##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546284_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1762662
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307265374757044	31.0	31.0	33.0	30.0	34.0
2	31.548451716778374	31.0	31.0	34.0	30.0	34.0
3	31.630098112967772	31.0	31.0	34.0	30.0	34.0
4	35.41127567281759	37.0	35.0	37.0	33.0	37.0
5	35.15753672570238	37.0	35.0	37.0	32.0	37.0
6	35.296285391073276	37.0	35.0	37.0	32.0	37.0
7	35.773402955302835	37.0	35.0	37.0	35.0	37.0
8	35.833980649721845	37.0	35.0	37.0	35.0	37.0
9	37.56126926205932	39.0	37.0	39.0	35.0	39.0
10	36.9270591866166	39.0	37.0	39.0	32.0	39.0
11	36.64947562266617	39.0	35.0	39.0	32.0	39.0
12	35.92041185434304	37.0	35.0	39.0	31.0	39.0
13	35.54231100460554	37.0	35.0	39.0	30.0	39.0
14	36.47863572256053	38.0	35.0	41.0	31.0	41.0
15	36.70305027282599	38.0	35.0	41.0	31.0	41.0
16	36.82309313980786	38.0	35.0	41.0	32.0	41.0
17	36.77682959069861	38.0	35.0	40.0	31.0	41.0
18	36.73366930245277	38.0	35.0	40.0	31.0	41.0
19	36.68998310509899	38.0	35.0	40.0	31.0	41.0
20	36.625095452219426	38.0	35.0	40.0	31.0	41.0
21	36.47643677574033	38.0	35.0	40.0	31.0	41.0
22	36.40605686172392	38.0	35.0	40.0	31.0	41.0
23	36.36043325379455	38.0	35.0	40.0	31.0	41.0
24	36.24859161881291	38.0	35.0	40.0	31.0	41.0
25	36.112136076003225	38.0	35.0	40.0	30.0	41.0
26	36.094709592650204	38.0	35.0	40.0	30.0	41.0
27	36.04608370748334	38.0	34.0	40.0	30.0	41.0
28	36.02906229328141	38.0	35.0	40.0	30.0	41.0
29	36.034956219626906	38.0	35.0	40.0	30.0	41.0
30	35.92209283458769	37.0	35.0	40.0	30.0	41.0
31	35.80321751986484	37.0	34.0	40.0	30.0	41.0
32	35.641007181183916	37.0	34.0	40.0	29.0	41.0
33	35.49155198217242	37.0	34.0	40.0	29.0	41.0
34	35.30211691180725	37.0	34.0	40.0	27.0	41.0
35	35.151915682076314	37.0	34.0	40.0	27.0	41.0
36	35.00041414633095	37.0	34.0	40.0	26.0	41.0
37	34.92988445884691	37.0	34.0	40.0	26.0	41.0
38	34.88325612057218	37.0	34.0	40.0	26.0	41.0
39	34.81374194258457	37.0	34.0	40.0	26.0	41.0
40	34.66152898286796	37.0	33.0	40.0	25.0	41.0
41	34.59825706800283	36.0	33.0	40.0	24.0	41.0
42	34.54082858767024	36.0	33.0	40.0	25.0	41.0
43	34.50069213496405	36.0	33.0	40.0	25.0	41.0
44	34.25118825957557	36.0	33.0	40.0	24.0	41.0
45	34.22909270183393	35.0	33.0	40.0	24.0	41.0
46	34.22371674206399	35.0	33.0	40.0	24.0	41.0
47	34.17681608839358	35.0	33.0	40.0	24.0	41.0
48	34.059082796361416	35.0	33.0	40.0	24.0	41.0
49	33.9842499583017	35.0	33.0	39.0	24.0	41.0
50	33.93247656101964	35.0	33.0	39.0	24.0	41.0
51	33.862251526384526	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	3.0
13	5.0
14	13.0
15	58.0
16	166.0
17	411.0
18	871.0
19	1705.0
20	3052.0
21	4942.0
22	7595.0
23	10782.0
24	14983.0
25	21018.0
26	27346.0
27	32127.0
28	36061.0
29	41293.0
30	49807.0
31	61589.0
32	76542.0
33	100532.0
34	163655.0
35	222660.0
36	135456.0
37	169297.0
38	237487.0
39	343080.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.604989498837554	23.830774135937578	28.282960658367855	13.281275706857015
2	31.43472770162402	25.497911681309294	29.60624328430522	13.461117332761471
3	29.770199845461015	25.205115898567055	31.162695967803245	13.861988288168689
4	26.290349482770946	27.646990744680487	30.612505403758632	15.450154368789931
5	23.58092476039082	32.22591739085542	29.70836155768945	14.484796291064312
6	21.522163636590566	40.486775116273	27.35464882093107	10.636412426205364
7	85.63246952620524	5.2045712677756715	7.028857489410903	2.1341017166081757
8	86.87263922408268	4.102998759830302	6.900301929694972	2.1240600863920593
9	82.55541901964189	5.951112578588521	8.37035120743512	3.1231171943344784
10	47.81722190641201	25.187982721588142	14.579993214808058	12.414802157191792
