0	41.0
35	34.6122006911799	35.0	34.0	40.0	27.0	41.0
36	34.42184940787955	35.0	33.0	40.0	25.0	41.0
37	34.35944920770572	35.0	33.0	40.0	25.0	41.0
38	34.35856438138915	35.0	33.0	40.0	26.0	41.0
39	34.32620475993042	35.0	33.0	40.0	26.0	41.0
40	34.0953497058436	35.0	33.0	40.0	24.0	41.0
41	34.03812489347634	35.0	33.0	39.0	24.0	41.0
42	34.02690923640214	35.0	33.0	39.0	24.0	41.0
43	33.97809148282143	35.0	33.0	39.0	24.0	41.0
44	33.77339259574437	35.0	33.0	39.0	23.0	41.0
45	33.770807621594116	35.0	33.0	39.0	23.0	41.0
46	33.75837774589287	35.0	33.0	39.0	24.0	41.0
47	33.72616982621383	35.0	33.0	39.0	24.0	41.0
48	33.67524577501478	35.0	33.0	39.0	23.0	41.0
49	33.62644252678352	35.0	33.0	39.0	24.0	41.0
50	33.534526358033126	35.0	33.0	39.0	24.0	40.0
51	33.424904597107876	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	2.0
14	13.0
15	41.0
16	173.0
17	412.0
18	863.0
19	1713.0
20	3083.0
21	4956.0
22	7629.0
23	10914.0
24	15499.0
25	21050.0
26	27502.0
27	32282.0
28	36057.0
29	42482.0
30	50852.0
31	63485.0
32	80625.0
33	105952.0
34	189248.0
35	280514.0
36	107233.0
37	128313.0
38	179087.0
39	264467.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.81007299817112	24.172620911153526	29.48000739772822	13.537298692947136
2	29.60771491186187	25.822423094450375	30.61698503894082	13.952876954746937
3	28.45375392399922	26.31675331598332	31.448141562540417	13.781351197477038
4	25.403641567979925	28.431451949216775	30.52989250327277	15.635013979530536
5	23.731476970944577	31.647408800637265	30.687033789002772	13.934080439415386
6	21.943027822469027	40.42169468415206	27.052658044848123	10.582619448530789
7	86.35965288698762	5.256255129756394	6.315387395576594	2.068704587679398
8	88.8434522248184	3.8183519263708465	5.616229551990195	1.721966296820548
9	85.38912413073672	4.628475693265045	6.999919011798893	2.982481164199347
10	58.29687856967583	20.25678094867403	11.627971632371588	9.81836884927854
11	53.90568621786309	19.115753897406083	16.229128917502035	10.749430967228788
12	49.00849892599975	20.43821869473613	19.254763496321083	11.29851888294304
13	22.326512998606276	44.32514466064131	19.262862316431782	14.085480024320635
14	14.038337638601636	47.3208619562156	24.716692393515626	13.924108011667135
15	13.121418236367086	23.094208618353377	51.533336314988496	12.251036830291037
16	14.110259996301137	16.177211854255084	51.25223473039995	18.460293419043833
17	15.065135062935084	17.69048243583498	27.461225387746126	39.78315711348381
18	21.38620372038038	23.280904553591828	32.959175902746466	22.37371582328133
19	31.909955625718467	23.415743864539376	23.571555493236275	21.102745016505878
20	35.78651026676546	21.920181897082127	22.252596155357125	20.04071168079528
21	23.557291899608476	29.18699933879782	25.370944092756876	21.88476466883683
22	22.404418812954727	26.11065647585282	23.579593874390927	27.90533083680152
23	20.68843597278313	32.235479843003766	22.573345695114476	24.502738489098626
24	20.67906793459538	23.279272701778478	37.868571863731916	18.17308749989423
25	17.655186085501782	23.877557927717426	36.59645271679151	21.870803269989278
26	16.611465753474334	35.71458790906596	26.600937287330424	21.07300905012928
27	19.214208956811532	35.12403886950142	27.07163587704782	18.59011629663923
