##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546286_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1452744
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25774878436944	31.0	31.0	33.0	30.0	34.0
2	31.495200806198476	31.0	31.0	34.0	30.0	34.0
3	31.58302288634474	31.0	31.0	34.0	30.0	34.0
4	35.35448089959415	37.0	35.0	37.0	33.0	37.0
5	35.07637408930961	37.0	35.0	37.0	32.0	37.0
6	35.21518106424807	37.0	35.0	37.0	32.0	37.0
7	35.72280525681056	37.0	35.0	37.0	35.0	37.0
8	35.80210071423458	37.0	35.0	37.0	35.0	37.0
9	37.52958814491748	39.0	37.0	39.0	35.0	39.0
10	36.86055767568133	39.0	37.0	39.0	32.0	39.0
11	36.5341298948748	39.0	35.0	39.0	32.0	39.0
12	35.44985076517267	37.0	35.0	39.0	31.0	39.0
13	34.8393591713337	37.0	34.0	39.0	27.0	39.0
14	35.76692865363753	37.0	34.0	40.0	29.0	41.0
15	36.12404663175343	37.0	35.0	40.0	30.0	41.0
16	36.31557177314104	37.0	35.0	40.0	31.0	41.0
17	36.28212816573326	37.0	35.0	40.0	31.0	41.0
18	36.2524987196643	37.0	35.0	40.0	31.0	41.0
19	36.187143777568515	37.0	35.0	40.0	31.0	41.0
20	36.09596666721735	36.0	34.0	40.0	31.0	41.0
21	35.917053520785494	36.0	34.0	40.0	30.0	41.0
22	35.81068378186384	36.0	34.0	40.0	30.0	41.0
23	35.82060776021102	36.0	34.0	40.0	30.0	41.0
24	35.70151520157715	36.0	34.0	40.0	30.0	41.0
25	35.562423937046034	36.0	34.0	40.0	30.0	41.0
26	35.521482793940294	36.0	34.0	40.0	30.0	41.0
27	35.427425616626195	36.0	34.0	40.0	29.0	41.0
28	35.454183944315034	36.0	34.0	40.0	29.0	41.0
29	35.50662195128667	36.0	34.0	40.0	30.0	41.0
30	35.445744742363416	36.0	34.0	40.0	29.0	41.0
31	35.31777931968743	36.0	34.0	40.0	29.0	41.0
32	35.12404800845847	35.0	34.0	40.0	29.0	41.0
33	34.97810144113485	35.0	34.0	40.0	28.0	41.0
34	34.86042138188146	35.0	34.0	40.0	27.0	41.0
35	34.72180714564989	35.0	34.0	40.0	27.0	41.0
36	34.532393869807755	35.0	33.0	40.0	25.0	41.0
37	34.42791985373886	35.0	33.0	40.0	25.0	41.0
38	34.423725721806456	35.0	33.0	40.0	26.0	41.0
39	34.377965422675985	35.0	33.0	40.0	25.0	41.0
40	34.194003210476176	35.0	33.0	40.0	24.0	41.0
41	34.17085391507382	35.0	33.0	40.0	24.0	41.0
42	34.161430369012024	35.0	33.0	40.0	24.0	41.0
43	34.094732451140736	35.0	33.0	40.0	24.0	41.0
44	33.89135043751686	35.0	33.0	39.0	23.0	41.0
45	33.88223251997599	35.0	33.0	39.0	24.0	41.0
46	33.860152924396864	35.0	33.0	39.0	24.0	41.0
47	33.84723323586262	35.0	33.0	39.0	24.0	41.0
48	33.78014708716746	35.0	33.0	39.0	24.0	41.0
49	33.716946688473676	35.0	33.0	39.0	24.0	41.0
50	33.644976678616466	35.0	33.0	39.0	24.0	41.0
51	33.53714832069518	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	13.0
15	55.0
16	128.0
17	347.0
18	880.0
19	1657.0
20	2971.0
21	4464.0
22	6854.0
23	9976.0
24	13658.0
25	18812.0
26	24408.0
27	28418.0
28	32101.0
29	37141.0
30	44632.0
31	55454.0
32	69165.0
33	91251.0
34	160082.0
35	229097.0
36	98270.0
37	118497.0
38	163800.0
39	240492.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.48191009565347	24.353017462126843	30.03323365988777	13.131838782331917
