FastQCFastQC Report
Thu 26 May 2016
SRR1546285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546285_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1518213
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC82560.5437972142248815No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.16605048171765097No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG24960.16440380895170836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG551.8189894E-1245.0000041
TGTACCG650.045.0000041
AATACGG1150.045.0000042
CTAGAAT253.8913997E-545.019
TCGCATA207.034003E-445.016
ACCGGTA351.2122655E-745.041
CCCTCGT207.034003E-445.023
GGTGTCG207.034003E-445.010
CGACGTA351.2122655E-745.036
CTATCGT207.034003E-445.013
ACACCTA406.8175723E-945.036
ACACCGT207.034003E-445.030
GACGTAT253.8913997E-545.045
GACGTAG453.8562575E-1045.01
ACTCTCG253.8913997E-545.045
ATTACAC207.034003E-445.038
CCAATCG253.8913997E-545.020
GCACGTC453.8562575E-1045.032
CCGCTTG351.2122655E-745.038
TACGGGT900.045.04