FastQCFastQC Report
Thu 26 May 2016
SRR1546283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546283_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2788333
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC86120.308858375237104No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.22436344582946155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG253.892242E-545.01
TCCGCAT253.892242E-545.011
CGTATCA253.892242E-545.032
CGAGTGT207.0350204E-445.019
CGAAACG207.0350204E-445.045
TACGCGC207.0350204E-445.034
TCGTGAC351.2127566E-745.016
GAACGTT700.045.038
GAACGTA700.045.026
ACGTACT406.8212103E-945.017
TCGAATA351.2127566E-745.036
TCTAGCG600.044.9999961
CTACGCG302.16662E-644.9999961
CGGACTA302.16662E-644.99999624
CGGTCTA2650.044.15094431
ACGTTAG2200.042.9545441
CTATACG850.042.352941
TAAGCGT800.042.187536
CGTTTTT63250.042.0830041
TCGATTG1250.041.3999981
Produced by FastQC (version 0.11.5)