2	29.02803246821188	26.66491825125418	31.200128859592606	13.106920420941337
3	28.87872880562577	25.903049677025	31.639917287560642	13.578304229788593
4	26.242063295391343	28.861382322005802	29.8757386022589	15.020815780343957
5	24.300496164499734	32.363513461421974	28.958508863227106	14.37748151085119
6	21.851888563986495	41.10263060800802	26.036108220030506	11.009372607974976
7	85.80031994625344	5.7990258435071835	6.293400626676138	2.107253583563243
8	88.61506225460232	4.124883668423342	5.3368659584895894	1.9231881184847435
9	84.01370096864967	5.608971711464649	7.2893778945223655	3.0879494253633126
10	55.455882109993226	21.631478085609025	12.20813852956887	10.704501274828877
11	50.94510801627816	19.55994999807261	17.074377866988264	12.420564118660963
12	44.99574598139796	21.47260632293095	20.758302908151745	12.773344787519342
13	22.634889560720953	42.28714763234266	21.287095317550786	13.790867489385604
14	15.364785536887435	45.22758311168382	25.77962806936391	13.628003282064839
15	15.143824376490283	22.898941589158174	48.67932684629914	13.277907188052405
16	16.016311201422962	17.7699580930983	47.29091980417747	18.92281090130126
17	16.611116617931305	18.710591817966552	27.24719565181477	37.43109591228737
18	21.978683099018134	24.009942563865348	32.92610397977896	21.085270357337563
19	30.535042650322424	24.742280814789115	24.110373197204737	20.61230333768372
20	33.53653499859576	22.290024945895492	23.371702103054634	20.801737952454115
21	22.571629963710055	28.840594075762834	26.457448800339222	22.130327160187893
22	23.26266706315772	24.714402537542746	24.381377586140434	27.641552813159098
23	20.766563138446966	30.512740028525325	23.28139025182689	25.439306581200817
24	21.30093120329528	24.17583552229436	37.152588480833515	17.370644793576844
25	17.351164417130615	24.885595810411193	35.67414492849394	22.08909484396425
26	17.39983094061996	35.35137642970819	26.399214176757912	20.849578452913935
27	18.144008854966877	34.96362745260004	27.980084584758224	18.912279107674856
28	16.493752512486715	29.08764379684239	37.376027710319235	17.042575980351664
29	16.153981706343306	24.90796726746075	37.41257922937558	21.525471796820362
30	18.80138551596152	30.791935812503784	30.898286277554753	19.508392393979946
31	29.41646979784463	26.816149300909174	23.930575517778767	19.836805383467425
32	30.128708155050028	27.48977108148442	25.16933472105202	17.21218604241353
33	28.981568672801266	27.800699916847016	23.812936071324337	19.404795339027388
34	19.618046951148997	28.287984669012573	27.621384084188268	24.47258429565016
35	21.4045970935003	26.208058680676018	30.00542421789386	22.38192000792982
36	31.02067535642894	24.969712488917526	24.429424592357634	19.580187562295905
37	20.785974679640734	33.12620805868068	27.44310078031642	18.644716481362167
38	21.33280192518434	33.41242503841007	22.411519166487697	22.84325386991789
39	22.136935344424067	32.00770404145534	25.385339743272045	20.470020870848547