11	41.275638778166204	23.130412977644042	20.775111734410796	14.81883650977896
12	36.64395102407608	22.98931956325149	25.477090900013728	14.889638512658696
13	24.02162184241789	34.86079577366506	25.48667867123703	15.630903712680025
14	18.813306237951462	36.880014432715974	28.56679272600192	15.739886603330644
15	17.15053708538563	24.301993235231713	42.226757029992136	16.320712649390522
16	20.152076802018765	20.998977682618676	39.2011627867396	19.64778272862296
17	20.539388720015523	20.988425461035636	28.299356314483436	30.172829504465405
18	24.572436462577624	22.948869380516516	32.44700345273229	20.031690704173574
19	28.63073011161527	24.872777651075477	25.751448661172706	20.74504357613655
20	30.404694717421716	24.4320238366743	25.589534465484594	19.573746980419386
21	24.367122000701215	26.792714655447273	28.043266377785418	20.796896966066097
22	24.98771744100684	23.979356223711637	26.93131184537932	24.101614489902204
23	21.550302894145332	28.763313669892472	26.333409354714632	23.352974081247567
24	21.582867276880084	25.51912958922357	34.61701676214725	18.280986371749094
25	21.245309650971087	25.708842648221836	31.996434937611408	21.049412763195665
26	20.11225067539891	30.72455184261078	26.990994302934997	22.172203179055316
27	19.65510120488216	30.866893369233576	28.403743882831762	21.074261543052497
28	18.004642977496538	28.266281340381767	34.065975212491104	19.663100469630592
29	19.433050692645555	25.59293840793073	33.83359940816788	21.14041149125584
30	21.068645038016363	27.710304074178715	31.170865429673984	20.05018545813094
31	27.721196690006366	26.398651584932338	26.112380025211866	19.767771699849433
32	28.113557789298234	26.357123487089414	26.562948540332748	18.966370183279608
33	27.19750014466755	27.516052425252262	25.182593146048422	20.103854284031765
34	21.15533210564476	27.165730015170237	29.169857862709925	22.50908001647508
35	21.78613937328881	27.798295986411464	29.281166780698737	21.13439785960099
36	27.936666246847096	26.81234405688669	25.92408527556616	19.326904420700057
37	22.082906422218212	31.208422261329737	27.049882507253237	19.658788809198814
38	22.141851358910557	31.435068095868633	25.10991897482331	21.3131615703975
39	21.646747930119332	30.30320050015261	26.22505052017914	21.82500104954892
40	24.594959215096257	26.071305786361766	26.184600337444163	23.14913466109782
41	20.33503870849885	26.007368400748415	26.072043307225094	27.58554958352764
42	22.66855472007679	27.061228982073704	25.172324586335893	25.097891711513608
43	22.738278807848584	26.704439081344013	26.817563435304102	23.7397186755033
44	22.20652626538724	28.3685130785142	27.928156390731747	21.496804265366816
45	19.958335744459234	33.09806417793088	24.262961361849296	22.680638715760594
46	22.63304025388872	30.53795906418814	26.465595786373108	20.363404895550026
47	22.087274815024095	28.461724369164365	27.28639977488594	22.1646010409256
48	22.47424633877624	27.112117921643513	28.725927035359017	21.68770870422123
49	23.040095038073098	25.892542075565256	29.719991694380433	21.347371191981217
50	20.60667331570091	31.266119085791832	26.789140515878824	21.338067082628434
51	20.17806022935764	31.80059478221009	25.37213600792438	22.64920898050789
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1126.0
1	1714.5
2	2303.0
3	7027.5
4	11752.0
5	8854.5
6	5957.0
7	6201.5
8	6446.0
9	6761.0
10	7076.0
11	7253.0
12	7430.0
13	7360.0
14	7290.0
15	7176.0
16	7062.0
17	7009.0
18	6956.0
19	7169.5
20	7383.0
21	7864.0
22	8345.0
23	8907.5
24	9470.0
25	10734.5
26	14202.5
27	16406.0
28	18687.5
29	20969.0
30	24776.5
31	28584.0
32	33858.0
33	39132.0
34	44381.5
35	49631.0
36	54472.5
37	59314.0
38	67011.0
39	74708.0
40	91961.5
