FastQCFastQC Report
Thu 26 May 2016
SRR1546284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546284_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1762662
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63710.36144195540608465No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44940.25495528921596994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT302.1661635E-645.00000417
CGTTAGT302.1661635E-645.00000432
ACGTTAG700.045.01
CTATCGA253.8916558E-545.037
TACGCCC253.8916558E-545.034
TACCGAA207.034313E-445.032
CGTTTTT54000.042.6666681
TCTAGCG556.184564E-1140.9090921
TATTACG556.184564E-1140.9090921
TCACGAC2050.040.60975625
ACTAACG501.0822987E-940.51
GCGCGAC5450.040.045879
CGACGGT2100.039.64285728
CACAACG3150.039.28571312
TTCACGG356.2501877E-638.5714262
TATCTCG356.2501877E-638.5714261
TGTAACG356.2501877E-638.5714261
TACGGGA5450.038.3944974
GCGATAT1600.037.968759
TTGTACG1900.037.8947371