FastQCFastQC Report
Thu 26 May 2016
SRR1546282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546282_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2208954
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196330.8887917086548656No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29560.13381899306187453No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27300.12358790631221836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC302.1664146E-644.99999623
CGTTTTT153550.043.4760671
CGCATCG1200.039.37499621
CGTTCGA750.039.014
CGTACCG356.250906E-638.57142641
CGTAAGG3450.037.8260882
ATCGACG301.1402627E-437.4999961
GTTTTTT187250.036.9492652
TAAGGGA20000.036.78754
CGGTCTA2150.036.62790731
AGGGCGA20150.036.625316
GGGCGAT37700.036.4655157
TCACGAC2100.036.4285725
ATTCGGC2100.036.4285717
ATACTAT18500.036.36486445
TATTGCG1800.036.2500041
CTCGATC3050.036.1475416
AACGGGA6500.036.04
TACGATA250.00210752836.023
GTAGGGA21500.035.9999964
Produced by FastQC (version 0.11.5)