FastQCFastQC Report
Thu 26 May 2016
SRR1546286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546286_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1452744
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52880.36400081500938913No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.22295738271849685No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20470.14090576178597194No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18540.12762055806115877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC302.165898E-645.00000410
CGATCTA302.165898E-645.00000411
GTCGACG351.2122291E-745.012
ACGCATT351.2122291E-745.032
GCGAATC253.891316E-545.020
TTTAACG453.8562575E-1045.01
GACCGTA207.0339016E-445.030
CACGAGT207.0339016E-445.038
TACGGAC253.891316E-545.022
TACGCAT351.2122291E-745.031
CGTAAGA207.0339016E-445.022
AACCTCG351.2122291E-745.013
TATAGCG1000.045.01
ACCGTTA253.891316E-545.027
ATCGGCC351.2122291E-745.024
ATCGGAC253.891316E-545.025
TATGTCG406.8175723E-945.01
TAATGCG700.045.01
TGGTCGA207.0339016E-445.011
AATGTCG351.2122291E-745.018
Produced by FastQC (version 0.11.5)