40	23.9277532724279	26.56951259134438	24.39521347188493	25.107520664342786
41	17.850357667971785	25.288419707808117	25.830772661941815	31.030449962278283
42	22.844217563452336	26.874590430247864	22.744337612132625	27.536854394167175
43	23.1897705308024	26.586446063449582	24.542658582654617	25.6811248230934
44	20.823627562736448	31.329676804722652	26.636076280473365	21.210619352067535
45	18.247192898404673	37.999330921346086	21.12533247426938	22.628143705979856
46	21.84906631863563	32.66301564487618	25.31719284333647	20.170725193151718
47	22.762441283529654	28.303679106573494	25.446947294223897	23.486932315672963
48	24.397691540973497	23.697843529210928	29.990005121342783	21.914459808472795
49	21.878803147698424	23.856371115626704	31.373731366297157	22.89109437037771
50	19.56194622039396	33.96668649121938	25.52934309141872	20.942024196967946
51	18.901127796776308	34.75629567219001	22.94113759891626	23.401438932117426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1010.0
1	1378.5
2	1747.0
3	4084.0
4	6421.0
5	5124.0
6	3827.0
7	4167.5
8	4508.0
9	4882.5
10	5257.0
11	5367.0
12	5477.0
13	5301.0
14	5125.0
15	5013.5
16	4902.0
17	4597.0
18	4292.0
19	4565.5
20	4839.0
21	5595.0
22	6351.0
23	5715.0
24	5079.0
25	6591.0
26	8958.5
27	9814.0
28	11890.0
29	13966.0
30	18479.0
31	22992.0
32	24888.0
33	26784.0
34	32421.5
35	38059.0
36	36227.5
37	34396.0
38	45119.5
39	55843.0
40	81716.5
41	107590.0
42	127943.5
43	148297.0
44	146662.0
45	145027.0
46	138228.5
47	131430.0
48	125802.0
49	120174.0
50	112513.0
51	104852.0
52	95977.5
53	87103.0
54	79866.0
55	72629.0
56	67707.5
57	62786.0
58	58576.5
59	54367.0
60	50385.0
61	46403.0
62	40786.5
63	35170.0
64	29052.5
65	22935.0
66	20111.5
67	17288.0
68	13844.5
69	10401.0
70	8721.0
71	7041.0
72	5983.5
73	4926.0
74	3743.0
75	1957.0
76	1354.0
77	961.5
78	569.0
79	567.0
80	565.0
81	383.0
82	201.0
83	193.5
84	186.0
85	136.5
86	87.0
87	46.5
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1452744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.43466015607958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.91946992676111	14.282381183392614
2	6.980048648196066	2.433895521084022
3	2.069061204224426	1.0822013681334488
4	0.9319790403441405	0.6499495136395708
5	0.5481512748275802	0.477841559537032
6	0.38304291150817504	0.4006933792404177
7	0.2847055690838876	0.34746213890645716
8	0.24389510404468304	0.3401782602200575
9	0.21337830116403317	0.3348160348928867
>10	3.0965161852427645	13.74206962437847
>50	1.657896559860289	21.266525300116914
>100	1.663081225113993	42.94767579927511
>500	0.006381127002835465	0.6506201681734051
>1k	0.0019941021883860825	0.6759997087722394
>5k	3.9882043767721655E-4	0.3676904402373588
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5288	0.36400081500938913	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3239	0.22295738271849685	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2047	0.14090576178597194	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1854	0.12762055806115877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033866944210404586	0.0