41	109215.0
42	126268.5
43	143322.0
44	145473.5
45	147625.0
46	144646.5
47	141668.0
48	133985.5
49	126303.0
50	124294.5
51	122286.0
52	117131.0
53	111976.0
54	103591.5
55	95207.0
56	91259.5
57	87312.0
58	79901.5
59	72491.0
60	66050.5
61	59610.0
62	53832.0
63	48054.0
64	42470.5
65	36887.0
66	31013.0
67	25139.0
68	20828.0
69	16517.0
70	14255.5
71	11994.0
72	10107.0
73	8220.0
74	6447.5
75	3574.5
76	2474.0
77	1943.5
78	1413.0
79	999.5
80	586.0
81	400.0
82	214.0
83	142.0
84	70.0
85	56.0
86	42.0
87	24.5
88	7.0
89	7.5
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1762662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.691546728050366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58953203742571	15.063430038692546
2	6.058089559390191	2.264701281641116
3	2.0321311057874434	1.1395102056405197
4	1.1420379346624576	0.8538582168379781
5	0.7405358204643467	0.6920879946002225
6	0.5617000367470432	0.6299425490402978
7	0.44339350890255813	0.5801397343396466
8	0.34508168985119836	0.5160088424678606
9	0.3018444272261505	0.5077745294489271
>10	4.511427197794492	21.16389760034285
>50	2.2488618360203994	30.56888965512731
>100	1.0213715134949155	24.9778206461199
>500	0.003378973428089255	0.41810754697268687
>1k	3.071794025535686E-4	0.2580301175632045
>5k	3.071794025535686E-4	0.3658010411649256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6371	0.36144195540608465	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4494	0.25495528921596994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03579812805858412	0.0
2	0.0	0.0	0.0	0.1003595697870607	0.0
3	0.0	0.0	0.0	0.14120687914075417	0.0
4	0.0	0.0	0.0	0.1993008302215626	0.0
5	0.0	0.0	0.0	0.31140400144781016	0.0
6	0.0	0.0	0.0	0.4278188331058365	0.0
7	0.0	0.0	0.0	0.4994718215971071	0.0
8	0.0	0.0	0.0	0.6705199295156984	0.0
9	0.0	0.0	0.0	0.7345707798772538	0.0
10	0.0	0.0	0.0	0.8419651640530061	0.0
11	0.0	0.0	0.0	0.977952664776344	0.0
12	0.0	0.0	0.0	1.0792766849231445	0.0
13	0.0	0.0	0.0	1.1233577396006722	0.0
14	0.0	0.0	0.0	1.1436679295293142	0.0
15	0.0	0.0	0.0	1.1723745108251042	0.0
16	0.0	0.0	0.0	1.2339291367261562	0.0
17	0.0	0.0	0.0	1.306716772699474	0.0
18	0.0	0.0	0.0	1.3959000647883713	0.0
19	0.0	0.0	0.0	1.441285964070253	0.0
20	0.0	0.0	0.0	1.4963730993236366	0.0
21	0.0	0.0	0.0	1.5659269899731203	0.0
22	0.0	0.0	0.0	1.6419483712702718	0.0
23	0.0	0.0	0.0	1.7209765683948481	0.0
24	0.0	0.0	0.0	1.7848572216340965	0.0
25	0.0	0.0	0.0	1.8391501036500475	0.0
26	0.0	0.0	0.0	1.8978681108459818	0.0
27	0.0	0.0	0.0	1.972187520920063	0.0
28	0.0	0.0	0.0	2.0415144820731372	0.0
29	0.0	0.0	0.0	2.1213369324351463	0.0
30	0.0	0.0	0.0	2.2148318849558226	0.0
31	0.0	0.0	0.0	2.293179293591171	0.0
32	0.0	0.0	0.0	2.366874647550126	0.0
33	0.0	0.0	0.0	2.4485125338834104	0.0
34	0.0	0.0	0.0	2.5400218533105043	0.0
35	0.0	0.0	0.0	2.646224857630107	0.0
36	0.0	0.0	0.0	2.730642630294407	0.0
37	0.0	0.0	0.0	2.829583890728909	0.0
38	0.0	0.0	0.0	2.936297486415433	0.0
39	0.0	0.0	0.0	3.1218123497301242	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAT	30	2.1661635E-6	45.000004	17
CGTTAGT	30	2.1661635E-6	45.000004	32
ACGTTAG	70	0.0	45.0	1
CTATCGA	25	3.8916558E-5	45.0	37
TACGCCC	25	3.8916558E-5	45.0	34
TACCGAA	20	7.034313E-4	45.0	32
CGTTTTT	5400	0.0	42.666668	1
TCTAGCG	55	6.184564E-11	40.909092	1
TATTACG	55	6.184564E-11	40.909092	1
TCACGAC	205	0.0	40.609756	25
ACTAACG	50	1.0822987E-9	40.5	1
GCGCGAC	545	0.0	40.04587	9
CGACGGT	210	0.0	39.642857	28
CACAACG	315	0.0	39.285713	12
TTCACGG	35	6.2501877E-6	38.571426	2
TATCTCG	35	6.2501877E-6	38.571426	1
TGTAACG	35	6.2501877E-6	38.571426	1
TACGGGA	545	0.0	38.394497	4
GCGATAT	160	0.0	37.96875	9
TTGTACG	190	0.0	37.894737	1
>>END_MODULE