28	14.798478388842485	30.113347218176166	38.52499936539096	16.563175027590383
29	16.134118878591433	24.723159361812975	38.67863519167006	20.46408656792553
30	19.36639424814543	30.165385159335216	30.312614456273018	20.15560613624633
31	30.187505817249523	27.07979513611457	23.154345379623127	19.57835366701278
32	33.11571279891597	26.113134473050874	25.103562151191678	15.667590576841484
33	30.46751950062917	27.684486891394823	23.943013317119576	17.904980290856432
34	21.317242871527327	27.36029233114262	27.38114377097987	23.941321026350177
35	20.24777554422258	24.665500597741275	31.277220194831017	23.809503663205124
36	31.975592331988768	24.431299643047524	25.233808101479426	18.359299923484283
37	23.154466257535226	31.71346857959992	27.332792606139872	17.799272556724983
38	21.28055642520498	33.58526304846842	21.762254904923477	23.37192562140313
39	22.006005214673127	31.804670964279367	25.825324164340774	20.36399965670673
40	25.863582023520426	25.050859381516076	24.828504462208127	24.257054132755375
41	17.24933849562603	25.199237018618824	26.376346126648624	31.175078359106518
42	23.898046733818376	25.490552786779823	22.34651829305883	28.26488218634297
43	23.175136380504327	27.835040330915373	23.922464072062578	25.067359216517726
44	21.78256239415628	30.08566617630527	27.230046380854873	20.901725048683577
45	17.416512648060333	37.852978613071016	20.95194982116113	23.778558917707528
46	21.294578263008578	34.81356395227257	23.991666676739825	19.90019110797903
47	23.211822826826676	27.604405274628576	25.668908146083375	23.514863752461377
48	24.444898408158775	21.839556329711428	30.371240243641516	23.344305018488278
49	21.603300450511977	22.560230441651626	32.10608003810072	23.730389069735676
50	19.163500672685583	33.602790345722916	24.635945948232827	22.597763033358678
51	18.335003463152184	35.73489539829877	21.829644340919227	24.10045679762983
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1477.0
1	1735.5
2	1994.0
3	4562.0
4	7130.0
5	5682.0
6	4234.0
7	4805.0
8	5376.0
9	6068.5
10	6761.0
11	6909.5
12	7058.0
13	6918.0
14	6778.0
15	6750.5
16	6723.0
17	6403.0
18	6083.0
19	6270.0
20	6457.0
21	6272.0
22	6087.0
23	6308.5
24	6530.0
25	6775.0
26	8882.0
27	10744.0
28	11176.0
29	11608.0
30	15242.0
31	18876.0
32	20741.5
33	22607.0
34	27962.5
35	33318.0
36	35933.5
37	38549.0
38	52102.5
39	65656.0
40	91846.5
41	118037.0
42	142068.5
43	166100.0
44	173793.5
45	181487.0
46	166948.5
47	152410.0
48	139879.5
49	127349.0
50	123073.0
51	118797.0
52	110977.5
53	103158.0
54	92973.0
55	82788.0
56	75097.5
57	67407.0
58	70382.0
59	73357.0
60	69926.0
61	66495.0
62	56608.5
63	46722.0
64	37005.5
65	27289.0
66	22161.0
67	17033.0
68	13498.5
69	9964.0
70	7581.5
71	5199.0
72	4995.5
73	4792.0
74	3618.5
75	1910.0
76	1375.0
77	1032.0
78	689.0
79	578.0
80	467.0
81	284.0
82	101.0
83	59.5
84	18.0
85	12.0
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1654562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.722758815603697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.23251649560133	13.751545401560936
2	7.429358007245806	2.484787242198915
3	2.17479708001228	1.0910582112577358
4	1.0069427551287153	0.6735544334054799
5	0.5879555929428029	0.49161197875338775
6	0.35358810600440826	0.35477811700667