2	0.0	0.0	0.0	0.10986106292643438	0.0
3	0.0	0.0	0.0	0.16183167853386418	0.0
4	0.0	0.0	0.0	0.26116094783389227	0.0
5	6.883525246017192E-5	0.0	0.0	0.4265720594956854	0.0
6	6.883525246017192E-5	0.0	0.0	0.5847554696491605	0.0
7	6.883525246017192E-5	0.0	0.0	0.6730022633031009	0.0
8	6.883525246017192E-5	0.0	0.0	0.8772364573524309	0.0
9	1.3767050492034385E-4	0.0	0.0	0.9491004609208504	0.0
10	1.3767050492034385E-4	0.0	0.0	1.0867709658411944	0.0
11	1.3767050492034385E-4	0.0	0.0	1.2329770420665995	0.0
12	1.3767050492034385E-4	0.0	0.0	1.3617677994195811	0.0
13	1.3767050492034385E-4	0.0	0.0	1.4167671661352585	0.0
14	1.3767050492034385E-4	0.0	0.0	1.4388632821749736	0.0
15	1.3767050492034385E-4	0.0	0.0	1.4773421883002098	0.0
16	1.3767050492034385E-4	0.0	0.0	1.554713012065443	0.0
17	1.3767050492034385E-4	0.0	0.0	1.6481224496538964	0.0
18	1.3767050492034385E-4	0.0	0.0	1.7767067012494975	0.0
19	1.3767050492034385E-4	0.0	0.0	1.8369375471521479	0.0
20	1.3767050492034385E-4	0.0	0.0	1.8946903239662323	0.0
21	1.3767050492034385E-4	0.0	0.0	1.9745392168200315	0.0
22	1.3767050492034385E-4	0.0	0.0	2.052736063614787	0.0
23	1.3767050492034385E-4	0.0	0.0	2.1522030034197352	0.0
24	1.3767050492034385E-4	0.0	0.0	2.225237206279978	0.0
25	1.3767050492034385E-4	0.0	0.0	2.288152627028575	0.0
26	1.3767050492034385E-4	0.0	0.0	2.3507927067673315	0.0
27	1.3767050492034385E-4	0.0	0.0	2.409027330348637	0.0
28	2.0650575738051578E-4	0.0	0.0	2.469189340998827	0.0
29	2.0650575738051578E-4	0.0	0.0	2.5453899654722374	0.0
30	2.0650575738051578E-4	0.0	0.0	2.6420346599263187	0.0
31	2.0650575738051578E-4	0.0	0.0	2.732690687416365	0.0
32	2.0650575738051578E-4	0.0	0.0	2.8137097795619876	0.0
33	2.0650575738051578E-4	0.0	0.0	2.895554894737132	0.0
34	2.0650575738051578E-4	0.0	0.0	2.984903052430435	0.0
35	2.0650575738051578E-4	0.0	0.0	3.095727808891312	0.0
36	2.0650575738051578E-4	0.0	0.0	3.1848006255747743	0.0
37	2.0650575738051578E-4	0.0	0.0	3.284061059622342	0.0
38	2.0650575738051578E-4	0.0	0.0	3.380086236804282	0.0
39	2.0650575738051578E-4	0.0	0.0	3.476593260753443	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAC	30	2.165898E-6	45.000004	10
CGATCTA	30	2.165898E-6	45.000004	11
GTCGACG	35	1.2122291E-7	45.0	12
ACGCATT	35	1.2122291E-7	45.0	32
GCGAATC	25	3.891316E-5	45.0	20
TTTAACG	45	3.8562575E-10	45.0	1
GACCGTA	20	7.0339016E-4	45.0	30
CACGAGT	20	7.0339016E-4	45.0	38
TACGGAC	25	3.891316E-5	45.0	22
TACGCAT	35	1.2122291E-7	45.0	31
CGTAAGA	20	7.0339016E-4	45.0	22
AACCTCG	35	1.2122291E-7	45.0	13
TATAGCG	100	0.0	45.0	1
ACCGTTA	25	3.891316E-5	45.0	27
ATCGGCC	35	1.2122291E-7	45.0	24
ATCGGAC	25	3.891316E-5	45.0	25
TATGTCG	40	6.8175723E-9	45.0	1
TAATGCG	70	0.0	45.0	1
TGGTCGA	20	7.0339016E-4	45.0	11
AATGTCG	35	1.2122291E-7	45.0	18
>>END_MODULE