7	0.2670279123638802	0.31258103628467304
8	0.20625968628098448	0.2759384789647189
9	0.17150956763829134	0.2581301820765254
>10	2.3542516920243997	9.885351411061817
>50	1.3313420075544953	16.41515614403148
>100	1.874567612427028	52.493080875155925
>500	0.008785319800478163	0.9038932094296185
>1k	7.321099833731802E-4	0.28048480872318243
>5k	3.660549916865901E-4	0.3280484700889425
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5359	0.32389236547194966	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3513	0.21232205260364978	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034389765992449965	0.0
2	0.0	0.0	0.0	0.15424021583959985	0.0
3	0.0	0.0	0.0	0.23178339645174978	0.0
4	0.0	0.0	0.0	0.36916114355339963	0.0
5	0.0	0.0	0.0	0.6046313163241994	0.0
6	0.0	0.0	0.0	0.8179203922246492	0.0
7	0.0	0.0	0.0	0.9352928448737491	0.0
8	0.0	0.0	0.0	1.185691439788899	0.0
9	0.0	0.0	0.0	1.2811245513918488	0.0
10	0.0	0.0	0.0	1.4743478938836985	0.0
11	0.0	0.0	0.0	1.6917468187955482	0.0
12	1.2087791209999987E-4	0.0	0.0	1.8809811901880982	0.0
13	1.8131686814999983E-4	0.0	0.0	1.956167251514298	0.0
14	1.8131686814999983E-4	0.0	0.0	1.982941709044448	0.0
15	1.8131686814999983E-4	0.0	0.0	2.028996193554548	0.0
16	1.8131686814999983E-4	0.0	0.0	2.135912706806998	0.0
17	1.8131686814999983E-4	0.0	0.0	2.2519555024229976	0.0
18	1.8131686814999983E-4	0.0	0.0	2.4260196958469975	0.0
19	1.8131686814999983E-4	0.0	0.0	2.5029584868986476	0.0
20	1.8131686814999983E-4	0.0	0.0	2.580924740203147	0.0
21	1.8131686814999983E-4	0.0	0.0	2.6833687707078973	0.0
22	1.8131686814999983E-4	0.0	0.0	2.782005146981497	0.0
23	1.8131686814999983E-4	0.0	0.0	2.895751262267597	0.0
24	1.8131686814999983E-4	0.0	0.0	2.976981219198797	0.0
25	1.8131686814999983E-4	0.0	0.0	3.044370655194547	0.0
26	1.8131686814999983E-4	0.0	0.0	3.113452381959697	0.0
27	1.8131686814999983E-4	0.0	0.0	3.181506646471997	0.0
28	1.8131686814999983E-4	0.0	0.0	3.2528246146109967	0.0
29	1.8131686814999983E-4	0.0	0.0	3.3250491670907465	0.0
30	1.8131686814999983E-4	0.0	0.0	3.4236251044082966	0.0
31	1.8131686814999983E-4	0.0	0.0	3.5317503967817463	0.0
32	1.8131686814999983E-4	0.0	0.0	3.6212000517357463	0.0
33	1.8131686814999983E-4	0.0	0.0	3.702973959271396	0.0
34	1.8131686814999983E-4	0.0	0.0	3.7924840531814463	0.0
35	1.8131686814999983E-4	0.0	0.0	3.912032308248346	0.0
36	1.8131686814999983E-4	0.0	0.0	4.015020289357546	0.0
37	1.8131686814999983E-4	0.0	0.0	4.111903935905696	0.0
38	1.8131686814999983E-4	0.0	0.0	4.202743686848846	0.0
39	1.8131686814999983E-4	0.0	0.0	4.302286647463196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	35	1.2123564E-7	45.000004	8
CATTACG	35	1.2123564E-7	45.000004	11
TACTACG	35	1.2123564E-7	45.000004	16
CGTCCCG	35	1.2123564E-7	45.000004	16
TCGACGC	35	1.2123564E-7	45.000004	26
TCGAATA	45	3.8562575E-10	45.000004	24
ACGCGAA	20	7.034187E-4	45.0	2
AATCCGT	25	3.891552E-5	45.0	42
CGTATGA	20	7.034187E-4	45.0	15
ACGCATA	25	3.891552E-5	45.0	36
CGACGAA	25	3.891552E-5	45.0	10
ACGTTCA	20	7.034187E-4	45.0	36
ACGTTAG	75	0.0	45.0	1
CTATCGA	25	3.891552E-5	45.0	31
CCGGCAC	20	7.034187E-4	45.0	21
TAGCGTC	20	7.034187E-4	45.0	1
CCGTGAG	20	7.034187E-4	45.0	1
ATTCGAC	20	7.034187E-4	45.0	21
CAATTCG	20	7.034187E-4	45.0	40
CGCGAAA	25	3.891552E-5	45.0	37
>>END_MODULE